Interactions between commensal Enterococcus faecium and Enterococcus lactis and clinical isolates of E. faecium

T. Wagner, A. K. Pöntinen, Carolin Kornelia Fenzel, Daniel Engi, J. Janice, Ana C. Almeida-Santos, A. Tedim, Ana R. Freitas, Luísa Peixe, W. van Schaik, Mona Johannessen, K. Hegstad
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Abstract

Enterococcus faecium (Efm) is a versatile pathogen, responsible for multidrug-resistant infections, especially in hospitalized immunocompromised patients. Its population structure has been characterized by diverse clades (A1, A2 and B (reclassified as Enterococcus lactis (Ela)), adapted to different environments, and distinguished by their resistomes and virulomes. These features only partially explain the predominance of clade A1 strains in nosocomial infections. We investigated in vitro interaction of 50 clinical isolates (clade A1 Efm) against 75 commensal faecal isolates from healthy humans (25 clade A2 Efm and 50 Ela). Only 36% of the commensal isolates inhibited clinical isolates, while 76% of the clinical isolates inhibited commensal isolates. The most apparent overall differences in inhibition patterns were presented between clades. The inhibitory activity was mainly mediated by secreted, proteinaceous, heat-stable compounds, likely indicating an involvement of bacteriocins. A custom-made database targeting 76 Bacillota bacteriocins was used to reveal bacteriocins in the genomes. Our systematic screening of the interactions between nosocomial and commensal Efm and Ela on a large scale suggests that, in a clinical setting, nosocomial strains not only have an advantage over commensal strains due to their possession of AMR genes, virulence factors, and resilience but also inhibit the growth of commensal strains.
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共生粪肠球菌和乳肠球菌与临床分离的粪肠球菌之间的相互作用
粪肠球菌(Enterococcus faecium,Efm)是一种多用途病原体,是耐多药感染的罪魁祸首,尤其是在住院的免疫力低下患者中。它的种群结构以不同的支系(A1、A2 和 B(重新分类为乳酸肠球菌(Ela))为特征,这些支系适应不同的环境,并以抗药性和毒力组来区分。这些特征只能部分解释 A1 支系菌株在医院感染中占主导地位的原因。我们研究了 50 株临床分离株(A1 族 Efm)与 75 株健康人粪便共生分离株(25 株 A2 族 Efm 和 50 株 Ela)的体外相互作用。只有 36% 的共生分离物对临床分离物有抑制作用,而 76% 的临床分离物对共生分离物有抑制作用。不同支系之间抑制模式的总体差异最为明显。抑制活性主要由分泌型、蛋白型、热稳定型化合物介导,这可能表明细菌素的参与。我们利用一个针对 76 种芽孢杆菌细菌素的定制数据库来揭示基因组中的细菌素。我们对鼻腔菌株与普通菌株 Efm 和 Ela 之间的相互作用进行了大规模的系统筛选,结果表明,在临床环境中,鼻腔菌株不仅因拥有 AMR 基因、毒力因子和恢复能力而比普通菌株更具优势,而且还能抑制普通菌株的生长。
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来源期刊
CiteScore
3.30
自引率
0.00%
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0
审稿时长
15 weeks
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