Pub Date : 2026-02-07DOI: 10.1016/j.biotechadv.2026.108835
Benedict Ryan Lukito, Naazneen Sofeo, Hui Jean Lim, Muhammad Harith bin Mohammad Taufik, Prakash Arumugam, Aiqun Yu, Adison Wong
{"title":"Expanding biotechnological applications of Yarrowia lipolytica: Key advances in the past decade","authors":"Benedict Ryan Lukito, Naazneen Sofeo, Hui Jean Lim, Muhammad Harith bin Mohammad Taufik, Prakash Arumugam, Aiqun Yu, Adison Wong","doi":"10.1016/j.biotechadv.2026.108835","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2026.108835","url":null,"abstract":"","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"110 1","pages":""},"PeriodicalIF":16.0,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146134405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-07DOI: 10.1016/j.biotechadv.2026.108840
Mengyang Zhang, Jia Wu, Jie Zhang, Fu Wang, Youcheng Wang, Xiujuan Lei, Yingping Wang, Jian Zhang
{"title":"Biotechnological advances in key regulatory genes of phenylpropanoid and terpenoid biosynthesis pathways in Panax ginseng: Current insights and future prospects","authors":"Mengyang Zhang, Jia Wu, Jie Zhang, Fu Wang, Youcheng Wang, Xiujuan Lei, Yingping Wang, Jian Zhang","doi":"10.1016/j.biotechadv.2026.108840","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2026.108840","url":null,"abstract":"","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"46 1","pages":""},"PeriodicalIF":16.0,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146134406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-04DOI: 10.1016/j.biotechadv.2026.108837
Xiaoxiao Jiang, Yujie Wang, Zhanyu Wang, Xu Yang, Yuguang Mu, Rui Zhai, Tao Wei, Mingjie Jin
The recalcitrance of lignocellulosic biomass, stemming from its complex cellulose-hemicellulose-lignin matrix, remains the primary techno-economic bottleneck in sugar-platform biorefineries. Surfactants have emerged as versatile process-intensifying agents capable of overcoming these interfacial and chemical barriers. While previous reviews have largely focused on macroscopic yield improvements, a critical synthesis elucidating the molecular-level surfactant-biomass-enzyme interplay is lacking. This review provides a comprehensive analysis of surfactant-mediated mechanisms across both pretreatment and enzymatic hydrolysis. Uniquely, we highlight the role of surfactants beyond physical dominance, detailing their capacity to induce in-situ chemical modifications of lignin during pretreatment. Mechanisms such as surfactant grafting via α-etherification, phenolic hydroxyl blocking, and C5 position stabilization are critically examined for their roles in preventing lignin condensation and mitigating downstream enzyme inhibition. Furthermore, we elucidate how surfactants modulate interfacial phenomena during hydrolysis, from shielding non-productive lignin adsorption sites to stabilizing enzyme conformation against shear and thermal stresses. Finally, the review outlines a roadmap for transitioning from empirical screening to the rational design of sustainable, multi-functional surfactants, emphasizing their integration into closed-loop biorefinery processes.
{"title":"Surfactants as process intensifiers in lignocellulosic sugar-platform biorefineries: Mechanistic insights and bioprocess implications.","authors":"Xiaoxiao Jiang, Yujie Wang, Zhanyu Wang, Xu Yang, Yuguang Mu, Rui Zhai, Tao Wei, Mingjie Jin","doi":"10.1016/j.biotechadv.2026.108837","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2026.108837","url":null,"abstract":"<p><p>The recalcitrance of lignocellulosic biomass, stemming from its complex cellulose-hemicellulose-lignin matrix, remains the primary techno-economic bottleneck in sugar-platform biorefineries. Surfactants have emerged as versatile process-intensifying agents capable of overcoming these interfacial and chemical barriers. While previous reviews have largely focused on macroscopic yield improvements, a critical synthesis elucidating the molecular-level surfactant-biomass-enzyme interplay is lacking. This review provides a comprehensive analysis of surfactant-mediated mechanisms across both pretreatment and enzymatic hydrolysis. Uniquely, we highlight the role of surfactants beyond physical dominance, detailing their capacity to induce in-situ chemical modifications of lignin during pretreatment. Mechanisms such as surfactant grafting via α-etherification, phenolic hydroxyl blocking, and C5 position stabilization are critically examined for their roles in preventing lignin condensation and mitigating downstream enzyme inhibition. Furthermore, we elucidate how surfactants modulate interfacial phenomena during hydrolysis, from shielding non-productive lignin adsorption sites to stabilizing enzyme conformation against shear and thermal stresses. Finally, the review outlines a roadmap for transitioning from empirical screening to the rational design of sustainable, multi-functional surfactants, emphasizing their integration into closed-loop biorefinery processes.</p>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":" ","pages":"108837"},"PeriodicalIF":12.5,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-04DOI: 10.1016/j.biotechadv.2026.108830
Christina Pentari, Eleni Krassa, Anastasia Zerva, Evangelos Topakas
