Comparative analysis of human tear fluid and aqueous humor proteomes

IF 5.9 1区医学 Q1 OPHTHALMOLOGY Ocular Surface Pub Date : 2024-03-30 DOI:10.1016/j.jtos.2024.03.011

August Beisel , Garrett Jones , Joshua Glass , Tae Jin Lee , Marc Töteberg-Harms , Amy Estes , Lane Ulrich , Kathryn Bollinger , Shruti Sharma , Ashok Sharma

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Abstract

Purpose

Technological advancements allowing for the analysis of low-volume samples have led to the investigation of human tear fluid and aqueous humor (AH) as potential biomarker sources. However, acquiring AH samples poses significant challenges, making human tear fluid a more accessible alternative. This study aims to compare the protein compositions of these two biofluids to evaluate their suitability for biomarker discovery.

Methods

Paired tear and AH samples were collected from 20 patients undergoing cataract surgery. Tear samples were collected using Schirmer strips prior to surgery, and AH samples were collected from the anterior chamber immediately after corneal incision. Proteins were extracted and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results

A total of 481 proteins were identified in greater than 50% of the tear samples, and 191 proteins were detected in greater than 50% of the AH samples. Of these proteins, 82 were found to be common between the two biofluids, with ALB, LTF, TF, LCN1, and IGKC being the most abundant.

Conclusion

Although tear fluid and the AH are functionally independent and physically separated, many of the proteins detected in AH were also detected in tears. This direct comparison of the proteomic content of tear fluid and AH may aid in further investigation of tear fluid as a source of readily accessible biomarkers for various human diseases.

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人类泪液和眼房水蛋白质组的比较分析。

目的：分析低容量样本的技术进步促使人们将人类泪液和房水（AH）作为潜在的生物标记源进行研究。然而，获取 AH 样品面临着巨大的挑战，因此人类泪液成为更容易获取的替代品。本研究旨在比较这两种生物液体的蛋白质组成，以评估它们是否适合用于生物标记物的发现：方法：从 20 名接受白内障手术的患者身上采集了成对的泪液和 AH 样品。泪液样本是在手术前用施尔默试纸采集的，AH 样本是在角膜切开后立即从前房采集的。提取蛋白质并使用液相色谱-串联质谱法（LC-MS/MS）进行分析：结果：在超过 50% 的泪液样本中发现了 481 种蛋白质，在超过 50% 的 AH 样本中检测到了 191 种蛋白质。在这些蛋白质中，有 82 种蛋白质在两种生物流体中是共通的，其中含量最高的是 ALB、LTF、TF、LCN1 和 IGKC：结论：虽然泪液和AH在功能上是独立的，在物理上也是分离的，但在AH中检测到的许多蛋白质在泪液中也能检测到。直接比较泪液和AH的蛋白质组含量有助于进一步研究泪液作为各种人类疾病的易得生物标志物的来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ocular Surface 医学-眼科学

CiteScore

11.60

自引率

14.10%

发文量

审稿时长

39 days

期刊介绍： The Ocular Surface, a quarterly, a peer-reviewed journal, is an authoritative resource that integrates and interprets major findings in diverse fields related to the ocular surface, including ophthalmology, optometry, genetics, molecular biology, pharmacology, immunology, infectious disease, and epidemiology. Its critical review articles cover the most current knowledge on medical and surgical management of ocular surface pathology, new understandings of ocular surface physiology, the meaning of recent discoveries on how the ocular surface responds to injury and disease, and updates on drug and device development. The journal also publishes select original research reports and articles describing cutting-edge techniques and technology in the field. Benefits to authors We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services. Please see our Guide for Authors for information on article submission. If you require any further information or help, please visit our Support Center