Rational construction of synthetic consortia: Key considerations and model-based methods for guiding the development of a novel biosynthesis platform

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-03-24 DOI:10.1016/j.biotechadv.2024.108348
Yu Liu , Boyuan Xue , Hao Liu, Shaojie Wang, Haijia Su
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Abstract

The rapid development of synthetic biology has significantly improved the capabilities of mono-culture systems in converting different substrates into various value-added bio-chemicals through metabolic engineering. However, overexpression of biosynthetic pathways in recombinant strains can impose a heavy metabolic burden on the host, resulting in imbalanced energy distribution and negatively affecting both cell growth and biosynthesis capacity. Synthetic consortia, consisting of two or more microbial species or strains with complementary functions, have emerged as a promising and efficient platform to alleviate the metabolic burden and increase product yield. However, research on synthetic consortia is still in its infancy, with numerous challenges regarding the design and construction of stable synthetic consortia. This review provides a comprehensive comparison of the advantages and disadvantages of mono-culture systems and synthetic consortia. Key considerations for engineering synthetic consortia based on recent advances are summarized, and simulation and computational tools for guiding the advancement of synthetic consortia are discussed. Moreover, further development of more efficient and cost-effective synthetic consortia with emerging technologies such as artificial intelligence and machine learning is highlighted.

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合成联合体的合理构建:指导新型生物合成平台开发的主要考虑因素和基于模型的方法。
合成生物学的快速发展大大提高了单培养系统通过代谢工程将不同底物转化为各种高附加值生物化学品的能力。然而,在重组菌株中过度表达生物合成途径会给宿主带来沉重的代谢负担,导致能量分配失衡,对细胞生长和生物合成能力产生负面影响。由两种或两种以上具有互补功能的微生物物种或菌株组成的合成联合体,已成为减轻代谢负担和提高产品产量的一种前景广阔的高效平台。然而,合成联合体的研究仍处于起步阶段,在设计和构建稳定的合成联合体方面存在诸多挑战。本综述全面比较了单培养系统和合成联合体的优缺点。总结了基于最新进展的合成联合体工程的主要考虑因素,并讨论了指导合成联合体发展的模拟和计算工具。此外,还强调了利用人工智能和机器学习等新兴技术进一步开发更高效、更具成本效益的合成联合体。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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