Response of gamma irradiation on germination and seedling growth of green gram var. GAM 8

Amarjeet Singh, Kalpesh V. Patel, Rajpal U. Solanki, Ramesh I. Chaudhary, Nilesh K. Chavda
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Abstract

Being a pulse crop, green gram has huge scope in crop improvement in terms of productivity and other yield related parameters. Genetic variability is a preferable option for breeders in breeding programme for varietal development programme and others crop improvement aspect. The present investigation was framed to create the variability in mung bean var. GAM 8 during the seedling period through gamma (γ) irradiation. The significant variation was observed in germination percentage and it was found that dose dependent relationship between the germination rate and dose. Significantly minimum germination percentage (22.38 %) was observed in 700 Gy but optimal lethal dose (LD50) calculated through Probit analysis based on germination percentage was revealed at 540.26 Gy. Data revealed that γ irradiation had significantly reduced the seedling growth parameters such as shoot length (10.05 cm), shoot dry weight (19.68 mg), seedling length (13.90 cm), vigour index I (311.01) and II (509.01) was registered in 700 Gy while minimum root length (3.83 cm) was occurred in 600 Gy γ irradiation. Growth reduction (GR) 50 and 30 with respect to the seedling length was occurred respectively at 1093.79 and 1469.74 Gy. Therefore, this finding as the source of genetic variability would be used in future breeding and crop improvement programme like enhancement of yield potentiality and stress management in mung bean var. GAM 8.   
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伽马辐照对青蒜变种 GAM 8 的发芽和幼苗生长的影响
作为一种脉冲作物,禾谷类作物在生产力和其他产量相关参数方面有着巨大的改良空间。遗传变异性是育种者在品种开发计划和其他作物改良方面进行育种的首选方案。本研究的目的是通过伽马(γ)辐照在绿豆变种 GAM 8 的幼苗期创造变异性。结果发现,发芽率与剂量之间存在明显的剂量依赖关系。700 Gy 的发芽率明显最低(22.38 %),但根据发芽率通过 Probit 分析计算出的最佳致死剂量(LD50)为 540.26 Gy。数据显示,γ辐照显著降低了幼苗的生长参数,如芽长(10.05 厘米)、芽干重(19.68 毫克)、苗长(13.90 厘米)、活力指数 I(311.01)和 II(509.01),而最小根长(3.83 厘米)出现在 600 Gy γ辐照下。在 1093.79 和 1469.74 Gy 的辐照下,秧苗长度分别减少了 50 和 30。因此,这一发现作为遗传变异的来源,将用于未来的育种和作物改良计划,如提高绿豆变种 GAM 8 的产量潜力和胁迫管理。
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