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Impact of ante-mortem fluoxetine administration on estimation of post-mortem interval and insect activity in rabbit carcasses
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-09-18 DOI: 10.1186/s41935-024-00409-x
Fatema M. Saleh, Assem H. Badawy, Rawda M. Badawy, Asmaa Abdel Rahman, Eslam Adly
Increasing the number of drug-related deaths has affected medico-legal death investigations. Drugs within a corpse have a great impact on the insects’ development rate which in turn will affect the rate of post-mortem decomposition and the estimation of the post-mortem interval. This explains the importance of the application of forensic entomotoxicology, which studies the impact of drugs and toxins on the development and succession patterns of insects. The current study aimed to determine the impact of fluoxetine, one of the selective serotonin reuptake inhibitors (SSRIs), on post-mortem decomposition, insects’ attraction, and its pattern of succession on carcasses. Sixteen healthy male and female Oryctolagus cuniculus rabbits were chosen to be included in this study. They were divided into a treated group of eight rabbits received oral fluoxetine for 28 days and a control group of eight rabbits received oral distilled water for 28 days. After oral administration of 10 mg/kg/day of fluoxetine and distilled water for 28 days, rabbits were sacrificed, and carcasses were transmitted to the roof of Research and Training Centre on Vectors of Diseases at faculty of Sciences, Ain Shams University, for following up the post-mortem decomposition process and insect’s attraction to carcasses for 60 days. Carcasses were put in two outdoor sites that differ in temperature to assess the effect of temperature on decomposition process. Fluoxetine administration has accelerated the rate of post-mortem decomposition in the treated carcasses by 3–9 days compared to the control ones and affected the numbers and species of attracted insects, while it had no effect on the insects’ succession patterns. Exposure of carcasses to direct sunlight has accelerated the rate of decomposition in comparison to that of carcasses put in shade in the overall period of decomposition by about 14 to 16 days. Fluoxetine has an important and effective role in post-mortem decomposition and estimation of post-mortem interval (PMI) and has a great impact on attracted insects to the treated carcasses. Temperature has a great effect on the rate of decomposition of carcasses. Higher temperature accelerates the rate of post-mortem decomposition.
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引用次数: 0
Particulate matter in necropsy activities: experience from a health operators’ exposure monitoring campaign 尸体解剖活动中的微粒物质:卫生操作人员暴露监测活动的经验
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-09-06 DOI: 10.1186/s41935-024-00406-0
Matteo Scopetti, Nicola Di Fazio, Martina Padovano, Giuseppe Delogu, Donato Morena, Paola Frati, Carmela Protano, Matteo Vitali, Vittorio Fineschi
Operators in the obituary and necropsy sectors are exposed to various environmental hazards during specific tasks. Despite this exposure, occupational risks have often been underestimated, resulting in a lack of substantial evidence. The primary objectives of this study were to identify sources of chemical risk, establish procedures for monitoring and quantifying exposure during necropsy activities, and recommend adjustments to regulatory guidelines to protect the health of the operators. The study was conducted at the Legal Medicine Unit of the Umberto I General Hospital in Rome, focusing on the quantitative measurement of particulate matter (PM) exposure among at-risk operators during necropsy activities. Environmental levels of total suspended particles, PM10, PM4, PM2.5, and PM1 were assessed by evaluating the average, minimum, and maximum instantaneous indoor concentrations using an airborne analyzer. The monitoring activities revealed that the PM concentrations were significantly lower than the recognized reference values. However, bone sawing, body removal, and cleaning were identified as high-risk maneuvers for dust suspension. Our study highlighted specific risks associated with necropsy activities, particularly concerning timing and certain maneuvers. These results may lead to interventions for improving current prevention procedures, implementing good practices, and developing specific guidelines to enhance operator safety.
