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Aedes aegypti control in breeding sites through an insecticidal coating with dual effect: Laboratory trials and safety assessment. 通过具有双重效果的杀虫涂层在埃及伊蚊繁殖地进行控制:实验室试验和安全评估。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-11-14 DOI: 10.1111/mve.12776
Rocio Cárdenas, Olga L Cabrera, Maria A Carrillo, Alejandra Pineda, Martha L Ahumada, Yohana Yañez, Hanafy Ismail, Mark Paine, Tatiana Rivera, Axel Kroeger

Ground water tanks are known to be preferred Aedes aegypti oviposition places providing opportunities for adult and larvae control. Therefore, a dual-effect insecticidal coating (IC) (alphacypermethrin/ pyriproxyfen) with a slow-release mechanism and safe for users could be applied within Aedes spp. breeding sites, representing a promising option. Bioassays were designed to determine the mortality and sterilizing effects on gravid mosquitoes exposed to IC. The effect of inhibition of emergence was evaluated in eggs, larvae and pupae exposed in different containers. For the water safety assessment concentrations of active ingredients were determined by reverse phase high performance liquid chromatography (RP-HPLC) and the health risk was calculated. The IC applied to the interior walls of water-holding containers showed efficacy against Ae. aegypti in terms of high gravid-female mortality (81% at 24 h, p < 0.01), sterilizing effect (inhibition of oviposition by 63%, p < 0.01) and emergence inhibition (100% in eggs, L3 and L4; 97% in pupae). The offspring rate was reduced [only 0.15 (38/250) new adults emerged per exposed gravid females as against 11.90 per unexposed female (2976/250) at baseline]. Emergence inhibition was recorded up to 12 months and adult mortality >80% up to 6 months. The use of water stored in treated containers, either for washing or drinking, is not expected to pose a health risk to users. IC applied to domestic water containers has dual and complementary action that reduces Ae. aegypti densities (immature and adult stages). This represents baseline information for a cluster randomized efficacy trial in Colombia.

众所周知,地下水池是埃及伊蚊的首选产卵地,这为成虫和幼虫控制提供了机会。因此,一种具有缓释机制且对使用者安全的双效杀虫涂层(IC)(alphacypermethrin/ pyriproxyfen)可用于伊蚊繁殖地,是一种很有前景的选择。设计了生物测定,以确定接触集成电路的雌蚊的死亡率和绝育效果。对暴露在不同容器中的卵、幼虫和蛹的出苗抑制效果进行了评估。在水安全评估中,采用反相高效液相色谱法(RP-HPLC)测定了活性成分的浓度,并计算了健康风险。应用于盛水容器内壁的 IC 对埃及蚁有很高的杀灭率(24 小时内 81%,6 个月内 80%)。使用经过处理的容器储存的水,无论是用于洗涤还是饮用,预计都不会对使用者的健康造成危害。将集成电路应用于家用水容器具有双重和互补作用,可降低埃及蚁的密度(幼虫和成虫阶段)。这是在哥伦比亚进行分组随机疗效试验的基线信息。
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引用次数: 0
A multiplex qPCR followed by high-resolution melting analysis for the detection of blood-feeding sources in Culex sp. mosquitoes. 利用多重 qPCR 和高分辨率熔解分析检测库蚊的嗜血源。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-11-11 DOI: 10.1111/mve.12775
Thamyris Sampaio Vilela, Nathália Alves Senne, Patrícia Gonzaga Paulino, Thamires Rezende Araújo, Pamella Pryscila de Alvarenga Bissoli Maciel de Lima, Natalia Pedra Gonçalves, Daniel da Silva Guedes-Junior, Isabele da Costa Angelo, Carlos Luiz Massard, Maristela Peckle Peixoto, Huarrisson Azevedo Santos

