Characterization of anthracycline-induced cardiotoxicity by diffusion tensor magnetic resonance imaging

IF 7.5 1区医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Basic Research in Cardiology Pub Date : 2024-03-14 DOI:10.1007/s00395-024-01039-z

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Abstract

Anthracyclines are highly potent anti-cancer drugs, but their clinical use is limited by severe cardiotoxic side effects. The impact of anthracycline-induced cardiotoxicity (AIC) on left ventricular (LV) microarchitecture and diffusion properties remains unknown. This study sought to characterize AIC by cardiovascular magnetic resonance diffusion tensor imaging (DTI). Mice were treated with Doxorubicin (DOX; n = 16) for induction of AIC or saline as corresponding control (n = 15). Cardiac function was assessed via echocardiography at the end of the study period. Whole hearts (n = 8 per group) were scanned ex vivo by high-resolution DTI at 7 T. Results were correlated with histopathology and mass spectrometry imaging. Mice with AIC demonstrated systolic dysfunction (LVEF 52 ± 3% vs. 43 ± 6%, P < 0.001), impaired global longitudinal strain (−19.6 ± 2.0% vs. −16.6 ± 3.0%, P < 0.01), and cardiac atrophy (LV mass index [mg/mm], 4.3 ± 0.1 vs. 3.6 ± 0.2, P < 0.01). Regional sheetlet angles were significantly lower in AIC, whereas helix angle and relative helicity remained unchanged. In AIC, fractional anisotropy was increased (0.12 ± 0.01 vs. 0.14 ± 0.02, P < 0.05). DOX-treated mice displayed higher planar and less spherical anisotropy (C_Planar 0.07 ± 0.01 vs. 0.09 ± 0.01, P < 0.01; C_Spherical 0.89 ± 0.01 vs. 0.87 ± 0.02, P < 0.05). C_Planar and C_Spherical yielded good discriminatory power to distinguish between mice with and without AIC (c-index 0.91 and 0.84, respectively, P for both < 0.05). AIC is associated with regional changes in sheetlet angle but no major abnormalities of global LV microarchitecture. The geometric shape of the diffusion tensor is altered in AIC. DTI may provide a new tool for myocardial characterization in patients with AIC, which warrants future clinical studies to evaluate its diagnostic utility.

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通过弥散张量磁共振成像鉴定蒽环类药物诱发的心脏毒性

摘要蒽环类是强效抗癌药物，但其严重的心脏毒性副作用限制了其临床应用。蒽环类药物诱发的心脏毒性（AIC）对左心室微结构和弥散特性的影响尚不清楚。本研究试图通过心血管磁共振弥散张量成像（DTI）来描述 AIC 的特征。小鼠接受多柔比星（DOX；n = 16）治疗以诱导 AIC，或接受生理盐水作为相应对照（n = 15）。研究结束时通过超声心动图评估心脏功能。研究结果与组织病理学和质谱成像相关联。患有 AIC 的小鼠表现出收缩功能障碍（LVEF 52 ± 3% vs. 43 ± 6%，P < 0.001）、整体纵向应变受损（-19.6 ± 2.0% vs. -16.6 ± 3.0%，P < 0.01）和心脏萎缩（左心室质量指数 [mg/mm], 4.3 ± 0.1 vs. 3.6 ± 0.2，P < 0.01）。在 AIC 中，区域小片角明显降低，而螺旋角和相对螺旋度保持不变。在 AIC 中，分数各向异性增加（0.12 ± 0.01 vs. 0.14 ± 0.02，P < 0.05）。经 DOX 处理的小鼠显示出较高的平面各向异性和较低的球面各向异性（CPlanar 0.07 ± 0.01 vs. 0.09 ± 0.01，P < 0.01；CSpherical 0.89 ± 0.01 vs. 0.87 ± 0.02，P < 0.05）。CPlanar 和 CSpherical 在区分有 AIC 和无 AIC 的小鼠方面具有良好的鉴别力（c 指数分别为 0.91 和 0.84，P 均为 0.05）。AIC 与小片角度的区域性变化有关，但与整体左心室微结构的重大异常无关。扩散张量的几何形状在 AIC 中发生了改变。DTI 可能为 AIC 患者的心肌特征描述提供了一种新工具，值得在未来进行临床研究以评估其诊断效用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Basic Research in Cardiology 医学-心血管系统

CiteScore

16.30

自引率

5.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Basic Research in Cardiology is an international journal for cardiovascular research. It provides a forum for original and review articles related to experimental cardiology that meet its stringent scientific standards. Basic Research in Cardiology regularly receives articles from the fields of - Molecular and Cellular Biology - Biochemistry - Biophysics - Pharmacology - Physiology and Pathology - Clinical Cardiology