讣告和尸体解剖部门的操作人员在执行特定任务时会接触到各种环境危害。尽管存在这种接触,但职业风险往往被低估,导致缺乏实质性证据。这项研究的主要目的是确定化学风险的来源,建立监测和量化尸体解剖活动中接触风险的程序,并建议调整监管指南以保护操作人员的健康。这项研究在罗马翁贝托一世综合医院法医部进行,重点是对尸体解剖活动中高危操作人员的颗粒物(PM)暴露进行定量测量。通过使用空气分析仪评估室内瞬时浓度的平均值、最小值和最大值,对总悬浮颗粒物、PM10、PM4、PM2.5 和 PM1 的环境水平进行了评估。监测活动显示,可吸入颗粒物的浓度明显低于公认的参考值。不过,锯骨、移除尸体和清洗被确定为粉尘悬浮的高风险操作。我们的研究强调了与尸体解剖活动相关的特定风险,尤其是在时间安排和某些操作方面。这些结果可能会导致采取干预措施,以改进当前的预防程序、实施良好操作规范和制定具体准则,从而提高操作人员的安全。
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引用次数: 0
Does the distribution of Wormian bone frequencies across different world regions reflect genetic affinity between populations? 世界不同地区的沃姆人骨骼频率分布是否反映了不同种群之间的遗传亲缘关系?
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-09-06 DOI: 10.1186/s41935-024-00405-1
Alla Movsesian
Wormian, or sutural bones, are additional, irregularly shaped bone fragments that can occur within cranial sutures. These bones may arise due to various factors, including mechanical pressure on skull bones during early ontogenetic stages, such as during artificial cranial deformations, or due to genetic and environmental influences. This study investigates the potential genetic basis of sutural bones by comparing their frequencies across diverse global regions. It analyzed 33 craniological series, encompassing 2059 crania, to assess the frequency of sutural bones in the coronal, squamous, lambdoid, and occipitomastoid sutures among skeletal populations from regions including Aboriginal Australia, Melanesia, Southeast Asia, Siberia, Europe, and Native America. Biological distances between populations were calculated using Smith’s mean measure of divergence (MMD), with results visualized through multidimensional scaling. The analysis identified distinct clusters of Caucasian and Siberian populations. Siberian aboriginal populations are compactly grouped, consistent with mtDNA data indicating genetic roots dating back to the Neolithic inhabitants of the Lake Baikal region. Further, differentiation within these populations is linked to the founder effect and gene flow. Notably, genetically related groups like the Inuit and Chukchi of Chukotka differ from other Siberian groups. In contrast, southern Siberian populations, such as the Buryats and Mongols, are closely positioned, aligning with genetic data. The differentiation between Southeast Asian and African regions was subtler, with their clusters largely overlapping. Yet, genetic links between populations were observed in some cases. Thus, Australians, Melanesians, and Papua New Guineans were located close to each other on the multidimensional scaling map, as were two African populations. The findings tentatively suggest a potential genetic component in the expression of Wormian bones, although this hypothesis requires further empirical support, particularly through genetic studies. While genetic factors may influence the expression of Wormian bones, environmental conditions and pathological processes also play significant roles. It can be suggested that Wormian bones could potentially serve as an additional tool in kinship analysis within burials; however, their utility significantly depends on the extent of their genetic influence. If future genetic studies confirm a substantial genetic component and its dominance over environmental factors, the use of these bones in anthropological and forensic analyses would receive additional validation.
