Identification of genetic relationships of Acacia auriculiformis clones using ISSR markers

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI:10.31276/vjst.66(2).55-59

Van Doan Duong, Thi Thu Ha Tran, Tri-Thuc Bui, Manh Tuan Nguyen, Tien Dung Nguyen

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Abstract

In this study, seven clones of Acacia auriculiformis,collected in Cam Lo district, Quang Binh province were assessed for genetic diversity using 15 ISSR markers. The results showed that seven Acacia clones had an average level of genetic variation. In which, four ISSR markers including ISSR1, ISSR3, ISSR8, and ISSR15 showed higher polymorphism information content (PIC) value than the others. The results analysis indicated that 30 out of 40 DNA fragments were polymorphic (75%, PIC=0.28-0.33). The ISSR8 exhibited the highest genetic diversity index (0.33), while ISSR15 showed the lowest diversity (0.28). Seven clones of A. auriculiformis were divided into two main groups: group I comprised four clones: Clt7, Clt18, Clt19, and Clt26; group II included the remaining three clones: Clt25, Clt43, and Clt57. Genetic variation was correlated with plant growth ability. This result exhibited that the ISSR marker was an efficient tool in assessing and selecting the good genetic resource of A. auriculiformis for the forestry tree breeding program in Vietnam.

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利用 ISSR 标记鉴定金合欢克隆的遗传关系

本研究利用 15 个 ISSR 标记评估了在广平省 Cam Lo 县采集的 7 个金合欢克隆的遗传多样性。结果表明，7 个金合欢克隆的遗传变异水平一般。其中，ISSR1、ISSR3、ISSR8 和 ISSR15 等四个 ISSR 标记的多态性信息含量（PIC）值高于其他标记。结果分析表明，40 个 DNA 片段中有 30 个具有多态性（75%，PIC=0.28-0.33）。其中 ISSR8 的遗传多样性指数最高（0.33），而 ISSR15 的多样性指数最低（0.28）。A. auriculiformis 的 7 个克隆被分为两大组：第一组由 4 个克隆组成：第 I 组包括四个克隆：Clt7、Clt18、Clt19 和 Clt26；第 II 组包括其余三个克隆：第二组包括其余三个克隆：Clt25、Clt43 和 Clt57。遗传变异与植物生长能力相关。这一结果表明，ISSR 标记是评估和筛选 A. auriculiformis 优良遗传资源的有效工具，可用于越南的林木育种计划。

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Ministry of Science and Technology, Vietnam

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