Culex species, such as Culex quinquefasciatus and Culex nigripalpus display a range of feeding habits and act as vectors for pathogens that can cause diseases in both humans and animals. Understanding their feeding habits is pivotal for enhancing disease prevention strategies. The present study introduces the application of two multiplex real-time PCR (qPCR) followed by high-resolution melting (HRM) as a cost-effective and time-efficient alternative. This investigation involved the development of two multiplex qPCR-HRM: assay 1 detects human, dog and chicken, while assay 2 detects cat, cattle and horse in Culex sp. engorged female mosquitoes. The qPCR-HRM reactions showed a detection limit of one copy of genomic DNA when performed as single and multiplex qPCR-HRM. The reaction efficiencies were 97.96% for human, 100.60% for dog, 99.03% for chicken, 99.92% for feline, 99.18% for cattle and 97.68% for horse. The qPCR-HRM method, employing multiplex 1 and 2, was applied to field-collected mosquitoes and demonstrated the ability to detect DNA from multiple blood sources within a single sample. By analysing both multiplexes, it was possible to identify up to five distinct blood sources in Cx. quinquefasciatus and Cx. nigripalpus, and up to two sources in Culex coronatus. Sequencing corroborated the qPCR-HRM results, confirming the presence of DNA from one to four different blood sources with 100% accuracy. The development of these molecular methods may contribute for identification of blood-feeding patterns in mosquitoes. It contributes to studies on the dissemination and transmission of pathogens among various animals and humans, thereby bridging the gap between epidemiology and vector monitoring.

库蚊物种,如五带喙库蚊和黑带喙库蚊,有各种取食习性,是可导致人类和动物疾病的病原体的传播媒介。了解它们的取食习性对于加强疾病预防策略至关重要。本研究介绍了两种多重实时 PCR(qPCR)和高分辨率熔融(HRM)的应用,这是一种具有成本效益和时间效率的替代方法。这项调查涉及开发两种多重 qPCR-HRM:检测 1 检测人、狗和鸡,检测 2 检测库蚊吞食雌蚊中的猫、牛和马。qPCR-HRM 反应在进行单倍和多倍 qPCR-HRM 时,基因组 DNA 的检测限均为一个拷贝。反应效率分别为:人 97.96%、狗 100.60%、鸡 99.03%、猫 99.92%、牛 99.18%、马 97.68%。qPCR-HRM 方法采用了多重酶 1 和 2,适用于野外采集的蚊子,证明了在单一样本中检测多种血液来源 DNA 的能力。通过分析这两种多重因子,可以确定五步蛇和黑线蝇最多有五个不同的血液来源,冠状库蚊最多有两个来源。测序证实了 qPCR-HRM 的结果,以 100% 的准确率确认了一到四个不同血源中 DNA 的存在。这些分子方法的开发可能有助于确定蚊子的吸血模式。它有助于研究病原体在各种动物和人类之间的传播和传染,从而缩小流行病学和病媒监测之间的差距。
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引用次数: 0
Environmental determinants of West Nile virus vector abundance at the wildlife-livestock interface. 野生动物与家畜交界处西尼罗河病毒病媒数量的环境决定因素。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-11-05 DOI: 10.1111/mve.12774
Laia Casades-Martí, Alfonso Peralbo-Moreno, Sarah Delacour-Estrella, Francisco Ruiz-Fons

The diversity and abundance of vectors are essential parameters in the transmission dynamics of West Nile virus (WNV) between its avian reservoirs and clinically susceptible mammalian species. Knowing the determinants of vector abundance could be thus useful in preventing West Nile fever (WNF) cases and associated socio-economic impact. We designed a survey at the wildlife-livestock interface to test the hypothesis that variations in environmental favourability between anthropized and wild scenarios modulate WNV vector abundance and transmission risk. In a continental Mediterranean region where WNF has recently emerged, we selected nine sampling sites and allocated three areas to every site with a decreasing gradient of wildlife-livestock interaction: A1-a horse farm where interaction is maximal; A2-a zone of intermediate interaction 500-1000 m from the farm; and A3-an entirely wild zone of low interaction 1-5 km from the farm. At a fortnightly frequency, we estimated mosquito abundance at each of the 27 study sites in May-December 2018 and April-July 2019. We estimated bird and mammal abundance, collected meteorological information and characterised mosquito habitat at the site scale. Thereafter, we studied the determinants of Culex spp., Culex pipiens sensu lato (s.l.) Linnaeus, 1758 (Diptera: Culicidae) and Culex theileri  Theobald, 1903 abundance by constructing negative binomial generalised linear mixed models. We identified 20 mosquito species, with a notable predominance of Culex spp. and, particularly, of Cx. pipiens s.l. We found differences in the spatiotemporal distribution of Culex spp. abundance and confirmed our hypothesis by finding important effects of local environmental variations in abundance. The accumulated rainfall in fortnights 4-14 and the mean temperature of the two fortnights before sampling were positively and statistically significantly associated with the abundance of Cx. pipiens s.l. (Z = 13.09, p < 0.001, and Z = 9.91, p < 0. 001, respectively) and Culex spp. (Z = 13.35, p < 0.001, and Z = 6.99, p < 0.001, respectively), while the mean temperature of the two previous fortnights was a positive statistically significant predictor (Z = 14.69, p < 0.001) of the abundance of Cx. theileri. The farm environment was the most conducive predictor to hosting Culex spp. compared with wild settings. Our results indicate that continental Mediterranean environments are favourable for WNV circulation and maintenance, especially the environment of anthropized rural settings such as farms. These results will have an impact on the spatiotemporal risk prediction of WNF emergence in continental Mediterranean environments.

