Effects of zinc oxide nanoparticles on carbon mineralization kinetics and microbial attributes in plant residue-amended soils

Abstract

This study was designed to determine the impacts of different ZnO-NPs concentrations (100–1000 mg kg⁻¹ soil) on soil carbon (C) mineralization of a calcareous soil amended with alfalfa hay (AH) and wheat straw (WS). In addition, C mineralization kinetics in the residue-amended soils were described using different kinetic models and the impacts of ZnO-NPs on the kinetic parameters were investigated. The microbial biomass C (MBC) and basal respiration (BR) were also compared in the treated and control soil samples. The findings revealed a significant reduction in total cumulative mineralized C (C_min) in soil amended with AH at ZnO-NPs concentrations of 100 and 200 mg⁻¹ kg soil. Similarly, in soil samples amended with WS, the C_min value demonstrated a significant decrease across all ZnO-NPs concentrations, except for the lowest concentration of 100 mg⁻¹ kg soil. The results of kinetic analysis using the double first-order model showed that the introduction of ZnO-NPs led to an increase of up to 147 % in the amount of C mineralization during the initial fast phase, while it also resulted in a reduction of up to 27.3 % in C mineralization during the subsequent slow phase. Moreover, the presence of ZnO-NPs in the soil resulted in a noteworthy decrease of 4.44 % to 54.5 % in MBC and 5.12 % to 35.7 % in BR values when compared to the uncontaminated soil, suggesting that the size and activity of the soil microbial community were suppressed, with the extent of suppression varying depending on the type of plant residues and concentrations of ZnO-NPs applied. These findings strongly suggest that the soil microbial community was subjected to heightened toxicity stress caused by ZnO-NPs, particularly at higher concentrations. In conclusion, ZnO-NPs can drastically influence the microbial abundance, maintenance energy demand, and C mineralization process in plant residue-amended calcareous soils.