{"title":"Glycobiohydrolases at the forefront of lignocellulose saccharification: A review on substrate specificities and structure-function properties","authors":"Christina Pentari, Eleni Krassa, Anastasia Zerva, Evangelos Topakas","doi":"10.1016/j.biotechadv.2026.108830","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2026.108830","url":null,"abstract":"","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"5 1","pages":""},"PeriodicalIF":16.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146110545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Photo-fermentative hydrogen production from lignocellulosic biomass offers a sustainable and carbon-neutral route for bioenergy conversion, providing an effective strategy to mitigate fossil resource depletion and greenhouse gas emissions. Among diverse microbial candidates, purple non‑sulfur bacteria (PNSB) have emerged as promising photosynthetic platforms owing to their broad substrate utilization, intrinsic tolerance to lignocellulose-derived inhibitors, and high hydrogen yields under light-anaerobic conditions. Their metabolic versatility allows dynamic redistribution of carbon and electron fluxes, facilitating efficient energy conversion. Recent progress in metabolic engineering has substantially advanced the hydrogen-producing capacity of PNSB through targeted manipulation of photosynthetic metabolism, redox balance and stress response pathways. Engineering strategies have focused on optimizing pigment biosynthesis to enhance light harvesting, reinforcing redox homeostasis and adenosine triphosphate (ATP) generation, and improving tolerance to environmental stresses such as ammonia, pH, and temperature fluctuations. These efforts have led to engineered strains exhibiting extraordinary improvement in hydrogen yield, stability and robustness. This review provides an overview of the fundamental mechanisms underlying photo-fermentative hydrogen metabolism in PNSB, summarizes recent advances in the metabolic and systems-level engineering strategies, and outlines the prospects of developing strains capable of approaching the theoretical limit of hydrogen yield through integrated engineering strategies, advanced tools such as CRISPR-Cas, and adaptive laboratory evolution methods.
{"title":"Recent advances in metabolic engineering of purple non‑sulfur photosynthetic bacteria for enhanced biohydrogen production.","authors":"Wen Cao, Jiyan Lu, Youmin Jiang, Maosen Yuan, Minmin Wang, Xuefang Mu, Zixuan Gao, Siyi Yang, Tengteng Li, Xuan Wei, Liejin Guo","doi":"10.1016/j.biotechadv.2026.108832","DOIUrl":"10.1016/j.biotechadv.2026.108832","url":null,"abstract":"<p><p>Photo-fermentative hydrogen production from lignocellulosic biomass offers a sustainable and carbon-neutral route for bioenergy conversion, providing an effective strategy to mitigate fossil resource depletion and greenhouse gas emissions. Among diverse microbial candidates, purple non‑sulfur bacteria (PNSB) have emerged as promising photosynthetic platforms owing to their broad substrate utilization, intrinsic tolerance to lignocellulose-derived inhibitors, and high hydrogen yields under light-anaerobic conditions. Their metabolic versatility allows dynamic redistribution of carbon and electron fluxes, facilitating efficient energy conversion. Recent progress in metabolic engineering has substantially advanced the hydrogen-producing capacity of PNSB through targeted manipulation of photosynthetic metabolism, redox balance and stress response pathways. Engineering strategies have focused on optimizing pigment biosynthesis to enhance light harvesting, reinforcing redox homeostasis and adenosine triphosphate (ATP) generation, and improving tolerance to environmental stresses such as ammonia, pH, and temperature fluctuations. These efforts have led to engineered strains exhibiting extraordinary improvement in hydrogen yield, stability and robustness. This review provides an overview of the fundamental mechanisms underlying photo-fermentative hydrogen metabolism in PNSB, summarizes recent advances in the metabolic and systems-level engineering strategies, and outlines the prospects of developing strains capable of approaching the theoretical limit of hydrogen yield through integrated engineering strategies, advanced tools such as CRISPR-Cas, and adaptive laboratory evolution methods.</p>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":" ","pages":"108832"},"PeriodicalIF":12.5,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1016/j.biotechadv.2026.108814
Charandatta Muddana, Binbin Wang, Pei-Ti Sun, Yinjie J. Tang
{"title":"Comparative evaluation of large language models for biotechnology review writing","authors":"Charandatta Muddana, Binbin Wang, Pei-Ti Sun, Yinjie J. Tang","doi":"10.1016/j.biotechadv.2026.108814","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2026.108814","url":null,"abstract":"","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"66 1","pages":""},"PeriodicalIF":16.0,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146110777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"From deep archival to real-time applications: Challenges and opportunities in DNA data storage","authors":"Qian Liu, Qiu-Jun Liu, Shaohua Kang, Jiawei Li, Haotian Xia, Hao Qi","doi":"10.1016/j.biotechadv.2026.108833","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2026.108833","url":null,"abstract":"","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"294 1","pages":""},"PeriodicalIF":16.0,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146110775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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