IMPROVEMENT OF TECHNIQUES FOR TRANSPLANTING HEALTHY IN VITRO GRAPE PLANTS IN SOIL CULTURE CONDITIONS

Abstract

The article discusses techniques for improving the properties of a nutrient substrate used for planting healthy in vitro grape plants. The research was carried out on the basis of the laboratory of biotechnology ARRIV&W – branch of FSBSI FRARC on an interspecific hybrid Krasnostop Carpi. The purpuse of the study is to improve the ways of adapting healthy in vitro plants to non–sterile conditions. With microclonal reproduction of grapes, death most often occurs when transferring test tube plants to non-sterile conditions. The selection of the composition of the soil substrate and the improvement of its water-physical and biological properties helps to reduce the risk of plant death. In our studies, peat, garden soil and river sand (1:1:1) were used as part of the substrate, to which “Aquasin hydrogel” and “Trichoderma Veride” endomycorrhizal preparation containing mycelium and spores of Trichoderma viride fungi, strain 471, were added. The plants were planted in a 500 ml container, had been adapted to non-sterile conditions and had been grown for 90 days on racks of accelerated plant cultivation (RAPC). In the process, the indicators of the preservation of initial plants, their growth, development and spread of the root system were noted. As a result, it can be concluded that the combined use of hydrogel and mycorrhiza ensures the best survival and preservation of plants of the Krasnostop Carpi variety. The mycorrhizal preparation promotes better development of the leaf surface, and the addition of hydrogel ensures better development of the root system, which will provide plants with better survival in open ground conditions of the basic mother plantation. Key words: HEALTHY IN VITRO GRAPES, HYDROGEL, MYCORRHIZA, SUBSTRATE, ADAPTATION TO NON-STERILE CONDITIONS