{"title":"A comparative study on prevalence of uropathogens and their antibiogram in diabetics and non-diabetics attending a tertiary care hospital","authors":"Tanmay Anne, V. Suryadevara, Anuradha B.","doi":"10.18203/2320-6012.ijrms20240843","DOIUrl":null,"url":null,"abstract":"Background: Urinary tract infection (UTI) is a significant problem in both diabetics and non-diabetics. High glucose may create a culture medium for growth of the virulent organisms. Diabetics are at greater risk for developing complications of UTI. Extensive and improper use of antibiotics has caused widespread anti-microbial resistance among uro-pathogens. Indiscriminate use of antibiotics during Covid-19 pandemic might lead to more resistant uro-pathogens which might further complicate the treatment of UTI. This study will help to determine resistance patterns of common uro-pathogens, which is essential for proper patient care.\nMethods: Clean voided midstream urine samples were collected from 91 patients (67 diabetic and 24 non-diabetic). Urine cultures were performed using semi-quantitative technique and pathogens were identified using phenotypic methods. Those with colony forming units (CFU) ≥105 CFU/ml were subjected to antibiotic sensitivity testing by Kirby-Bauer disk diffusion method and the isolates were classified as sensitive, and resistant according to CLSI guidelines.\nResults: E. coli (53.84%) and Enterococci (29.67%) were the most commonly isolated pathogens of UTI in both diabetics and non-diabetics. E. coli resistance to imipenem was statistically more in diabetics when compared to non-diabetics (p=0.012). Resistance patterns of other organisms were similar in both the groups.\nConclusions: E. coli was the most common pathogen isolated in both groups followed by Enterococci and Klebsiella. Diabetics showed statistically significant higher resistance (100%) to imepenem than non-diabetics. Other organisms isolated in this study did not show any statistically significant difference in their antibiogram.","PeriodicalId":14210,"journal":{"name":"International Journal of Research in Medical Sciences","volume":"37 18","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Research in Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18203/2320-6012.ijrms20240843","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Urinary tract infection (UTI) is a significant problem in both diabetics and non-diabetics. High glucose may create a culture medium for growth of the virulent organisms. Diabetics are at greater risk for developing complications of UTI. Extensive and improper use of antibiotics has caused widespread anti-microbial resistance among uro-pathogens. Indiscriminate use of antibiotics during Covid-19 pandemic might lead to more resistant uro-pathogens which might further complicate the treatment of UTI. This study will help to determine resistance patterns of common uro-pathogens, which is essential for proper patient care.
Methods: Clean voided midstream urine samples were collected from 91 patients (67 diabetic and 24 non-diabetic). Urine cultures were performed using semi-quantitative technique and pathogens were identified using phenotypic methods. Those with colony forming units (CFU) ≥105 CFU/ml were subjected to antibiotic sensitivity testing by Kirby-Bauer disk diffusion method and the isolates were classified as sensitive, and resistant according to CLSI guidelines.
Results: E. coli (53.84%) and Enterococci (29.67%) were the most commonly isolated pathogens of UTI in both diabetics and non-diabetics. E. coli resistance to imipenem was statistically more in diabetics when compared to non-diabetics (p=0.012). Resistance patterns of other organisms were similar in both the groups.
Conclusions: E. coli was the most common pathogen isolated in both groups followed by Enterococci and Klebsiella. Diabetics showed statistically significant higher resistance (100%) to imepenem than non-diabetics. Other organisms isolated in this study did not show any statistically significant difference in their antibiogram.