Oligoribonucleotide-Containing Nanocomplexes Based on Aminopropylsilanol Nanoparticles as Effective Inhibitors of Influenza A Virus Replication

Q3 Agricultural and Biological Sciences Moscow University Biological Sciences Bulletin Pub Date : 2024-03-25 DOI:10.3103/s0096392524600431

M. N. Repkova, O. Yu. Mazurkov, E. I. Filippova, M. A. Procenko, N. A. Mazurkova, B. P. Chelobanov, A. S. Levina, V. F. Zarytova

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Abstract

Titanium dioxide or aminopropylsilanol nanoparticles were shown to be effective vehicles for delivering oligodeoxyribonucleotides and deoxyribozymes to cells to affect target nucleic acids. In this paper, the proposed principle of the delivery has been implemented in relation to oligoribonucleotides (ORN), components of short interfering RNAs (siRNAs). It has been shown that the obtained ORN-containing nanocomplexes (Si~NH₂·ORN) based on aminopropylsilanol nanoparticles penetrate eukaryotic cells. These nanocomplexes have been investigated as agents for suppressing the replication of influenza A virus (IAV) in the cellular system. It has been shown that the ORN strands targeted to (+)RNA and (–)RNA of the IAV 5th segment reduces the titer of the virus by 99.7% and 98.4%, respectively. Thus, oligoribonucleotides in the Si~NH₂·ORN nanocomplexes effectively inhibit the replication of the influenza A virus.

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基于氨基丙基硅烷醇纳米颗粒的含寡核苷酸纳米复合物是甲型流感病毒复制的有效抑制剂

摘要二氧化钛或氨丙基硅醇纳米粒子已被证明是向细胞输送寡脱氧核苷酸和脱氧核酶以影响靶核酸的有效载体。本文提出的递送原理已在短干扰 RNA（siRNA）的组成成分--寡核苷酸（ORN）中得到应用。研究表明，基于氨基丙基硅烷醇纳米粒子获得的含寡核苷酸的纳米复合物（Si~NH2-ORN）可穿透真核细胞。这些纳米复合物已被研究用于抑制甲型流感病毒（IAV）在细胞系统中的复制。研究表明，针对 IAV 第 5 节段 (+)RNA 和 (-)RNA 的 ORN 链可将病毒滴度分别降低 99.7% 和 98.4%。因此，Si~NH2-ORN 纳米复合物中的寡核苷酸能有效抑制甲型流感病毒的复制。

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Moscow University Biological Sciences Bulletin Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

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期刊介绍： Moscow University Biological Sciences Bulletin is forum for research in all important areas of modern biology. It publishes original work on qualitative, analytical and experimental aspects of research. The scope of articles to be considered includes plant biology, zoology, ecology, evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, gerontology, developmental biology, bioinformatics, bioengineering, virology, and microbiology.