病媒的多样性和丰度是西尼罗河病毒(WNV)在禽类水库和临床易感哺乳动物物种之间传播动态的重要参数。因此,了解病媒数量的决定因素有助于预防西尼罗河热(WNF)病例和相关的社会经济影响。我们在野生动物与家畜的交界处设计了一项调查,以验证这样一个假设,即人类化和野生环境之间的环境有利性差异会调节 WNV 病媒的数量和传播风险。在最近出现 WNF 的地中海大陆地区,我们选择了九个采样点,并为每个采样点分配了三个区域,野生动物与家畜之间的互动呈递减梯度:A1 - 相互影响最大的养马场;A2 - 距离养马场 500-1000 米的中间影响区;A3 - 距离养马场 1-5 公里的完全野生的低影响区。在 2018 年 5 月至 12 月和 2019 年 4 月至 7 月期间,我们以每两周一次的频率估算了 27 个研究地点的蚊虫数量。我们估算了鸟类和哺乳动物的数量,收集了气象信息,并在研究地点范围内描述了蚊子栖息地的特征。之后,我们通过构建负二项广义线性混合模型,研究了库蚊属、滇库蚊(Culex pipiens sensu lato (s.l.) Linnaeus, 1758)(双翅目:库蚊科)和Theobald, 1903库蚊(Culex theileri Theobald, 1903)数量的决定因素。我们发现库蚊的丰度时空分布存在差异,并发现当地环境变化对丰度的重要影响,从而证实了我们的假设。第 4-14 两星期的累积降雨量和取样前两星期的平均气温与 Cx.
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引用次数: 0
Wild birds as hosts of ticks (Acari: Ixodidae) and Anaplasmataceae (Rickettsiales) in the Atlantic rainforest ecoregion, Argentina. 阿根廷大西洋雨林生态区野生鸟类作为蜱虫(蛔虫科:Ixodidae)和立克次体(Anaplasmataceae)的宿主。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-29 DOI: 10.1111/mve.12772
Paula J Vaschalde, Fernando S Flores, Laura B Tauro, Lucas D Monje

This study aimed to assess the presence of Anaplasma, Ehrlichia and Rickettsia species infecting wild birds and their associated ticks in two localities: Moconá Provincial Park (MPP) and Piñalito Provincial Park (PPP), within the Atlantic rainforest ecoregion of Argentina. A total of 142 birds were captured representing 34 species, 28 genera, 15 families and five orders. Ticks were collected from 33 of these birds, comprising 19 species, 14 genera and one order. A total of 59 ticks were collected and identified as Amblyomma calcaratum (13 larvae and four nymphs), Amblyomma longirostre (31 larvae and four nymphs), Amblyomma ovale (one larva and one nymph) and Haemaphysalis leporispalustris (five larvae). All samples were screened for detection of Anaplasma, Ehrlichia and Rickettsia DNA by polymerase chain reaction (PCR) assay. Four bird species tested positive for Anaplasmataceae, with three closely related to Anaplasma and Ehrlichia species previously reported in A. calcaratum adults from a site 15 km away from MPP. The fourth Anaplasmataceae agent was phylogenetically related to Ehrlichia strains reported in Amblyomma triste from the Delta and Parana Islands ecoregion. Additionally, two Rickettsia species were detected and molecularly identified within ticks collected from birds: Rickettsia amblyommatis in A. longirostre larvae and Rickettsia rhipicephali in H. leporispalustris larvae. These findings suggest that birds may play a significant role in the spread of ticks and their associated tick-borne pathogens across diverse biomes.

