Dissociation Phenomenon of Erythrocyte Agglutination and Its Application to Assay of Functional Activity of the Complement System in Clinical Laboratory

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY Journal of Clinical Laboratory Analysis Pub Date : 2024-03-20 DOI:10.1002/jcla.25028

Xuewei Ding, Lina Liu, Guang Yang, Hui Liu

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Abstract

Objective

The objective of the study was to validate the dissociation phenomenon of erythrocyte agglutination which is based on erythrocyte fragments and to apply it in the functional activity assay of the complement system.

Methods

The dissociation–agglutination effect of erythrocyte fragments was validated by detecting the number of free erythrocytes after the action of erythrocyte fragments on agglutinated erythrocytes. The number of free erythrocytes produced after hemolysis of agglutinated erythrocytes caused by complements and complement activators(CAs) was detected by auto hematology analyzer and the results were indicated by mean hemoglobin concentration of erythrocytes (MCHC). We optimized the test conditions and validated the inter-batch stability, explored the resolution of the assay method, and assayed for the total complement activity (AC) and the CAs activated complement activity (ACA) in serum from patients and healthy individual groups.

Results

Erythrocyte fragments have a dissociative effect on agglutinated erythrocytes. The auto hematology analyzer was able to detect AC and ACA, where AC showed an inverse correlation with MCHC, and ACA demonstrated a positive correlation with MCHC. The inter-batch CV of AC, ACA, and ACA/AC was found to be 5%, 9%, and 11.7%, respectively, with good stability. The study found that serum samples from acute phase reaction patients showed significant differences in ACA compared with healthy individuals, with a p value of 0.018; serum samples from patients with nephrotic syndrome showed significant differences in AC, ACA, and ACA/AC compared with healthy individuals, with p values of 0.014, 0.002, and 0.041, respectively.

Conclusion

Erythrocyte fragments have dissociation–agglutination effect. The complement system immunological functional detection method, based on this effect, has potential clinical application value due to its sensitivity and accuracy.

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红细胞凝集的解离现象及其在临床实验室检测补体系统功能活性中的应用。

研究目的研究目的是验证基于红细胞片段的红细胞凝集解离现象，并将其应用于补体系统的功能活性测定：方法：通过检测红细胞碎片作用于凝集红细胞后游离红细胞的数量来验证红细胞碎片的解离-凝集效应。用自动血液分析仪检测补体和补体激活剂（CAs）对凝集红细胞溶血后产生的游离红细胞数量，结果用红细胞平均血红蛋白浓度（MCHC）表示。我们优化了检测条件，验证了批间稳定性，探索了检测方法的分辨率，并检测了患者和健康人血清中的总补体活性（AC）和CAs激活的补体活性（ACA）：结果：红细胞碎片对凝集的红细胞有解离作用。自动血液分析仪能检测出 AC 和 ACA，其中 AC 与 MCHC 呈反相关，而 ACA 与 MCHC 呈正相关。研究发现，AC、ACA 和 ACA/AC 的批间 CV 分别为 5%、9% 和 11.7%，稳定性良好。研究发现，急性期反应患者血清样本的 ACA 与健康人相比有显著差异，P 值为 0.018；肾病综合征患者血清样本的 AC、ACA 和 ACA/AC 与健康人相比有显著差异，P 值分别为 0.014、0.002 和 0.041：结论：红细胞碎片具有解离-凝集作用。结论：红细胞碎片具有解离-凝集效应，基于该效应的补体系统免疫功能检测方法因其灵敏性和准确性而具有潜在的临床应用价值。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.