Electrochemical behavior and determination of pyridoxine at chemically modified carbon paste electrode by electropolymerization

Abstract

Pyridoxine and riboflavin can be determined individually or together using a carbon paste electrode modified by electropolymerization method with aspartic acid (PAMCPE). Using scanning electron microscopy, electrochemical impedance spectroscopy, differential pulse voltammetry, and cyclic voltammetry, the electrochemical behavior of pyridoxine was examined. The results of the studies showed that PAMCPE facilitates pyridoxine oxidation. The technique made it possible to determine pyridoxine and riboflavin simultaneously using cyclic voltammetry. The pH study showed that the good current response was observed at 6.5 pH; hence, it was chosen as the optimum for the analysis. The electrochemical reaction of pyridoxine is adsorption controlled with two-electron transfer process. Pyridoxine has a linear working range of 2.5–8.0 μM, with detection limit of 2.95 μM and limit of quantification of 9.85 μM. The developed modified electrode exhibits a number of benefits, including an easy preparation process, good stability, easy surface modification procedure, excellent reproducibility and repeatability. The practical applicability of PAMCPE for the detection of pyridoxine in pharmaceutical sample shows good sensitivity and selectivity.

Graphical abstract