Comprehensive analysis of the immune implication of EPHX4 gene in laryngeal squamous cell carcinoma

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY Brazilian Journal of Otorhinolaryngology Pub Date : 2024-03-07 DOI:10.1016/j.bjorl.2024.101411

Nimei Shen , Gang Gao , Xinhong Lu, Jiaxin Jin, Liwei Lin, Maohua Qian, Yang Qin

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Abstract

Objectives

The role of Epoxide Hydrolase-4 (EPHX4), a member of epoxide hydrolase family, has not been investigated in cancer. The purpose of this article is to explore the application value of EPHX4 in laryngeal cancer and its relationship with immune infiltration.

Methods

We observed that EPHX4 expression and its survival assays in laryngeal cancer specimens based on The Cancer Genome Atlas (TCGA) cohorts. We also analyzed the correlation between immune cell infiltration levels and EPHX4 gene copy number in laryngeal cancer. Finally, we conducted in vitro assay to evaluate the functions of EPHX4 in laryngeal cancer cell line.

Results

EPHX4 is highly expressed in laryngeal cancer specimens and has a poor prognosis. EPHX4 related immune cell analysis showed that it participated in NK Natural killer cell mediated cytotoxicity. Finally, Cell experiments indicate that EPHX4 could promote laryngeal cancer cell line proliferation, colony formation and invasion.

Conclusions

Our research results suggest that EPHX4 may be a potential immunotherapy target for laryngeal cancer. The nominated immune signature is a helpful and promising prognostic indicator in laryngeal cancer.

Levels of Evidence

Level 3.

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全面分析EPHX4基因在喉鳞癌中的免疫影响

目的环氧化物水解酶家族成员之一环氧化物水解酶-4（EPHX4）在癌症中的作用尚未得到研究。本文旨在探讨 EPHX4 在喉癌中的应用价值及其与免疫浸润的关系。方法我们观察了基于癌症基因组图谱（TCGA）队列的喉癌标本中 EPHX4 的表达及其生存测定。我们还分析了喉癌中免疫细胞浸润水平与 EPHX4 基因拷贝数之间的相关性。结果EPHX4在喉癌标本中高表达，且预后较差。与 EPHX4 相关的免疫细胞分析表明，它参与了 NK 自然杀伤细胞介导的细胞毒性。最后，细胞实验表明，EPHX4 可促进喉癌细胞株的增殖、集落形成和侵袭。我们的研究结果表明，EPHX4 可能是喉癌的潜在免疫治疗靶点。提名的免疫特征是喉癌的一个有用且有前景的预后指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brazilian Journal of Otorhinolaryngology OTORHINOLARYNGOLOGY-

CiteScore

3.00

自引率

0.00%

发文量

205

审稿时长

4-8 weeks

期刊介绍： Brazilian Journal of Otorhinolaryngology publishes original contributions in otolaryngology and the associated areas (cranio-maxillo-facial surgery and phoniatrics). The aim of this journal is the national and international divulgation of the scientific production interesting to the otolaryngology, as well as the discussion, in editorials, of subjects of scientific, academic and professional relevance. The Brazilian Journal of Otorhinolaryngology is born from the Revista Brasileira de Otorrinolaringologia, of which it is the English version, created and indexed by MEDLINE in 2005. It is the official scientific publication of the Brazilian Association of Otolaryngology and Cervicofacial Surgery. Its abbreviated title is Braz J Otorhinolaryngol., which should be used in bibliographies, footnotes and bibliographical references and strips.