MOLECULAR CHARACTERIZATION OF ADENOVIRUS AND ROTAVIRUS ASSOCIATED DIARRHOEA AMONG UNDER-FIVES IN TWO TERTIARY HOSPITALS IN SOUTHERN NIGERIA

Dirisu John Osilumie, Agbakoba Nneka Regina, Okwelogu Somadina Izunna, Eki-Udoko Fidelis E., E. Jacinta C., O. Olukayode
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Abstract

Background: Viral diarrhoea continues to be a challenge among children under five years of age in Nigeria. While other non-viral agents of diarrhoea have been well documented, the agents of viral diarrhoea especially their genetic diversity have not been well studied in our locality, hence this study. Method: This was a cross-sectional study carried out at the University of Benin Teaching Hospital (UBTH) and NnamdiAzikiwe University Teaching Hospital (NAUTH) in Benin City and Nnewi respectively. Three hundred and thirty participants were recruited for the study using stratified random sampling, aged between 1 and 5 years. All the diarrhoeic stools had their DNA/RNA extracted using the Quick-RNA Viral MiniPrep Kit and stored at -80°C for polymerase chain reaction. Sequences of the adenovirus were done using a Big Dye DNA sequencing kit. Bioinformatics was done using R packages in R studio v3 software. Data from the study were analyzed using chi-square and level of significance expressed using p-value (p<0.05). Results: Adenoviral diarrhoea was higher in Benin City (18 11.8%) than Nnewi (11 6.1%). Females (55.2%) were more infected than the males (44.8%) (p>0.05). Among the population studied, there was no rotavirus genome detected. Ten (10) adenovirus sequences from serotype 40 were identified and deposited into the National Centre for Biotechnology Information (Genbank: http://www.ncbi.nlm.nih.gov/) under the accession number ON128719 - ON128728. The prevalence of adenovirus in the study locality is 8.7%. More females were infected than the males, although not statistically significant (p>0.05). Conclusion: The study also concluded that sex had no relationship with the spread of adenovirus diarrhoea among under-fives (p>0.05).
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尼日利亚南部两家三甲医院五岁以下儿童与腺病毒和轮状病毒相关腹泻的分子特征描述
背景:病毒性腹泻仍然是尼日利亚五岁以下儿童面临的一项挑战。虽然其他非病毒性腹泻病原体已被很好地记录下来,但病毒性腹泻病原体,尤其是其遗传多样性在我们当地还没有得到很好的研究,因此才有了这项研究。研究方法这是一项横断面研究,分别在贝宁市的贝宁大学教学医院(UBTH)和恩纳迪-阿齐基韦大学教学医院(NAUTH)进行。研究采用分层随机抽样法,共招募了 330 名年龄在 1 至 5 岁之间的参与者。所有腹泻粪便均使用 Quick-RNA Viral MiniPrep Kit 提取了 DNA/RNA,并保存在 -80°C 温度下进行聚合酶链反应。腺病毒的序列用 Big Dye DNA 测序试剂盒完成。生物信息学使用 R studio v3 软件中的 R 软件包完成。研究数据使用卡方进行分析,显著性水平使用 p 值(p0.05)表示。在所研究的人群中,没有检测到轮状病毒基因组。已鉴定出 10 个来自血清型 40 的腺病毒序列,并将其存入美国国家生物技术信息中心(Genbank:http://www.ncbi.nlm.nih.gov/),登录号为 ON128719 - ON128728。研究地区的腺病毒感染率为 8.7%。受感染的女性多于男性,但没有统计学意义(P>0.05)。结论研究还得出结论,性别与腺病毒腹泻在五岁以下儿童中的传播没有关系(P>0.05)。
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