Labile organic matter promotes nitrogen retention in natural and constructed gulf coast marshes

Abstract

Coastal marshes mitigate allochthonous nitrogen (N) inputs to adjacent marine habitat; however, their extent is declining rapidly. As a result, marsh restoration and construction have become a major foci of wetland management. Constructed marshes can quickly reach similar plant biomass to natural marshes, but biogeochemical functions like N removal and retention can take decades to reach functional equivalency, often due to lags in organic matter (OM) pools development in newly constructed marshes. We compared denitrification and dissimilatory nitrate reduction to ammonium (DNRA) rates in a 32 year-old constructed marsh and adjacent reference marsh in the Northern Gulf of Mexico. Marsh sediments packed into 3 mm “thin discs” were subjected to three OM quality treatments (no OM addition, labile OM, or recalcitrant OM) and two N treatments (ambient nitrate or elevated nitrate) during a 13 day incubation. We found that OM addition, rather than marsh type or nitrate treatment, was the most important driver of nitrate reduction, increasing both denitrification and DNRA and promoting DNRA over denitrification in both marshes. Fungal and bacterial biomass were higher in the natural marsh across treatments, but recalcitrant OM increased fungal biomass in the constructed marsh, suggesting OM-limitation of fungal growth. We found that constructed marshes are capable of similar denitrification and DNRA as natural marshes after 30 years, and that labile OM addition promotes N retention in both natural and constructed marshes.

Graphical Abstract

Conceptual figure highlighting the findings of this experiment. Under control treatment with no C addition (bottom panel), constructed and natural marshes have similar rates of both DNRA and denitrification. The natural marsh has higher fungal and bacterial biomass, while fungal biomass is not detectable in the constructed marsh. Under labile OM additions (upper left panel), rates of both DNRA and denitrification are increased and DNRA becomes favored over denitrification in both marshes. Recalcitrant OM additions (upper right) increase denitrification, but do not affect DNRA or % denitrification. The addition of recalcitrant OM also increases the detectability of fungal biomass in the constructed marsh.