本研究旨在评估两个地区野生鸟类及其相关蜱虫所感染的阿纳普拉斯马属、埃里希氏菌和立克次体的存在情况:Moconá 省立公园 (MPP) 和 Piñalito 省立公园 (PPP)。共捕获 142 只鸟类,代表 34 种、28 属、15 科和 5 目。从其中 33 只鸟类身上采集到蜱虫,包括 19 种、14 属和 1 目。共采集到 59 只蜱虫,经鉴定分别为:Amblyomma calcaratum(13 只幼虫和 4 只若虫)、Amblyomma longirostre(31 只幼虫和 4 只若虫)、Amblyomma ovale(1 只幼虫和 1 只若虫)和 Haemaphysalis leporispalustris(5 只幼虫)。所有样本均通过聚合酶链式反应(PCR)检测法检测阿纳普拉丝虫、埃立克次体和立克次体的 DNA。四种鸟类的解脲脲原体检测结果呈阳性,其中三种与之前在距离 MPP 15 千米的一个地点报告的钙蝇成虫中的解脲脲原体和埃利希氏原体密切相关。第四个无鞭毛虫科病原体与三角洲和巴拉那群岛生态区报告的Amblyomma triste中的Ehrlichia菌株有系统发育关系。此外,在从鸟类身上采集的蜱虫中检测到两种立克次体,并进行了分子鉴定:在 A. longirostre 幼虫体内发现了 Rickettsia amblyommatis,在 H. leporispalustris 幼虫体内发现了 Rickettsia rhipicephali。这些发现表明,鸟类可能在蜱及其相关蜱传病原体在不同生物群落中的传播中扮演着重要角色。
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引用次数: 0
Culicoides biting midges in urban areas of northern Spain. 西班牙北部城市地区的咬蠓。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-25 DOI: 10.1111/mve.12773
Mikel A González, Aitor Cevidanes, Jesús F Barandika, Ana L García-Pérez

Culicoides biting midges (Diptera: Ceratopogonidae) are known vectors of multiple arboviruses of veterinary and medical importance, as well as avian haemosporidian parasites. Despite their significant role as vectors, the distribution of Culicoides species in non-farmland habitats remains largely unknown. We conducted a study to assess the species community composition, abundance, and host feeding patterns of Culicoides biting midges in urban ecosystems in northern Spain. Light-baited suction traps were operated biweekly for 6 months across six urban landscapes (three cemeteries and three green areas). In total, 181 Culicoides specimens of 18 species were morphologically identified, with the Obsoletus complex being predominant (43.% of the total catches), followed by Culicoides kibunensis (21.5%). Culicoides midges peaked in early June in green areas and in May in cemeteries. Host feeding patterns revealed avian preferences (mainly Turdus spp.) in four Culicoides species. Despite the low overall number of specimens collected, a high diversity of biting midge species was recorded. These findings offer critical insights into Culicoides ecology and their interactions with hosts in urban settings, which are vital for disease surveillance and management strategies.

Culicoides叮咬蠓(双翅目:Ceratopogonidae)是多种具有兽医和医学重要性的虫媒病毒以及鸟类血孢子虫寄生虫的已知载体。尽管Culicoides是重要的传播媒介,但其在非农田栖息地的分布情况在很大程度上仍不为人所知。我们进行了一项研究,以评估西班牙北部城市生态系统中咬蠓的物种群落组成、数量和宿主取食模式。在 6 个月的时间里,我们每两周在 6 个城市景观(3 个墓地和 3 个绿地)中进行一次光诱吸式诱捕。经形态鉴定,共发现 18 个种类的 181 头蚋标本,其中以 Obsoletus 复合体为主(占总捕获量的 43.%),其次是 Culicoides kibunensis(21.5%)。6 月初绿地和 5 月墓地的蠓虫高峰期。寄主取食模式显示,有四种库里科蠓偏爱鸟类(主要是土拨鼠属)。尽管采集到的标本数量较少,但记录到的咬蠓种类却非常多样。这些发现提供了有关城市环境中啮齿类动物生态学及其与宿主相互作用的重要见解,对于疾病监测和管理策略至关重要。
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引用次数: 0
Diversity of lice and flea- and lice-borne pathogens in free-ranging dogs in Uzbekistan. 乌兹别克斯坦自由放养的狗身上的虱子以及跳蚤和虱子传播的病原体的多样性。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-15 DOI: 10.1111/mve.12769
Luciana Cătălina Panait, Alisher Safarov, Georgiana Deak, Angela Monica Ionică, Andrei Daniel Mihalca

