Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-03-16 DOI:10.1007/s10529-024-03472-5

Qiang Wilson Yan, Ying-Chun Liu, Christa Barrett, Kelly Haake, Daniel Seeler, Oliver May, Ross Zirkle

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Abstract

Objective: Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.

Results: In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of Sphaeroforma arctica that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in S. arctica. Our novel isolate of S. arctica grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.

Conclusion: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.

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在海洋鱼类 Sphaeroforma arctica 的新分离物中积累二十二碳五烯酸（n-3 DPA）。

目的：目前，二十二碳五烯酸（n-3 DPA，C22:5）缺乏一致且高度富集的来源，这项工作报告了天然产生 n-3 DPA 的微生物的分离情况：在这项工作中，我们筛选了培养物中的微生物，目的是分离出一株含有大量 n-3 DPA 的菌株。我们分离出了一株 Sphaeroforma arctica 菌株，该菌株产生的 n-3 DPA 占总脂肪酸的 11%，并且 n-3 DPA 与 DHA/EPA 的比率很高。我们利用显微镜成像和流式细胞仪技术对分离菌株的细胞生长进行了鉴定，证实了之前在北极鞘氨醇中描述的细胞分裂的同源模式。与之前分离和描述的菌株相比，我们新分离出的北极鼠的生长更健壮，产生的 n-3 DPA 明显更多，这表明了所发现菌株的独特性：总之，这项工作报告了第一个分离的 n-3 DPA 生产微生物，并为未来菌株改良和阐明这种低碳-PUFA 对人类营养和健康的生理功能奠定了基础。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.