Classical and molecular detection methods in aquatic environmental virology for waterborne diseases outbreaks

SIMÕES RSQ
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Abstract

Waterborne diseases commonly addresses the Human adenoviruses (HAdVs), Noroviruses (NoVs), Hepatitis A virus (HAV), Hepatitis E virus (HEV), Parvoviruses, Rotaviruses (RVs), Caliciviruses, and Enteroviruses including Coxsackieviruses and Polioviruses. Waterborne virus-based illness may be transmitted through drinking water contamination linked diseases transmission sources as seawater, freshwater and also sewage. This paper described the classical methods of detection in aquatic environmental virology as cell culture-based, immunoassay and molecular-based technologies. The implementation of molecular-based tools and wastewater-based epidemiology studies to high-throughput for aquatic biomonitoring should be up to date to mitigating the risk and impact on aquatic environments.
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水生环境病毒学中用于检测水传播疾病爆发的经典方法和分子检测方法
水传播疾病通常涉及人类腺病毒 (HAdVs)、诺如病毒 (NoVs)、甲型肝炎病毒 (HAV)、戊型肝炎病毒 (HEV)、副病毒 (Parvoviruses)、轮状病毒 (RVs)、卡利克病毒 (Caliciviruses) 和肠道病毒,包括柯萨奇病毒 (Coxsackieviruses) 和脊髓灰质炎病毒 (Polioviruses)。水媒病毒性疾病可通过与疾病传播源有关的饮用水污染传播,如海水、淡水和污水。本文介绍了基于细胞培养、免疫测定和分子技术的水生环境病毒学经典检测方法。基于分子的工具和基于废水的流行病学研究到高通量水生生物监测的实施应与时俱进,以减轻对水生环境的风险和影响。
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