Cell-free systems for a multi-pronged approach to next-generation therapeutics and diagnostics

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-03-17 DOI:10.1007/s12257-024-00058-w
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Abstract

Effective diagnostics and therapeutics are foundational to modern medical technology. As the drug modality transitions from traditional small-molecule drugs to more intricate protein and nucleic acid-based therapies, there is mounting momentum towards personalized therapeutic approaches. These evolving paradigms challenge conventional biomanufacturing practices, highlighting the need for agile and patient-centric methodologies. Cell-free protein synthesis (CFPS), which facilitates the direct and programmable production of recombinant proteins, emerges as a compelling alternative. It offers rapid, adaptable, and customized protein production capabilities. Furthermore, by leveraging the core molecular mechanisms of gene expression, CFPS is gaining traction in the diagnostics realm, addressing the growing need for sophisticated diagnostic tools. In this review, we survey recent advancements and discuss how CFPS is poised to markedly reshape therapeutic production and diagnostic methods in the foreseeable future.

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多管齐下的新一代治疗和诊断无细胞系统
摘要 有效的诊断和治疗是现代医学技术的基础。随着药物模式从传统的小分子药物过渡到更复杂的基于蛋白质和核酸的疗法,个性化治疗方法的发展势头日益强劲。这些不断发展的模式对传统的生物制造实践提出了挑战,突出了对敏捷和以患者为中心的方法的需求。无细胞蛋白质合成(CFPS)有助于直接和可编程地生产重组蛋白质,是一种引人注目的替代方法。它具有快速、适应性强和定制化的蛋白质生产能力。此外,通过利用基因表达的核心分子机制,CFPS 在诊断领域正获得越来越多的关注,以满足对复杂诊断工具日益增长的需求。在这篇综述中,我们将考察最近的进展,并讨论 CFPS 如何在可预见的未来重塑治疗生产和诊断方法。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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