Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-03-28 DOI:10.1515/ract-2023-0260

Samia Hadj Rabia, Aicha Debib, Atika Eddaikra, Lila Aberkane-Mchebbek, Ramy Nouri, Fatima Benmoussa, Amine Mokhtari, Sarah Medjber, Belkacem Mansouri, Mohammed Messaoudi

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Abstract

The main objective of this research is to assess how gamma radiation influences the chemical composition and activities such as antioxidant, antimicrobial and anticancer activities of Lepidium sativum (L. sativum) seeds. Methanolic extracts were obtained through a 24 h maceration process from L. sativum seeds exposed to various doses of gamma irradiation. Phytochemical screening involved precipitation and staining reactions, with the antioxidant potential assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antimicrobial activity was evaluated through a diffusion test on agar medium, while the cytotoxic potential on the lung cancer line “A-549 cells” was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Qualitative analysis of the extracts identified preserved bioactive constituents after irradiation. Additionally, quantitative analysis indicated a significant rise in the total content of polyphenols and flavonoids under radiation, accompanied by an enhanced reducing power compared to the control. The antimicrobial and cytotoxic capabilities also improved, evident in increased growth inhibition zones for certain microbial strains and reduced viability rates of A549 cells. These results show promise and warrant further investigation into the potential of gamma radiation to enhance additional biological effects of plants.

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伽马辐照对鳞茎种子提取物的植物化学成分和生物活性的影响

这项研究的主要目的是评估伽马辐射如何影响莴苣（L. sativum）种子的化学成分和活性，如抗氧化、抗菌和抗癌活性。经不同剂量伽马辐照的莴苣种子通过 24 小时浸泡过程获得甲醇提取物。植物化学筛选包括沉淀和染色反应，并使用 2,2-二苯基-1-苦基肼（DPPH）测定法评估抗氧化潜力。抗菌活性通过琼脂培养基扩散试验进行评估，而对肺癌细胞株 "A-549 细胞 "的细胞毒性潜力则采用 3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-溴化四氮唑（MTT）测定法进行检测。对提取物进行的定性分析确定了辐照后保留的生物活性成分。此外，定量分析表明，与对照组相比，辐照后多酚和类黄酮的总含量显著增加，还原力也有所增强。抗菌和细胞毒性能力也有所提高，表现在某些微生物菌株的生长抑制区增大，A549 细胞的存活率降低。这些结果表明，伽马辐射有望增强植物的其他生物效应，值得进一步研究。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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