Vector-borne diseases pose a significant threat to human and animal health worldwide, with arthropods, including fleas and lice, acting as key vectors for transmitting various pathogens. In Uzbekistan, where millions of domestic dogs coexist with humans, the diversity of vector-borne pathogens carried by ectoparasites remains largely unexplored. This study aimed to investigate the diversity and prevalence of lice and pathogens carried by fleas and lice collected from domestic dogs in Uzbekistan. Ectoparasites were collected from 77 dogs across five regions and subjected to morphological identification and molecular analysis. The 47 lice collected were identified as Trichodectes canis Fabricius, 1780 (Psocodea: Trichodectidae), Linognathus africanus Kellogg and Paine, 1911 (Psocodea: Linognathidae) and Bovicola bovis Linnaeus, 1758 (Phthiraptera: Trichodectidae), alongside with 197 fleas. Notably, L. africanus was detected for the first time on dogs worldwide. Molecular screening detected Rickettsia spp. in 27.9% fleas and 44.7% lice, Dipylidium caninum Linnaeus, 1758 (Cyclophyllidea: Dipylidiidae) in 1.5% of fleas and Bartonella spp. in 0.5% of fleas. Sequencing confirmed Rickettsia asembonensisMaina et al., 2016 (Rickettsiales: Rickettsiaceae), Rickettsia helvetica Beati et al., 1993 (Rickettsiales: Rickettsiaceae), Rickettsia monacensis Simser et al., 2019 (Rickettsiales: Rickettsiaceae), D. caninum and Bartonella clarridgeiae Lawson and Collins, 1996 (Hyphomicrobiales: Bartonellaceae). These findings highlight the potential role of dogs as reservoirs for vector-borne pathogens and emphasises the need for further research to inform effective disease control strategies.

病媒传播的疾病对全球人类和动物健康构成重大威胁,包括跳蚤和虱子在内的节肢动物是传播各种病原体的主要载体。在乌兹别克斯坦,数以百万计的家犬与人类共存,但体外寄生虫携带的病媒传播病原体的多样性在很大程度上仍未得到研究。本研究旨在调查从乌兹别克斯坦家犬身上采集的跳蚤和虱子所携带的虱子和病原体的多样性和流行率。研究人员从五个地区的 77 只家犬身上采集了体外寄生虫,并对其进行了形态鉴定和分子分析。收集到的 47 只虱子被鉴定为 Trichodectes canis Fabricius, 1780(Psocodea: Trichodectidae)、Linognathus africanus Kellogg and Paine, 1911(Psocodea: Linognathidae)和 Bovicola bovis Linnaeus, 1758(Phthiraptera: Trichodectidae),以及 197 只跳蚤。值得注意的是,L. africanus 是首次在全世界的狗身上发现。分子筛查在 27.9% 的跳蚤和 44.7% 的虱子中检测到立克次体,在 1.5% 的跳蚤中检测到犬二联蚤(Dipylidium caninum Linnaeus, 1758)(Cyclophyllidea: Dipylidiidae),在 0.5% 的跳蚤中检测到巴顿氏菌。测序结果证实了 Rickettsia asembonensisMaina 等人,2016 年(立克次体科:立克次体属)、Rickettsia helvetica Beati 等人,1993 年(立克次体科:立克次体属)、Rickettsia monacensis Simser 等人,2019 年(立克次体科:立克次体属)、立克次体科:立克次体属)、D. caninum 和 Bartonella clarridgeiae Lawson 和 Collins,1996 年(表皮微生物科:Bartonellaceae)。这些发现凸显了狗作为病媒传播病原体的潜在贮藏库的作用,并强调了进一步研究的必要性,以便为有效的疾病控制策略提供信息。
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引用次数: 0
Prevalence of and potential risk factors for multiple resistance to acaricides in Rhipicephalus (Boophilus) microplus ticks: A survey in the state of Rio Grande Do Sul, Brazil. Rhipicephalus (Boophilus) microplus蜱对杀螨剂产生多重抗药性的普遍性和潜在风险因素:巴西南里奥格兰德州的一项调查。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-15 DOI: 10.1111/mve.12767
Priscila Teixeira Ferreira, Nathalia de Bem Bidone, Fernando Groff, Patrícia Silva da Silva, Mariana Silveira de Jesus, Debora da Cruz Payao Pellegrini, Rovaina Laureano Doyle, José Reck, Guilherme Klafke