瘤骨或缝骨是颅骨缝内可能出现的额外的、形状不规则的骨碎片。这些骨骼可能是由于各种因素造成的,包括颅骨在早期发育阶段受到的机械压力(如人工颅骨变形),或由于遗传和环境影响。本研究通过比较缝骨在全球不同地区的出现频率,研究缝骨的潜在遗传基础。研究分析了 33 个颅骨系列,包括 2059 个颅骨,以评估来自澳大利亚土著、美拉尼西亚、东南亚、西伯利亚、欧洲和美洲原住民等地区的骨骼人群中冠状缝、鳞状缝、λ状缝和枕状缝的缝骨频率。使用史密斯的平均差异度量(MMD)计算了不同种群之间的生物学距离,并通过多维缩放将结果可视化。分析结果表明,高加索人和西伯利亚人有明显的种群集群。西伯利亚原住民聚居在一起,与 mtDNA 数据显示的贝加尔湖地区新石器时代居民的遗传根源相一致。此外,这些人群内部的分化与始祖效应和基因流动有关。值得注意的是,楚科奇的因纽特人和楚科奇人等基因相关群体与其他西伯利亚群体不同。相比之下,布里亚特人和蒙古人等西伯利亚南部人群的位置很接近,这与遗传数据一致。东南亚和非洲地区之间的分化更为微妙,它们的聚类在很大程度上是重叠的。然而,在某些情况下,人群之间也存在遗传联系。因此,澳大利亚人、美拉尼西亚人和巴布亚新几内亚人在多维尺度图上的位置很接近,两个非洲种群也是如此。研究结果初步表明,沃米安骨的表达可能与遗传因素有关,但这一假设还需要进一步的实证支持,特别是通过遗传研究。虽然遗传因素可能会影响 Wormian 骨的表达,但环境条件和病理过程也起着重要作用。可以说,虫骨有可能成为墓葬中亲缘关系分析的另一种工具;不过,其效用在很大程度上取决于其遗传影响的程度。如果未来的遗传学研究证实了大量的遗传因素,而且遗传因素在环境因素中占主导地位,那么这些骸骨在人类学和法医学分析中的应用将得到进一步的验证。
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引用次数: 0
Knowledge and attitude of university nursing students towards forensic nursing and their influencing factors: a mixed-methods study 大学护理专业学生对法医护理的认识和态度及其影响因素:一项混合方法研究
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-08-17 DOI: 10.1186/s41935-024-00404-2
Ebtsam Aly Abou Hashish
Forensic nursing, which merges healthcare and legal expertise to address trauma and violence, is vital yet often underrepresented in nursing education. Many nursing students lack knowledge in this field, underscoring the need for enhanced education to improve patient care and legal outcomes. This study aimed to assess university nursing students’ knowledge and attitudes towards forensic nursing, as well as identify factors influencing their knowledge. A mixed-methods design was conducted at a Saudi nursing college with a convenience sample of 250 students. The data were collected using a structured questionnaire comprising two parts. The quantitative part included demographic and academic characteristics, knowledge and opinions on forensic nursing, knowledge of forensic evidence, and attitudes towards forensic nursing. The qualitative part consisted of four open-ended questions about factors affecting forensic knowledge. Data analysis involved inferential statistics and content analysis. The study revealed that 80.4% of nursing students lacked prior knowledge of forensic nursing, and only 59.76% had some awareness of forensic evidence. A strong positive relationship was found between attitudes towards forensic nursing and knowledge of forensic evidence (r = 0.817, p < 0.001), indicating that positive attitudes could predict 66.8% of the variance in forensic evidence knowledge. Key barriers included lack of support from upper management, insufficient resources, and concerns about faculty qualifications. Students recommended integrating forensic nursing into the curriculum, offering specialized programs, and promoting research and social media awareness campaigns. These findings underscore significant gaps in knowledge and misconceptions about forensic nursing among participants, emphasizing the critical need for education and awareness in this field. The study highlights the potential implications for integrating forensic nursing concepts into undergraduate curricula, enhancing faculty training, and utilizing diverse teaching modalities. Addressing these gaps will not only improve future nurses’ competency in forensic nursing, but also contribute to better patient care.
