Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-08-01 Epub Date: 2024-03-21 DOI:10.1007/s00449-024-02995-5
Gopinath Venkatraman, Priyadarshini Sakthi Mohan, Puteri Shafinaz Abdul-Rahman, Faridah Sonsudin, Barathan Muttiah, Abdurahman Hajinur Hirad, Abdullah A Alarfaj, Shifa Wang
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Abstract

This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 μg/mL for Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, Staphylococcus aureus than ZnO NPs at the MIC of 120 and 140 μg/mL for Bacillus subtilis and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Furthermore, ROS detection, viability assay and bacterial membrane integrity analysis of ZnO/Ag NCs treated P. aeruginosa and S. aureus revealed the fundamental bactericidal mechanism involving cell wall, cell membrane interaction and release of cytoplasmic contents. In addition, ZnO/Ag NCs and ZnO NPs showed higher toxicity towards A549 lung cancer cells than the non-cancerous RAW264 macrophage cells, with IC50 of 242 and 398 µg/mL respectively, compared to IC50 of 402 and 494 µg/mL for the macrophage cells. These results suggest that the ZnO/Ag NCs can be effectively used to develop antimicrobial and anticancer materials.

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海巴戟叶辅助合成氧化锌装饰银生物纳米复合材料,用于体外细胞毒性、抗菌和抗癌应用。
本研究利用海巴戟叶(MCL)提取物合成了氧化锌纳米颗粒(ZnO NPs)和氧化锌装饰银纳米复合材料(ZnO/Ag NCs)。利用场发射扫描电子显微镜(FESEM)和 X 射线衍射(XRD)分析对合成的纳米材料的结构形态和结晶度进行了表征。利用人体病原菌对氧化锌纳米粒子和氧化锌/银纳米粒子的抗菌活性进行了评估。ZnO/Ag NCs 对大肠杆菌、绿脓杆菌、枯草芽孢杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为 80 和 100 μg/mL,而 ZnO NPs 对枯草芽孢杆菌、大肠杆菌、绿脓杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为 120 和 140 μg/mL,因此 ZnO/Ag NCs 的抗菌活性最高。此外,氧化锌/银数控材料处理铜绿假单胞菌和金黄色葡萄球菌的 ROS 检测、活力测定和细菌膜完整性分析表明,其基本杀菌机制涉及细胞壁、细胞膜相互作用和细胞质内容物的释放。此外,ZnO/Ag NCs 和 ZnO NPs 对 A549 肺癌细胞的毒性高于非癌细胞 RAW264 巨噬细胞,其 IC50 分别为 242 和 398 µg/mL,而巨噬细胞的 IC50 分别为 402 和 494 µg/mL。这些结果表明,ZnO/Ag NCs 可有效用于开发抗菌和抗癌材料。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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