Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-12-01 Epub Date: 2024-04-01 DOI:10.1080/19336918.2024.2335881

Lei Zhang, Wenbang Chen, Xiaojun Li, Gengming Wang, Fubao Xing, Xiao Zhu

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Abstract

We aimed to investigate galectin-1 overexpression induces normal fibroblasts (NFs) translates into cancer-associated fibroblasts (CAFs). Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell. The motilities of H1299 and A549 cells were measured. Human umbilical vein endothelial cell (HUVEC) proliferation and tube formation ability were assessed. Tumor volume and tumor weight was recorded. Cells motilities were increased, while apoptosis rates were decreased after CMs co-cultured. B-cell lymphoma-2 (Bcl-2) expression level was increased, while Bcl2-associatedX (Bax) and cleaved-caspase3 decreased. CMs treatment enhanced HUVEC proliferation and tube formation. Tumor volume and weight in CMs treated mice were increased, and the sensitivity of anlotinib in co-cultured cells was decreased. Our results revealed that galectin-1 overexpression induced NFs translated into CAFs.

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Galectin-1过表达可诱导正常成纤维细胞转化为癌症相关成纤维细胞，并降低肺癌患者对安罗替尼的敏感性。

我们的目的是研究 galectin-1 过表达会诱导正常成纤维细胞（NFs）转化为癌症相关成纤维细胞（CAFs）。在人胚胎肺成纤维细胞（HFL1）中过表达 Galectin-1。测量了 H1299 和 A549 细胞的运动能力。评估人脐静脉内皮细胞（HUVEC）的增殖和管形成能力。记录肿瘤体积和重量。共培养 CMs 后，细胞运动能力增强，而细胞凋亡率降低。B细胞淋巴瘤-2（Bcl-2）表达水平升高，而Bcl2相关X（Bax）和裂解-caspase3表达水平降低。CMs处理可促进HUVEC增殖和管形成。经 CMs 处理的小鼠肿瘤体积和重量增加，共培养细胞对安罗替尼的敏感性降低。我们的研究结果表明，galectin-1过表达会诱导NFs转化为CAFs。

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