法医护理融合了医疗保健和法律专业知识以解决创伤和暴力问题,其重要性不言而喻,但在护理教育中却往往代表性不足。许多护理专业的学生缺乏对这一领域的了解,因此需要加强教育以改善患者护理和法律效果。本研究旨在评估大学护理专业学生对法医护理的知识和态度,并确定影响其知识的因素。研究采用混合方法设计,在沙特一所护理学院进行,方便抽样调查了 250 名学生。数据收集采用了结构化问卷,包括两个部分。定量部分包括人口和学术特征、对法医护理的了解和看法、法医证据知识以及对法医护理的态度。定性部分包括四个开放式问题,涉及影响法医知识的因素。数据分析包括推理统计和内容分析。研究显示,80.4%的护理专业学生缺乏法医护理知识,只有 59.76%的学生对法医证据有一定的了解。研究发现,对法医护理的态度与法医证据知识之间存在很强的正相关关系(r = 0.817,p < 0.001),表明积极的态度可以预测法医证据知识中 66.8% 的差异。主要障碍包括缺乏上层管理人员的支持、资源不足以及对教师资格的担忧。学生们建议将法医护理纳入课程、开设专业课程、促进研究和社交媒体宣传活动。这些发现凸显了参与者在法医护理知识方面的巨大差距和误解,强调了在这一领域开展教育和提高认识的迫切需要。研究强调了将法医护理概念纳入本科课程、加强师资培训和利用多样化教学模式的潜在意义。缩小这些差距不仅能提高未来护士在法医护理方面的能力,还有助于更好地护理病人。
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引用次数: 0
Age estimation from the sternal end of left fourth rib in the Nepalese population 从尼泊尔人左侧第四根肋骨胸骨末端推算年龄
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-07-27 DOI: 10.1186/s41935-024-00403-3
Rijen Shrestha, Kewal Krishan, Tanuj Kanchan
Forensic anthropological examinations help in identification of unidentified human remains. This study aims to establish population-specific standards for age estimation from the sternal ends of left fourth ribs in the Nepalese population. A quantitative, observational study was conducted on 387 Nepalese deceased (106 females, 281 males) between November 2021 and March 2023. Left fourth ribs were collected, macerated and examined for the study. The variables examined included pit depth, pit shape, rim and wall configurations and rib phase. Pit depth significantly predicted age, β = 0.642, t(385) = 16.42, p < 0.001. Pit depth also explained a significant proportion of variance in age, R2 = 0.41, F(1, 385) = 269.54, p < 0.001. The age estimates from pit depth stage were 7–36 (stage 0), 7–49 (stage 1), 16–51 (stage 2), 10–58 (stage 3), 22–70 (stage 4) and 32–81 (stage 5). Age estimates for pit shape were 17–20 (stage 0), 13–48 (stage 2), 15–55 (stage 3), 24–68 (stage 4) and 39–82 (stage 5). Age estimates for rim and wall configurations were 17–20 (stage 0), 13–47 (stage 2), 17–53 (stage 3), 24–68 (stage 4) and 40–82 (stage 5). Similarly, age estimates for rib phase were 17–20 (phase 0), 18–20 (phase 1), 13–46 (phase 2), 18–50 (phase 3), 23–60 (phase 4), 32–71 (phase 5), 50–76 (phase 6), 44–81 (phase 7) and 62–85 (phase 8). The study also examined the use of transitional analysis to develop posterior probability distributions for estimation of age using rib phases. This study found significant differences in the age estimates from previous studies and shows the importance of developing population-specific models for use in forensic anthropology.
法医人类学检查有助于识别身份不明的人类遗骸。本研究旨在建立尼泊尔人口中根据左侧第四根肋骨胸骨末端推算年龄的特定人群标准。在 2021 年 11 月至 2023 年 3 月期间,对 387 名尼泊尔逝者(106 名女性,281 名男性)进行了定量观察研究。研究收集、浸渍和检查了左侧第四根肋骨。研究变量包括凹坑深度、凹坑形状、边缘和壁构造以及肋骨相位。凹坑深度可明显预测年龄,β = 0.642,t(385) = 16.42,p < 0.001。坑深也能解释年龄的很大一部分变异,R2 = 0.41, F(1, 385) = 269.54, p < 0.001。坑深阶段的年龄估计值分别为 7-36(0 阶段)、7-49(1 阶段)、16-51(2 阶段)、10-58(3 阶段)、22-70(4 阶段)和 32-81(5 阶段)。坑形的年代估计值为 17-20(第 0 阶段)、13-48(第 2 阶段)、15-55(第 3 阶段)、24-68(第 4 阶段)和 39-82(第 5 阶段)。坑缘和坑壁结构的年代估计分别为 17-20(0 期)、13-47(2 期)、17-53(3 期)、24-68(4 期)和 40-82(5 期)。同样,肋骨阶段的年龄估计值为 17-20(0 阶段)、18-20(1 阶段)、13-46(2 阶段)、18-50(3 阶段)、23-60(4 阶段)、32-71(5 阶段)、50-76(6 阶段)、44-81(7 阶段)和 62-85(8 阶段)。该研究还探讨了使用过渡分析法来建立后验概率分布,以利用肋骨阶段来估计年龄。这项研究发现年龄估计值与以前的研究有很大不同,这表明了开发特定人群模型用于法医人类学的重要性。
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引用次数: 0
Sex estimation using Magnetic Resonance Imaging measurements of hand and foot in Egyptian population 利用 Magnetic Resonance Imaging 测量埃及人手脚的性别估计值
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-07-25 DOI: 10.1186/s41935-024-00393-2
Marwa Abd El-Moniem Amer, Marwa Elsayed Abd Elhamed, Lamiaa Lotfy Elhawy, Amany Mohammed AbdAllah, Nanies Sameeh Mohammad, Manar A. Bessar, Mohamed Nabil Soliman Elgebely, Dena Mohamed Naguib Abdel Moawed
