Antioxidant Activity of Ethanol Extract on Qust Al Hindi (Saussurea lappa) Roots Using the FRAP Method

Dhevy Try Putry, Sukmawati Syarif, Rahmawati Rahmawati
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Abstract

Qust al hindi (Saussurea lappa) is a plant that has antioxidant activity, especially in the roots. Qust al hindi contains several chemical compounds including flavonoids, steroids, terpenes, alkaloids, sesquiterpenes, costunolide, dehydrococtus lactones, cynaropicrin, and chlorogenic acid. This study aims to determine the antioxidant activity of the ethanol extract of qust al hindi root using the FRAP method. Samples that had become powder were extracted using the soxhletation method with 96% ethanol and obtained a % rendition of 5.302%. The extract obtained was measured using a UV-Vis spectrophotometer at a maximum wavelength of 718 nm after being mixed with several FRAP reagents, using a quercetin standard. The results of the antioxidant analysis showed that the average antioxidant activity of the ethanol extract of the qust al hindi root (Saussurea lappa) using the Frap method was 10.3671 mgQE/g extract. Which means that the antioxidant activity of each g of extract is equivalent to the antioxidant activity of 10.3671 mg of quercetin.
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采用 FRAP 法研究 Qust Al Hindi(Saussurea lappa)根乙醇提取物的抗氧化活性
Qust al hindi(Saussurea lappa)是一种具有抗氧化活性的植物,尤其是在根部。昆布含有多种化合物,包括黄酮类、甾体类、萜烯类、生物碱、倍半萜类、costunolide、脱氢辛内酯、cynaropicrin 和绿原酸。本研究旨在利用 FRAP 法确定瞿麦根乙醇提取物的抗氧化活性。用 96% 的乙醇以索氏提取法提取粉末状样品,提取率为 5.302%。提取物与几种 FRAP 试剂混合后,用紫外可见分光光度计在 718 纳米的最大波长处进行测量,并使用槲皮素标准。抗氧化分析结果表明,采用 Frap 法,诃子根(Saussurea lappa)乙醇提取物的平均抗氧化活性为 10.3671 mgQE/g。这意味着每克提取物的抗氧化活性相当于 10.3671 毫克槲皮素的抗氧化活性。
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