The cattle tick Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Ixodida, Ixodidae) is responsible for significant economic losses in bovine production in tropical and subtropical regions worldwide. Control of this tick predominantly involves the use of chemical acaricides; however, their indiscriminate use has led to the selection of resistant populations. A survey on tick populations was conducted in the state of Rio Grande do Sul, in Brazil, to assess the prevalence of multiple resistance to acaricides in cattle farms. Additionally, questionnaires were administered to identify potential risk factors associated with multiple resistance to acaricides. In total, 176 farms with a bovine population of ≥40 cattle were randomly assigned for tick sampling. The resistance to six acaricidal compounds was investigated by bioassays. A larval packet test was performed for amitraz, chlorpyrifos, cypermethrin, fipronil and ivermectin. Fluazuron was screened using an adult immersion test. Multiple resistance to acaricides (i.e., resistance to three or more compounds) was detected in 173 samples, representing 98% of the total samples. Among these samples, 125 (71%) showed resistance to all six compounds tested. Additionally, we classified the resistance intensity into four levels (I to IV) based on the quartile distribution of the bioassay data. Ten samples (6%) showed high and very high levels (III and IV) of resistance to all six compounds tested. Three variables were significantly associated with multiple resistance to the six acaricides tested: (i) use of injectable acaricides to control ticks, (ii) application of more than five acaricide treatments per year, and (iii) farms with larger herds (≥232 animals). These results regarding widespread resistance and the emergence of multiple resistance to acaricides ticks are alarming and highlight the significant challenge of tick control in southern Brazil.

牛蜱 Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Ixodida, Ixodidae) 是造成全球热带和亚热带地区牛生产重大经济损失的罪魁祸首。对这种蜱虫的控制主要是使用化学杀螨剂;然而,滥用化学杀螨剂导致了抗药性种群的产生。我们在巴西南里奥格兰德州对蜱虫种群进行了调查,以评估牛场对杀螨剂产生多重抗药性的普遍程度。此外,还进行了问卷调查,以确定与杀螨剂多重抗药性相关的潜在风险因素。共随机分配了 176 个牛存栏数≥40 头的农场进行蜱虫采样。通过生物测定调查了六种杀螨化合物的抗药性。对双甲脒、毒死蜱、氯氰菊酯、氟虫腈和伊维菌素进行了幼虫包测试。使用成虫浸泡试验对氟啶脲进行了筛选。在 173 个样本中检测到了对杀螨剂的多重抗药性(即对三种或三种以上化合物产生抗药性),占样本总数的 98%。在这些样本中,125 个样本(71%)对所有六种测试化合物都表现出抗药性。此外,我们还根据生物测定数据的四分位分布将抗药性强度分为四个等级(I 至 IV)。有 10 个样本(6%)对所有六种受测化合物都表现出高度和极高度(III 和 IV)的抗药性。以下三个变量与六种杀螨剂的多重抗药性明显相关:(i) 使用注射杀螨剂控制蜱虫;(ii) 每年使用五次以上杀螨剂;(iii) 畜群较大(≥232 头)的农场。这些关于蜱虫对杀螨剂普遍产生抗药性和多重抗药性的结果令人担忧,凸显了巴西南部在蜱虫控制方面面临的巨大挑战。
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引用次数: 0
Trypanosoma cruzi infection enhances olfactory response in Triatoma pallidipennis Stål (Hemiptera: Triatominae) to compounds potentially useful for insect control. 克鲁兹锥虫感染会增强苍蝇蓟马(半翅目:蓟马科)对可能用于控制昆虫的化合物的嗅觉反应。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-14 DOI: 10.1111/mve.12770
Berenice Jiménez-Santiago, Any Laura Flores-Villegas, Samuel Cruz-Esteban, Margarita Cabrera-Bravo, Conchita Toriello