The identification of skeletal remains begins with a sex evaluation since other biological profile elements, such as size and age, are sex-specific. The pelvis is the favored element for sex assessment since it is very sexually dimorphic. Delivering hand and foot bones sex estimate criteria for Egyptian population identification was the goal of this work. Eighty-two Egyptian adult subjects (41 males and 41 females) aged ≥ 21 years old were subjected to MRI scan on the right hand and foot to assess 9 measurements: hand length, hand width, four measurements of middle metacarpal bone (length, breadth, head breadth and base breadth), first metatarsal bone length, first metatarsal bone width and (first metatarsal bone length x first metatarsal bone width). All the measured parameters in the right hand and foot were higher in males than females. First metatarsal bone length x first metatarsal bone width in the foot and middle metacarpal bone breadth in the hand were the most sexually dimorphic parameters, with accuracy of 86.6 percent and 84.1 percent, respectively. In stepwise discriminant analysis, three of nine measurements were selected: First metatarsal bone length x first metatarsal bone width, middle metacarpal bone breadth and hand width for the sex prediction equation in the Egyptian population, with a cross-validated sex classification accuracy of 90.2%. Sex can be assessed from hand and foot metric parameters measured by MRI with high accuracy.
骨骼遗骸的鉴定首先要进行性别评估,因为其他生物特征要素,如体型和年龄,都有性别特异性。骨盆是性别评估的首选要素,因为它具有很强的性别二态性。为埃及人口鉴定提供手骨和脚骨性别估计标准是这项工作的目标。82 名年龄≥ 21 岁的埃及成年受试者(41 名男性和 41 名女性)接受了右手和右脚的核磁共振成像扫描,以评估 9 项测量指标:手长、手宽、中掌骨的四项测量指标(长、宽、头宽和基底宽)、第一跖骨长、第一跖骨宽和(第一跖骨长 x 第一跖骨宽)。男性右手和右脚的所有测量参数均高于女性。足部的第一跖骨长 x 第一跖骨宽和手部的中掌骨宽是最具性别二态性的参数,准确率分别为 86.6% 和 84.1%。在逐步判别分析中,九个测量值中有三个被选中:第一跖骨长 x 第一跖骨宽、中掌骨宽和手宽被选中用于埃及人群的性别预测方程,交叉验证的性别分类准确率为 90.2%。通过核磁共振成像测量的手脚度量参数来评估性别的准确率很高。
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引用次数: 0
Medicolegal assessment of reported female sexual assaults in the Kingdom of Bahrain: record-based descriptive study 对巴林王国报告的女性性侵犯案件进行法医评估:基于记录的描述性研究
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-07-09 DOI: 10.1186/s41935-024-00402-4
Fatema Abdul-Rahman Alfadhel, Mohammed Nour El-Din Fouda, Mona Mohamed Awny
Sexual assault is an important social issue that influences women’s lives. Despite a significant increase in 2018, there is no reliable information available in the Kingdom of Bahrain about female sexual assaults. The aim of this study is to investigate the pattern of reported sexual assaults among living female victims in the Kingdom of Bahrain. All medicolegal reports of sexual assaults in living female victims from 2016 to 2020 were included. Reported cases were studied for sociodemographic data of victims, assailant data, assault circumstances, and medicolegal findings and procedures. The prevalence of female sexual assault allegations was 7%. During the studied period, there were 272 cases of sexual assault overall. Most cases were in the age group 18–30 years (41.2%). In 58.1% of assaults, the assailant was the boyfriend or partner of the victim. The medicolegal examination took place within 2 days after assault in only 32%. Forensic examination of sexual assault victims revealed extragenital injuries in only 30.9% of cases. Sexual assaults involving mixed penetration occurred in 66.9% of victims, followed by vaginal penetration (30.1%), then anal penetration (2.9%). The final medicolegal opinion confirmed positive assault in 38.6% of allegations and negative assault in 32.4% of allegations, while allegations that have no firm medicolegal conclusion were 29%. Despite the efforts made in Bahrain, there is an underestimated problem of sexual violence against females which needs a robust multilevel strategy including the roles of education, law, and non-governmental institutions.
