Exploring, Cloning, and Characterization of Aryl-alcohol Dehydrogenase for β-Phenylethanol Production in Paocai

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-03-27 DOI:10.1134/S0003683823602329

Z. Zhou, Y. Zhao, D. Peng, Z. Wu, W. Zhang

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Abstract

β-Phenylethanol is the primary flavor compound in Sichuan industrial paocai. Metatranscriptomics analysis revealed that the gene encoding aryl-alcohol dehydrogenase (AADh1), which is responsible for β-phenylethanol synthesis, had the highest expression level during the late stage of fermentation (180 days). In the present study, we explored the potential AADh1 in Sichuan industrial qingcai paocai by combining metatranscriptomics results and AADh1 analysis in NCBI. AADh1 was successfully cloned and expressed in Escherichia coli BL21, and the enzymatic properties of AADh1 were characterized. AADh1 exhibited the highest activity at pH 7.0 and 50°C, with the highest stability at pH 8.0 and 25°C. Furthermore, the enzyme exhibited low tolerance to metal ions, and only 1 mM Mn²⁺ could slightly activate the enzyme. Moreover, AADh1 was successfully used to preliminarily enhance the β-phenylethanol of industrial paocai, which could improve the flavor of paocai to a certain extent in practical production.

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探索、克隆和鉴定用于生产β-苯乙醇的芳基乙醇脱氢酶

摘要 β-苯乙醇是四川工业杷菜的主要风味化合物。元转录组学分析表明，负责合成β-苯乙醇的芳基醇脱氢酶（AADh1）基因在发酵后期（180 天）的表达水平最高。在本研究中，我们结合元转录组学结果和 NCBI 中的 AADh1 分析，探讨了四川工业化青菜中潜在的 AADh1。AADh1 被成功克隆并在大肠杆菌 BL21 中表达，AADh1 的酶特性也得到了表征。AADh1 在 pH 值为 7.0 和 50°C 时活性最高，在 pH 值为 8.0 和 25°C 时稳定性最高。此外，该酶对金属离子的耐受性较低，只有 1 mM Mn2+ 才能轻微激活该酶。此外，AADh1 被成功用于初步提高工业杷菜的β-苯乙醇含量，在实际生产中可在一定程度上改善杷菜的风味。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.