In Mexico, Triatoma pallidipennis is a major vector of Trypanosoma cruzi, the causative agent of Chagas disease. Current efforts are focused on developing attractants to control these vectors, using volatile substances derived from vertebrate hosts or compounds known to attract hematophagous insects. However, the efficacy of these compounds in attracting parasite-infected triatomines remains to be evaluated. In this study, we assessed the attractant activity of octenol (1-octen-3-ol), nonanal and a mixture of odorants consisting of ammonium hydroxide, lactic acid and hexanoic acid (in a ratio of 1:0.2:0.4 respectively), at concentrations of 1, 10 and 100 ng on the N3, N4 and N5 nymphal stages of T. pallidipennis, both infected and non-infected with T. cruzi. We also evaluated the synergistic effect of the most effective compounds and doses. All experiments were performed in a laboratory using a Y-type glass olfactometer. We found that both infected and non-infected N3 and N4 nymphs were attracted to low doses of octenol, nonanal and the odorant mixture. Particularly noteworthy was the synergistic effect observed between the odorant mixture and nonanal, which significantly increased attraction of T. cruzi-infected individuals. These findings contribute to the development of baited traps utilising these compounds for monitoring triatomines in epidemiological studies or for mass trapping to control these vectors.

在墨西哥,Triatoma pallidipennis 是南美锥虫病的主要病媒。目前的工作重点是利用从脊椎动物宿主身上提取的挥发性物质或已知能吸引食血昆虫的化合物,开发控制这些病媒的引诱剂。然而,这些化合物在吸引受寄生虫感染的三蠹虫方面的功效仍有待评估。在这项研究中,我们评估了辛烯醇(1-辛烯-3-醇)、壬醛以及由氢氧化铵、乳酸和己酸(比例分别为 1:0.2:0.4)组成的混合气味剂(浓度分别为 1、10 和 100 毫微克)对感染和未感染克柔蚊的 N3、N4 和 N5 若虫阶段的吸引活性。我们还评估了最有效化合物和剂量的协同效应。所有实验都是在实验室使用 Y 型玻璃嗅觉仪进行的。我们发现,感染和未感染的 N3 和 N4 若虫都会被低剂量的辛烯醇、壬醛和混合气味剂所吸引。尤其值得注意的是,我们观察到混合气味剂和壬醛之间存在协同效应,可显著提高对受 T. cruzi 感染个体的吸引力。这些发现有助于利用这些化合物开发饵料诱捕器,以便在流行病学研究中监测三蠹虫,或进行大规模诱捕以控制这些病媒。
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引用次数: 0
Inferring the feeding history of the castor bean tick, Ixodes ricinus from lipid and body measurements. 从脂质和身体测量结果推断蓖麻蜱的取食史
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-10 DOI: 10.1111/mve.12768
Munachimso Udobi, Richard Wall

The ability to determine when ticks last fed and assign them to a specific feeding cohort is important in attempts to explain their population dynamics; the biochemical measurement of stored lipid, has been widely used for this purpose. However, when relating feeding history to behaviour or infection status, a non-destructive approach to its assessment would be of value and, to this end, previous studies have attempted to use morphometric indices. Within any instar, the sclerotised scutal components of the body will not vary with increasing starvation while the alloscutal components will, and the resulting ratio should provide a measure of time since feeding. Here, the aim was to determine whether such a morphological ratio (described here as the hunger index) changed predictably with starvation in Ixodes ricinus L. (Ixodida: Ixodidae). For this a cohort of 300 I. ricinus nymphs was collected from the field in February 2021 and starved in a humidified incubator at 15°C and 80% relative humidity (RH). Every 2 weeks, 50 nymphs selected at random were removed and killed by freezing; morphometric measurement was followed by the measurement of lipid using a standard spectrophotometric approach. Both hunger index and stored lipid changed significantly with increasing starvation and were positively correlated with each other. However, the change in morphometric ratio was relatively slight (11%) over 9 weeks and the variation was high. The data suggest therefore that morphological measurements could be used to provide, at best, only broad categorisation of the hunger status of individual I. ricinus ticks in the field.

