IN VITRO INVESTIGATION OF ANTIOXIDANT AND ANTI-UROLITHIATIC ACTIVITY OF HOMONOIA RETUSA ROOTS

Abstract

Ayurvedic practitioners utilise Pashanabheda as an anti-urolithiatic. The root of Homonoia retusa (Euphorbiaceae), which grows in the Indian town of Tirupathi, was chosen for this study's screening for its antioxidant and anti-urolithiatic properties. The hydrogen peroxide assay (H2O2), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) techniques were utilised to assess antioxidant activity. The anti-urolithiatic activity was evaluated by nucleation and aggregation assay by adding 0.01 M sodium oxalate solutions that caused the crystallisation of Calcium oxalate. By measuring turbidity in the presence or absence of extract at 620 nm using a spectrophotometer, the effect of extract (200, 400, 600, 800, and 1000 µg/ml) was investigated. According to the result, aqueous root extract can prevent crystal growth and aggregation more than methanol-based root extract.