Evaluating thermotolerant sunflower genotypes with temperature induction response (TIR) technique

A. V, Lakshmi Prayaga, Sarada C, Arti Guhe
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Abstract

High temperature affects various physiological processes of the plant. Delayed sowing and changing climate both subject the crop to increasing temperatures during the crop growth period. There is a need to take on a technique to screen the wide number of genotypes for high-temperature tolerance. In the present study, a screening protocol was followed based on the principle of “acquired tolerance” in which 47 sunflower seedlings were exposed to sub-lethal heat stress to induce tolerance before subjecting to subsequent lethal stress and the second set were directly exposed to lethal stress. Significant variation was observed for the traits - survival percentage, total seedling length, and seedling weight. Tolerant inbreds were identified using Z distribution and PCA. Results suggested that TIR is a rapid and powerful technique that can be used to screen large number of germplasms to identify thermotolerant lines.
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利用温度诱导响应(TIR)技术评估耐高温向日葵基因型
高温会影响植物的各种生理过程。延迟播种和不断变化的气候都会使作物在生长期间受到不断升高的温度的影响。因此需要采用一种技术来筛选耐高温的大量基因型。在本研究中,根据 "获得性耐受 "原则制定了筛选方案,将 47 株向日葵幼苗置于亚致死热胁迫下,以诱导耐受性,然后再置于致死胁迫下,第二组幼苗则直接置于致死胁迫下。在成活率、幼苗总长度和幼苗重量等性状方面观察到显著差异。利用 Z 分布和 PCA 鉴定了耐受性近交系。结果表明,TIR 是一种快速而强大的技术,可用于筛选大量种质来鉴定耐热品系。
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