确定蜱虫最后一次进食的时间并将其归入特定的进食群组对于试图解释其种群动态非常重要;储存脂质的生化测量已被广泛用于此目的。然而,当将摄食史与行为或感染状况联系起来时,一种非破坏性的评估方法将很有价值,为此,以前的研究尝试使用形态计量指数。在任何一个蜕期,身体的硬壳部分都不会随着饥饿程度的增加而变化,而全硬壳部分则会,由此得出的比率应能衡量进食后的时间。本文旨在确定这种形态比率(此处称为饥饿指数)是否会随着蓖麻蜥(Ixodida: Ixodidae)的饥饿而发生可预测的变化。为此,研究人员于 2021 年 2 月从野外采集了 300 只蓖麻若虫,并将其置于 15°C 和 80% 相对湿度(RH)的加湿培养箱中进行饥饿培养。每两周随机抽取 50 只若虫冷冻杀死,然后用标准分光光度法测量脂质。饥饿指数和储存脂质都随着饥饿程度的增加而发生了显著变化,并且两者之间呈正相关。然而,在 9 周的时间里,形态测量比率的变化相对较小(11%),而且变异较大。因此,这些数据表明,形态测量结果充其量只能对蓖麻蜱个体在野外的饥饿状态进行大致分类。
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引用次数: 0
Post-mortem interval determinations using insects collected from illegally hunted and dehorned rhinoceros in the Republic of South Africa from 2014 to 2021. 使用从 2014 年至 2021 年南非共和国非法猎杀和去角犀牛身上采集的昆虫进行死后间隔测定。
IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2024-10-09 DOI: 10.1111/mve.12760
Melanie Pienaar, Ian R Dadour

Wildlife forensic science is a growing research field globally with application in criminal cases of illegal hunting requiring an estimate of time of death based on insect fauna. The techniques and procedures of forensic entomology acquired over the last 40 years, used in legal cases relating to human remains, can be adapted to decomposing wildlife. Research on carrion utilising the rate of development of insect immatures provides a biological clock from which a minimum post-mortem interval (minPMI) can be derived. The following study concerns 19 rhinoceros that were illegally killed and dehorned in the Republic of South Africa between 2014 and 2021. The paper details 74 samples of insect evidence collected from these rhinoceros remains from which an accurate estimate of their PMI was calculated. The specimens comprised 18 species from 12 families belonging to three insect orders. Many Dipteran and Coleopteran species were found on and around each carcass. The species of fly larvae (family Calliphoridae) used in each case to estimate the PMI are as follows: Chrysomya marginalis (Wiedemann) (13 cases), Chrysomya chloropyga (Wiedemann) (2 cases), Chrysomya albiceps (Wiedemann) (1 case) and Chrysomya megacephala (Fabricius) (1 case). Two species of Coleoptera from the family Dermestidae and Silphidae involved Dermestes maculatus (DeGeer) and Thanatophilus micans (Fabricius), respectively, also were involved in one PMI estimation each. The paper highlights opportunities for improving our global understanding of gaps in procedures and training related to wildlife criminal cases.

野生动物法医学是全球不断发展的研究领域,在非法狩猎刑事案件中的应用需要根据昆虫动物群估计死亡时间。法医昆虫学在过去 40 年里获得的技术和程序用于与人类遗骸有关的法律案件,这些技术和程序也可适用于腐烂的野生动物。利用昆虫幼虫的发育速度对腐肉进行的研究提供了一个生物钟,可以从中得出死后最小间隔时间(minPMI)。以下研究涉及 2014 年至 2021 年期间在南非共和国被非法杀害和去角的 19 头犀牛。论文详细介绍了从这些犀牛遗骸中收集到的 74 份昆虫证据样本,并据此计算出其 PMI 的精确估算值。这些标本包括三个昆虫纲 12 个科 18 个种。在每具犀牛尸体及其周围都发现了许多双翅目和鞘翅目昆虫。用于估算 PMI 的苍蝇幼虫(角蝇科)的种类如下:Chrysomya marginalis (Wiedemann) (13 例)、Chrysomya chloropyga (Wiedemann) (2 例)、Chrysomya albiceps (Wiedemann) (1 例) 和 Chrysomya megacephala (Fabricius) (1 例)。Dermestes maculatus (DeGeer) 和 Thanatophilus micans (Fabricius) 两种鞘翅目昆虫也分别参与了一次 PMI 估算。本文强调了在全球范围内提高我们对野生动物刑事案件相关程序和培训差距的认识的机会。
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引用次数: 0
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Medical and Veterinary Entomology
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