In Vitro Anticancer Efficacy of Hydroalcoholic Extract From Crateva religiosa G. Forst. Bark on Human Ovarian Cancer Cells

Abstract

Introduction

Medical research on cancer treatment is getting worldwide attention in the present day. The usage of plant sources for cancer treatment is getting much consideration because of their low toxic nature, easy availability, and with fewer side effects. This study illustrates the anticancer potential of Crateva religiosa G. Forst. extract against human ovarian cancer cells (PA-1).

Methods

Cell viability was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and acridine orange/ethidium bromide staining techniques were used to evaluate the cell death and anticancer activity. Additionally, antioxidant activities such as hydroxyl-radical scavenging, 2,2-diphenylpicrylhydrazyl, ferric reducing ability of plasma, and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate⁺ were carried out. Moreover, phytochemical profiling and their quantification were also performed.

Results

The hydroalcoholic bark extract of C. religiosa has a strong anticancer prospective against PA-1 with a half-inhibitory concentration value of 33.27 μg/ml. The antioxidant potential of the extract showed potent activity on various antioxidants. The phytochemical profiling of the extract showed the presence of various phytochemicals and the quantification indicated the presence of a total phenol content of 2.1 ± 0.09 mg Gallic acid equivalent/g, total flavonoid content of 1.706 ± 0.01 mg CE/g, total alkaloid content of 1.237 ± 0.03 mg AE/g, and total terpenoid content of 1.004 ± 0.06 mg UAE/g.

Conclusions

The current findings of the study suggest the role of C. religiosa bark against human ovarian cancer cells (PA-1) and could be a potential drug source for the discovery of anticancer agents.