性侵犯是影响妇女生活的一个重要社会问题。尽管在 2018 年出现了大幅增长,但巴林王国没有关于女性性侵犯的可靠信息。本研究旨在调查巴林王国在世女性受害者所报告的性侵犯模式。研究纳入了 2016 年至 2020 年间所有关于在世女性受害者遭受性侵犯的法医报告。研究了报告案件中受害者的社会人口数据、攻击者数据、攻击情况以及法医结论和程序。女性性侵犯指控的发生率为 7%。在研究期间,共发生 272 起性侵犯案件。大多数案件发生在 18-30 岁年龄组(41.2%)。在 58.1%的性侵犯案件中,侵犯者是受害者的男友或伴侣。仅有 32% 的案件在袭击发生后 2 天内进行了法医检查。对性侵犯受害者进行的法医检查发现,只有 30.9%的案件有生殖器外损伤。66.9%的性侵犯受害者遭受了混合插入,其次是阴道插入(30.1%),然后是肛门插入(2.9%)。38.6% 的指控经最终法医意见确认为正面侵犯,32.4% 的指控为负面侵犯,而 29% 的指控没有明确的法医结论。尽管巴林做出了努力,但针对女性的性暴力问题仍被低估,需要采取强有力的多层次战略,包括发挥教育、法律和非政府机构的作用。
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引用次数: 0
Is it time for a guideline on the use of immunohistochemistry in forensic pathology? 现在是制定法医病理学免疫组化使用指南的时候了吗?
IF 1 Q3 MEDICINE, LEGAL Pub Date : 2024-06-17 DOI: 10.1186/s41935-024-00401-5
Massimo Lancia, Mauro Bacci

In the field of forensic trauma pathology, immunohistochemistry (IHC) and specialized staining techniques are crucial to estimate the age of the wound and to identify complications stemming from the trauma. In addition, they are essential to detect unique cases, including those of asphyxia and severe head trauma (Chen et al. 2021).

The use of IHC is also promising for tackling fundamental questions in forensic medicine, such as those related to the age-dating of skin injuries, surpassing the limitations of older techniques based on histochemical techniques (Tomassini et al. 2022; Tomassini et al. 2024).

Despite its promise, the rate of IHC staining undergoes significant variations over time after death, due to postmortem involutional phenomena affecting tissues and altering their antigen-antibody affinity. This has been demonstrated by studies conducted on populations consisting of medico-legal autopsies (Lesnikova et al. 2018).

This is indeed critical, as the practical application of IHC is documented in the literature and proposed in a variety of very complex and sensitive procedural contexts. For example, in homicide cases, where a positive immunohistochemistry result can provide substantial evidence to establish trial truth (Tong et al 2017).

Hence, the use of IHC in forensic pathology is an extremely sensitive issue, as the literature in this field has advocated the application of these techniques in a vast variety of cases related to both natural and violent deaths.

Often, these recommendations are based on studies conducted on relatively small cadaveric populations and not always through adequate standardization of the study population. This is surprising considering that, in courts of law, forensic pathologist consultants frequently invoke the negativity or positivity of a particular IHC staining; this raises the question—assuming the character of evidence before a court -: is that technique truly scientifically validated? Are there clear indications from guidelines and/or protocols regarding the use of one technique over another, or under what conditions the use of either technique should be excluded?

At this juncture, there is a compelling need for the issuance of clear guidance indicating when these techniques may be employed and under what conditions. Specifically addressing the time since death within which the technique is valid, its state of preservation, and the susceptibility of the IHC technique to post-mortem modifications. This is necessary to ensure the proper utilization of these techniques and to provide a fruitful incentive for their study and further exploration in various forensic-medical issues that remain largely unresolved.

