Catechol-O-methyltransferase and monoamine oxidase B inhibitory activities of Australian bee pollen.

IF 1.3 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-05-22 DOI:10.1093/bbb/zbae041

Chie Watanabe, Aoi Yanagihara, Ryo Miyata, Taichi Mitsui, Chihiro Honda, Daisuke Fujinami, Shigenori Kumazawa

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Abstract

Bee pollen is an apicultural product collected by honeybees from flower stamens and used as a functional food worldwide. In the present study, we aim to elucidate the functions of Australian bee pollen. Australian bee pollen extracts and their main components were tested for catechol-O-methyltransferase (COMT) and monoamine oxidase B (MAOB) inhibitory activities. These enzymes are key neurotransmitters involved in Parkinson's disease and depression. Myricetin (5), tricetin (6), and luteolin (7) exhibited high COMT inhibitory activities (half maximal inhibitory concentration [IC50] = 23.3, 13.8, and 47.4 µM, respectively). In contrast, 5, 7, and annulatin (8) exhibited MAOB inhibitory activities (IC50 = 89.7, 32.8, and 153 µM, respectively). Quantitative analysis via high-performance liquid chromatography revealed that 5 was abundant in Australian bee pollen extracts. Our findings suggest that 5 contributes to the COMT and MAOB inhibitory activities of Australian bee pollen.

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澳大利亚蜂花粉的儿茶酚-O-甲基转移酶和单胺氧化酶 B 抑制活性。

蜂花粉是蜜蜂从花蕊中采集的一种养蜂产品，在世界各地被用作功能性食品。本研究旨在阐明澳大利亚蜂花粉的功能。我们测试了澳大利亚蜂花粉提取物及其主要成分对儿茶酚-O-甲基转移酶（COMT）和单胺氧化酶 B（MAOB）的抑制活性。这些酶是与帕金森病和抑郁症有关的关键神经递质。杨梅素（5）、三叶素（6）和叶黄素（7）表现出较高的 COMT 抑制活性（半最大抑制浓度 [IC50] 分别为 23.3、13.8 和 47.4 µM）。相比之下，5、7 和胭脂虫素（8）具有 MAOB 抑制活性（IC50 分别为 89.7、32.8 和 153 µM）。通过高效液相色谱法进行的定量分析显示，5 在澳大利亚蜂花粉提取物中含量丰富。我们的研究结果表明，5有助于澳大利亚蜂花粉的 COMT 和 MAOB 抑制活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).