Extrasynaptic distribution of NMDA receptors in cochlear inner hair cell afferent signaling complex

IF 2.7 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of chemical neuroanatomy Pub Date : 2024-04-01 DOI:10.1016/j.jchemneu.2024.102417

Juan Hong , Peidong Dai , Huazheng Liang , Guangbin Sun , Weidong Qi , Yong Bi

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Abstract

Objective

The distribution and role of NMDA receptors is unclear in the afferent signaling complex of the cochlea. The present study aimed to examine the distribution of NMDA receptors in cochlear afferent signaling complex of the adult mouse, and their relationship with ribbon synapses of inner hair cells (IHCs) and GABAergic efferent terminals of the lateral olivocochlear (LOC).

Methods

Immunofluorescence staining in combination with confocal microscopy was used to investigate the distribution of glutamatergic NMDA and AMPA receptors in afferent terminals of SGNs, and their relationship with ribbon synapses of IHCs and GABAergic efferent terminals of LOC.

Results

Terminals with AMPA receptors along with Ribbons of IHC formed afferent synapses in the basal pole of IHCs, and those with NMDA receptors were mainly distributed longitudinally in the IHCs nuclei region. Significant difference was found in the distribution of NMDA and AMPA receptors in IHC afferent signaling complex (P<0.05). Some GABAergic terminals colocalized with NMDA receptors at the IHC nucleus region (P>0.05).

Conclusion

There is significant difference in the distribution of NMDA and AMPA receptors in cochlear afferent signaling complex. NMDA receptors are present in the extra-synaptic region of ribbon synapses of IHCs, and they are related to GABA efferent terminals of the afferent signaling complex.

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耳蜗内毛细胞传入信号复合体中 NMDA 受体的突触外分布

目的 NMDA受体在耳蜗传入信号复合体中的分布和作用尚不清楚。本研究旨在探讨 NMDA 受体在成年小鼠耳蜗传入信号复合体中的分布及其与内毛细胞（IHC）带状突触和外侧橄榄耳（LOC）GABA 能传出终端的关系。方法采用免疫荧光染色结合共聚焦显微镜研究谷氨酸能NMDA和AMPA受体在SGN传入末端的分布，以及它们与IHC的带状突触和LOC的GABA能传出末端的关系。结果带有AMPA受体的终端与IHC的带状突触在IHC的基极形成传入突触，而带有NMDA受体的终端主要纵向分布在IHC的核区。NMDA和AMPA受体在IHC传入信号复合体中的分布存在显著差异（P<0.05）。结论 NMDA 和 AMPA 受体在耳蜗传入信号复合体中的分布存在显著差异。NMDA受体存在于IHC带状突触的突触外区域，它们与传入信号复合体的GABA传出终端有关。

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来源期刊

Journal of chemical neuroanatomy 医学-神经科学

CiteScore

4.50

自引率

3.60%

发文量

审稿时长

62 days

期刊介绍： The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.