A Review on High Performance Thin Layer Chromatography

Miss. Payal Badhe, Dr. Vijaya Barge
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Abstract

A common excellent replacement for GC and HPLC is High-Performance Thin Layer Chromatography (HPTLC), an improved and automated technique of thin-layer chromatography (TLC) that provides better separation performance and detection limits. Applications of HPTLC include the study of biological materials and phytochemicals, the measurement of herbal medications and active components, formulation fingerprinting, and the identification of adulterants in formulations. Using HPTLC, chemicals of forensic importance can be located. It is more sensitive and feasible to run many samples in a little period of time by using a small volume of solvent. It is one of the more intricate instrumental procedures, utilizing every feature available in thin-layer chromatography.
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高效薄层色谱法综述
高性能薄层色谱法(HPTLC)是气相色谱法和高效液相色谱法的常用优秀替代品,它是薄层色谱法(TLC)的一种改进型自动化技术,具有更好的分离性能和检测限。HPTLC 的应用包括研究生物材料和植物化学物质、测量中草药和活性成分、制剂指纹图谱以及鉴定制剂中的掺假物质。使用 HPTLC 可以找到对法医具有重要意义的化学物质。它的灵敏度更高,而且只需少量溶剂就能在短时间内检测大量样品。它是较为复杂的仪器程序之一,利用了薄层色谱法的所有功能。
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