Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization.

IF 2 4区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-04-01 DOI:10.1080/10826068.2024.2332881
T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu
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Abstract

Synthetic dyes such as azo dyes are significant pollutants in the wastewater released from various textile industries. The low biodegradability and production from synthetic sources with high shelf life make azo dyes a challenging material for degradation. This study used chemically mutated Aspergillus terrus in the laccase production under solid-state fermentation using sugarcane bagasse. Initially, the wild-type strain produced a laccase activity of 4.12 U/mL. Later, the alkaline pretreatment of sugarcane bagasse showed a significant increase in laccase activity by 38.9%. Further, random mutagenesis treatment with 100 mM EMS generated a hyper laccase-producing strain with a 2.3-fold increment in laccase activity compared to the wild-type strain. The enzyme displayed optimal activity at pH 6.5 and 35 °C. The metal ions such as Fe3+ (29.4 U/mL), Fe2+ (20.8 U/mL) and Cu2+ (18.05 U/mL) showed positive effects on laccase activity. The crude laccase was used to bioremediate Congo red, a prominent azo dye used in textile and pharmaceutical industries. The preliminary studies with a crude enzyme displayed 68.86% dye decolourization after 24 h of incubation. Additionally, with Taguchi orthogonal array optimization experiments, the maximal dye decolorization of 78.24% was achieved by maintaining crude enzyme concentration (20 U), dye concentration (25 mg/L) and pH 4.5.

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利用甘蔗渣的价值,通过土曲霉高产生产漆酶,有效去除偶氮染料。
偶氮染料等合成染料是各种纺织工业排放的废水中的重要污染物。偶氮染料的生物可降解性低,且由合成来源生产,保质期长,因此是一种具有挑战性的降解材料。本研究利用化学变异的曲霉(Aspergillus terrus)在甘蔗渣固态发酵条件下生产漆酶。最初,野生型菌株产生的漆酶活性为 4.12 U/mL。随后,对甘蔗渣进行碱性预处理后,漆酶活性显著提高了 38.9%。此外,用 100 mM EMS 进行随机诱变处理产生了一种高产漆酶菌株,其漆酶活性比野生型菌株提高了 2.3 倍。该酶在 pH 值为 6.5 和温度为 35 ℃ 时显示出最佳活性。Fe3+(29.4 U/mL)、Fe2+(20.8 U/mL)和Cu2+(18.05 U/mL)等金属离子对漆酶活性有积极影响。粗制漆酶被用于对刚果红(一种用于纺织和制药行业的主要偶氮染料)进行生物处理。对粗酶的初步研究表明,培养 24 小时后,染料脱色率为 68.86%。此外,通过田口正交阵列优化实验,在保持粗酶浓度(20 U)、染料浓度(25 mg/L)和 pH 值为 4.5 的情况下,染料脱色率达到了 78.24%。
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来源期刊
Preparative Biochemistry & Biotechnology
Preparative Biochemistry & Biotechnology 工程技术-生化研究方法
CiteScore
4.90
自引率
3.40%
发文量
98
审稿时长
2 months
期刊介绍: Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.
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