Development and application of transcriptomics technologies in plant science

Crop Design Pub Date : 2024-05-01 DOI:10.1016/j.cropd.2024.100057

Han Wang , Yueting Xu , Zhizhong Zhang , Guoping Zhang , Cong Tan , Lingzhen Ye

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Abstract

Over the past decade, bulk RNA sequencing (RNA-seq) has become an indispensable tool in molecular biology, and have made the novel development, with two innovative methodologies being developed, single-cell RNA sequencing (scRNA-seq) technology and spatial transcriptome (ST) technology. The scRNA-seq technology allows researchers to analyze gene expression in individual cells, providing more detailed information relative to the past technologies. Meanwhile, ST technology overcomes the limitations of single-cell sequencing in terms of loss of spatial information, enabling scientists to better understand the spatial distribution of gene expression within tissues. These advancements of transcriptomics technologies revolutionize the field of genomics and have been widely used in disease diagnosis and medicine. However, they are less utilized in plant research. This review describes the development, advantage and limitations of three transcriptomics technologies, and presents their applications in plant sciences.

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植物科学中转录组学技术的开发与应用

近十年来，大容量 RNA 测序（RNA-seq）已成为分子生物学中不可或缺的工具，并取得了新的发展，目前正在开发两种创新方法，即单细胞 RNA 测序（scRNA-seq）技术和空间转录组（ST）技术。与过去的技术相比，scRNA-seq 技术使研究人员能够分析单个细胞的基因表达，提供更详细的信息。同时，ST 技术克服了单细胞测序在空间信息丢失方面的局限性，使科学家能够更好地了解基因表达在组织内的空间分布。转录组学技术的这些进步彻底改变了基因组学领域，并已广泛应用于疾病诊断和医学领域。然而，它们在植物研究中的应用却较少。本综述介绍了三种转录组学技术的发展、优势和局限性，并介绍了它们在植物科学中的应用。

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Crop Design

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