Characterizing arginine, ornithine, and putrescine pathways in enteric pathobionts

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2024-04-01 DOI:10.1002/mbo3.1408
Ian M. Lillie, Charles E. Booth, Adelaide E. Horvath, Matthew Mondragon, Melinda A. Engevik, Thomas D. Horvath
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Abstract

Arginine-ornithine metabolism plays a crucial role in bacterial homeostasis, as evidenced by numerous studies. However, the utilization of arginine and the downstream products of its metabolism remain undefined in various gut bacteria. To bridge this knowledge gap, we employed genomic screening to pinpoint relevant metabolic targets. We also devised a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics method to measure the levels of arginine, its upstream precursors, and downstream products in cell-free conditioned media from enteric pathobionts, including Escherichia coli, Klebsiella aerogenes, K. pneumoniae, Pseudomonas fluorescens, Acinetobacter baumannii, Streptococcus agalactiae, Staphylococcus epidermidis, S. aureus, and Enterococcus faecalis. Our findings revealed that all selected bacterial strains consumed glutamine, glutamate, and arginine, and produced citrulline, ornithine, and GABA in our chemically defined medium. Additionally, E. coli, K. pneumoniae, K. aerogenes, and P. fluorescens were found to convert arginine to agmatine and produce putrescine. Interestingly, arginine supplementation promoted biofilm formation in K. pneumoniae, while ornithine supplementation enhanced biofilm formation in S. epidermidis. These findings offer a comprehensive insight into arginine-ornithine metabolism in enteric pathobionts.

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鉴定肠道致病菌中的精氨酸、鸟氨酸和腐胺途径
大量研究证明,精氨酸-鸟氨酸代谢在细菌平衡中发挥着至关重要的作用。然而,精氨酸的利用及其代谢的下游产物在各种肠道细菌中仍未确定。为了弥补这一知识空白,我们采用了基因组筛选技术来确定相关的代谢靶标。我们还设计了一种有针对性的液相色谱-串联质谱(LC-MS/MS)代谢组学方法,以测量肠道致病菌(包括大肠埃希菌、产气克雷伯氏菌、肺炎克雷伯氏菌、流感假单胞菌等)无细胞条件培养基中精氨酸、其上游前体和下游产物的水平。肺炎双球菌、荧光假单胞菌、鲍曼不动杆菌、无乳链球菌、表皮葡萄球菌、金黄色葡萄球菌和粪肠球菌。我们的研究结果表明,在化学定义的培养基中,所有选定的细菌菌株都消耗谷氨酰胺、谷氨酸和精氨酸,并产生瓜氨酸、鸟氨酸和 GABA。此外,还发现大肠杆菌、肺炎双球菌、产气荚膜杆菌和荧光团菌能将精氨酸转化为矢车菊碱并产生腐胺。有趣的是,补充精氨酸可促进肺炎双球菌生物膜的形成,而补充鸟氨酸可促进表皮葡萄球菌生物膜的形成。这些发现为精氨酸-鸟氨酸在肠道致病菌中的代谢提供了一个全面的视角。
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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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