Not applicable.

IHC:

Immunohistochemistry

  • Chen XS, Chu J, Yang LJ, Wang T, Tao LY (2021)

在法医创伤病理学领域,免疫组化(IHC)和特殊染色技术对于估计伤口年龄和识别创伤并发症至关重要。此外,它们对于检测特殊病例,包括窒息和严重头部创伤病例也至关重要(Chen et al.尽管 IHC 染色技术前景广阔,但由于死后组织的退缩现象会影响组织并改变其抗原-抗体亲和力,IHC 染色率会随着死亡时间的推移而发生显著变化。这一点已在对医学法律尸检人群进行的研究中得到证实(Lesnikova 等人,2018 年)。这一点确实很关键,因为 IHC 的实际应用已在文献中有所记载,并被提议用于各种非常复杂和敏感的程序环境中。例如,在凶杀案中,免疫组化阳性结果可为确定审判真相提供大量证据(Tong 等人,2017 年)。因此,IHC 在法医病理学中的应用是一个极其敏感的问题,因为该领域的文献主张在与自然死亡和暴力死亡有关的大量案件中应用这些技术。通常情况下,这些建议是基于在相对较小的尸体群体中进行的研究,而且并不总是通过对研究群体进行充分的标准化。令人惊讶的是,在法庭上,法医病理学家顾问经常援引特定 IHC 染色的阴性或阳性;这就提出了一个问题--假定其具有法庭证据的性质--该技术是否真正经过科学验证?指南和/或规程中是否有明确说明使用一种技术优于另一种技术,或者在什么情况下应排除使用其中任何一种技术?目前,亟需发布明确的指南,说明何时可以使用这些技术,以及在什么情况下可以使用。特别是要解决自死亡时间起该技术的有效期、其保存状态以及 IHC 技术对死后改变的易感性等问题。这对于确保正确使用这些技术,并为研究和进一步探索在很大程度上仍未解决的各种法医问题提供富有成效的激励是必要的。Fa Yi Xue Za Zhi 37(5):666-72. https://doi.org/10.12116/j.issn.1004-5619.2020.400817Article PubMed Google Scholar Lesnikova I, Schreckenbach MN, Kristensen MP, Papanikolaou LL, Hamilton-Dutoit S (2018) Usability of immunohistochemistry in forensic samples with varying decomposition.https://doi.org/10.1097/PAF.0000000000000408Article PubMed Google Scholar Tomassini L, Manta AM, Naso I, Adelini V, Paolini D, Petrasso PEY et al (2022) Rust stains' response to environmental stresses: an experimental study on porcine skin.https://doi.org/10.1016/j.jflm.2022.102402Article PubMed Google Scholar Tomassini L, Lancia M, Scendoni R, Manta AM, Fruttini D, Terribile E et al (2024) Dating skin lesions of forensic interest by immunohistochemistry and immunofluorescence techniques: a scoping literature review.Diagnostics 14(2):168. https://doi.org/10.3390/diagnostics14020168Article CAS PubMed PubMed Central Google Scholar Tong F, Wu R, Huang W, Yang Y, Zhang L, Zhang B et al (2017) Forensic aspects of homicides by insulin overdose.Forensic Sci Int 278:9-15. https://doi.org/10.1016/j.forsciint.2017.06.015文章CAS PubMed Google Scholar 下载参考文献不适用。本研究未从公共、商业或非营利部门的资助机构获得任何特定资助。作者和单位佩鲁贾大学医学和外科系法医学、法医学和运动医学科,Piazza Lucio Severi, 06132, Perugia, ItalyMassimo Lancia &amp; Mauro Bacci作者Massimo Lancia查看作者发表的文章您也可以在PubMed Google Scholar中搜索该作者Mauro Bacci查看作者发表的文章您也可以在PubMed Google Scholar中搜索该作者ContributionsM.M.B.和M.L.参与了这项工作的构思。M.L.和M.B.参与了原稿的编写。M.L. 对手稿进行了审阅和编辑。M.B. 提供指导。
{"title":"Is it time for a guideline on the use of immunohistochemistry in forensic pathology?","authors":"Massimo Lancia, Mauro Bacci","doi":"10.1186/s41935-024-00401-5","DOIUrl":"https://doi.org/10.1186/s41935-024-00401-5","url":null,"abstract":"<p>In the field of forensic trauma pathology, immunohistochemistry (IHC) and specialized staining techniques are crucial to estimate the age of the wound and to identify complications stemming from the trauma. In addition, they are essential to detect unique cases, including those of asphyxia and severe head trauma (Chen et al. 2021).</p><p>The use of IHC is also promising for tackling fundamental questions in forensic medicine, such as those related to the age-dating of skin injuries, surpassing the limitations of older techniques based on histochemical techniques (Tomassini et al. 2022; Tomassini et al. 2024).</p><p>Despite its promise, the rate of IHC staining undergoes significant variations over time after death, due to postmortem involutional phenomena affecting tissues and altering their antigen-antibody affinity. This has been demonstrated by studies conducted on populations consisting of medico-legal autopsies (Lesnikova et al. 2018).