Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus)

Antonie van Leeuwenhoek Pub Date : 2024-03-20 DOI:10.1007/s10482-024-01959-9

Alejandra de Jesús Chavarín-Meza, Bruno Gómez-Gil, Adrián González-Castillo

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Abstract

The family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, Vibrio panuliri, V. ponticus, V. rhodolitus, and V. taketomensis. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (Lutjanus guttatus), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus Vibrio and they belong to the Ponticus clade. Then, a phylogenomic analysis was performed with four type strains and four reference strains isolated from marine organisms and aquatic environments. Multilocus Sequence Analysis (MLSA) of 139 single-copy genes showed that CAIM 703 and CAIM 1902 belong to V. panuliri. The 16S rRNA sequence similarity value between CAIM 703 and CAIM 1902 was 99.61%. The Ponticus clade species showed Average Nucleotide Identity (ANI) values between 78 to 80% against the two strains for ANIb, except V. panuliri LBS2^T (99% and 100% similarity). Finally, this analysis represents the first phylogenomic analysis of the Ponticus clade where V. panuliri strains are reported from Mexico.

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Ponticus 支系的系统进化分析：从斑点玫瑰鲷（Lutjanus guttatus）中分离出的菌株

弧菌科根据其系统发育关系分为许多支系。Ponticus 支系是其支系之一，由 4 个种组成，即 Panuliri 弧菌、V. ponticus 弧菌、V. rhodolitus 弧菌和 V. taketomensis 弧菌。从患病的斑点玫瑰鲷（Lutjanus guttatus）外部病变中分离出两株菌株（CAIM 703 和 CAIM 1902），对它们进行分析以确定其分类学位置，根据 16S rRNA 序列进行的系统发生学分析证明，这两株菌株是弧菌属的成员，属于 Ponticus 支系。然后，利用从海洋生物和水生环境中分离的四株模式菌株和四株参考菌株进行了系统进化分析。139 个单拷贝基因的多焦点序列分析（MLSA）表明，CAIM 703 和 CAIM 1902 属于 V. panuliri。CAIM 703 和 CAIM 1902 的 16S rRNA 序列相似度为 99.61%。除了 V. panuliri LBS2T（相似度为 99% 和 100%）外，其他 Ponticus 支系物种与这两个菌株的 ANIb 平均核苷酸相同度（ANI）值在 78% 至 80% 之间。最后，该分析代表了对 Ponticus 支系的首次系统发生组分析，其中 V. panuliri 菌株来自墨西哥。

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Antonie van Leeuwenhoek

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