The effect of low-level laser therapy on osteoclast differentiation: Clinical implications for tooth movement and bone density

IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Dental Sciences Pub Date : 2024-07-01 DOI:10.1016/j.jds.2024.03.023
Chun-Yi Huang , Huynh Hoai Thuong Le , Hsiao-Chi Tsai , Chih-Hsin Tang , Jian-Hong Yu
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Abstract

Background/purpose

Osteoclast differentiation is crucial for orchestrating both tooth movement and the maintenance of bone density. Therefore, the current study sought to explore the impact of low-level laser therapy (LLLT) on osteoclast differentiation, functional gene expression, molecular signaling pathways, and orthodontic tooth movement in clinical settings.

Materials and methods

The RAW 264.7 cell line served as the precursor for osteoclasts, and these cells underwent irradiation using a 808-nm LLLT. Osteoclast differentiation was assessed through tartrate-resistant acid phosphatase (TRAP) staining. Functional gene expression levels were evaluated using real-time quantitative polymerase chain reaction (RT-qPCR) while signaling molecules were examined through Western blot analysis. In the clinical study, 12 participants were enrolled. Their tooth movement was monitored using a TRIOS desktop scanner. Bone density measurements were conducted using Mimics software, which processed cone-beam computed tomography (CBCT) images exported in Digital Imaging and Communications in Medicine (DICOM) format.

Results

We found that LLLT effectively promoted receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation and the expression of osteoclast functional genes, including matrix metallopeptidase 9 (MMP9), nuclear factor of activated T-cells cytoplasmic 1(NFATc1), tartrate-resistant acid phosphatase (TRAP) and cathepsin K (CTSK) in RAW264.7 cells. Clinically, the cumulative tooth movement over 90 days was significantly higher in the laser group than in the control group.

Conclusion

Our research demonstrates that LLLT not only significantly promotes osteoclast differentiation but is also a valuable adjunct in orthodontic therapy.

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低强度激光疗法对破骨细胞分化的影响:对牙齿移动和骨密度的临床影响
背景/目的破骨细胞的分化对于协调牙齿移动和维持骨密度至关重要。因此,本研究试图探讨低强度激光疗法(LLLT)对破骨细胞分化、功能基因表达、分子信号通路和临床正畸牙齿移动的影响。材料与方法 RAW 264.7 细胞系作为破骨细胞的前体,这些细胞接受了 808 纳米 LLLT 的照射。通过耐酒石酸磷酸酶(TRAP)染色评估破骨细胞的分化情况。功能基因表达水平通过实时定量聚合酶链反应(RT-qPCR)进行评估,信号分子则通过 Western 印迹分析进行检测。在临床研究中,共有 12 名参与者参加。他们使用 TRIOS 台式扫描仪监测牙齿移动情况。骨密度测量使用 Mimics 软件进行,该软件可处理以数字成像和医学通信 (DICOM) 格式导出的锥束计算机断层扫描 (CBCT) 图像。结果我们发现,LLLT能有效促进RAW264.7细胞中核因子κB配体受体激活剂(RANKL)依赖性破骨细胞的分化和破骨细胞功能基因的表达,包括基质金属肽酶9(MMP9)、活化T细胞胞浆核因子1(NFATc1)、抗酒石酸磷酸酶(TRAP)和凝血酶K(CTSK)。结论:我们的研究表明,LLLT 不仅能显著促进破骨细胞的分化,还是正畸治疗中的一种重要辅助手段。
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来源期刊
Journal of Dental Sciences
Journal of Dental Sciences 医学-牙科与口腔外科
CiteScore
5.10
自引率
14.30%
发文量
348
审稿时长
6 days
期刊介绍: he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases. The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.
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