</p><p>This is indeed critical, as the practical application of IHC is documented in the literature and proposed in a variety of very complex and sensitive procedural contexts. For example, in homicide cases, where a positive immunohistochemistry result can provide substantial evidence to establish trial truth (Tong et al 2017).</p><p>Hence, the use of IHC in forensic pathology is an extremely sensitive issue, as the literature in this field has advocated the application of these techniques in a vast variety of cases related to both natural and violent deaths.</p><p>Often, these recommendations are based on studies conducted on relatively small cadaveric populations and not always through adequate standardization of the study population. This is surprising considering that, in courts of law, forensic pathologist consultants frequently invoke the negativity or positivity of a particular IHC staining; this raises the question—assuming the character of evidence before a court -: is that technique truly scientifically validated? Are there clear indications from guidelines and/or protocols regarding the use of one technique over another, or under what conditions the use of either technique should be excluded?</p><p>At this juncture, there is a compelling need for the issuance of clear guidance indicating when these techniques may be employed and under what conditions. Specifically addressing the time since death within which the technique is valid, its state of preservation, and the susceptibility of the IHC technique to post-mortem modifications. This is necessary to ensure the proper utilization of these techniques and to provide a fruitful incentive for their study and further exploration in various forensic-medical issues that remain largely unresolved.</p><p>Not applicable.</p><dl><dt style=\"min-width:50px;\"><dfn>IHC:</dfn></dt><dd>\u0000<p>Immunohistochemistry</p>\u0000</dd></dl><ul data-track-component=\"outbound reference\" data-track-context=\"references section\"><li><p>Chen XS, Chu J, Yang LJ, Wang T, Tao LY (2021) ","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methamphetamine and carbon monoxide co-toxicity: an unusual case report and literature review 甲基苯丙胺和一氧化碳共同中毒:一个不寻常的病例报告和文献综述
IF 1 Q2 Social Sciences Pub Date : 2024-06-12 DOI: 10.1186/s41935-024-00399-w
Uğur Ata, Cemil Çelik, N. E. Saka
{"title":"Methamphetamine and carbon monoxide co-toxicity: an unusual case report and literature review","authors":"Uğur Ata, Cemil Çelik, N. E. Saka","doi":"10.1186/s41935-024-00399-w","DOIUrl":"https://doi.org/10.1186/s41935-024-00399-w","url":null,"abstract":"","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141353871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sex and age estimation with corneal topography parameters by using machine learning algorithms and artificial neural networks 利用机器学习算法和人工神经网络,通过角膜地形图参数估计性别和年龄
IF 1 Q2 Social Sciences Pub Date : 2024-06-11 DOI: 10.1186/s41935-024-00400-6
Nesibe Yilmaz, Y. Secgin, Kadir Mercan
{"title":"Sex and age estimation with corneal topography parameters by using machine learning algorithms and artificial neural networks","authors":"Nesibe Yilmaz, Y. Secgin, Kadir Mercan","doi":"10.1186/s41935-024-00400-6","DOIUrl":"https://doi.org/10.1186/s41935-024-00400-6","url":null,"abstract":"","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141358264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Egyptian journal of forensic sciences
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