An overview of DNA degradation and its implications in forensic caseworks

Abstract

DNA (deoxy-ribonucleic acid) is a fundamental molecule housing genetic information crucial for forensic casework. However, its integrity is compromised over time due to degradation, affecting living and deceased organisms. Understanding the factors and mechanisms of DNA degradation is vital across scientific disciplines. DNA degradation is a dynamic process influenced by factors like temperature, humidity, and ultraviolet radiation. The post-mortem interval affects organisms differently, and mechanisms such as hydrolysis, oxidation, and depurination impact DNA structural integrity. In forensic casework, DNA degradation poses challenges because degraded DNA samples can be difficult to analyze. Despite these challenges, DNA degradation has become an invaluable asset in forensic science. Fragmented DNA aids in historical identification and archaeological investigations. Additionally, DNA degradation helps estimate the time since death, assisting investigators in criminal timelines. Forensic experts use degradation patterns to deduce environmental conditions affecting a body, aiding crime scene reconstruction. In criminal investigations, advancements in DNA recovery, like next-generation sequencing, enable the analysis of severely degraded samples, enhancing the identification of suspects and victims. DNA degradation, despite its challenges, is a potent tool in forensic science. Understanding the factors and mechanisms influencing DNA degradation is essential for its effective utilization in criminal casework. With ongoing advancements in forensic techniques and technologies, the reliability and utility of degraded DNA analysis are steadily increasing, helping to bring resolution to complex criminal cases and uncovering hidden clues in forensic investigations.