Inteins: A Swiss army knife for synthetic biology

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-03-27 DOI:10.1016/j.biotechadv.2024.108349
Stanislav Anastassov, Maurice Filo, Mustafa Khammash
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引用次数: 0

Abstract

Inteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using inteins, present approaches for overcoming these challenges, and detail their reliable use for specific cellular tasks. We will discuss the use of conditional inteins in areas like cancer therapy, drug screening, patterning, infection treatment, diagnostics and biocontainment. Additionally, we will underscore the potential of inteins in executing basic logical operations with practical implications. We conclude by showcasing their potential in crafting complex genetic circuits for performing computations and feedback control that achieves robust perfect adaptation.

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Inteins:合成生物学的瑞士军刀
内含蛋白是自然界中发现的执行蛋白质拼接的蛋白质。其中,分裂inteins以其多功能性和适应性脱颖而出,为应对各种生物应用中的复杂挑战提供了创造性的解决方案。它们精巧的特性,包括紧凑性、可靠性、正交性、低毒性和不可逆性,使它们在合成生物学、生物技术和生物医学等各个领域备受关注。在这篇综述中,我们将深入探讨使用inteins所面临的固有挑战,介绍克服这些挑战的方法,并详细介绍它们在特定细胞任务中的可靠应用。我们将讨论条件inteins在癌症治疗、药物筛选、模式化、感染治疗、诊断和生物封闭等领域的应用。此外,我们还将强调inteins在执行具有实际意义的基本逻辑运算方面的潜力。最后,我们将展示它们在制作复杂遗传电路以执行计算和反馈控制方面的潜力,从而实现稳健的完美适应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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