Extracts of abalone intestine regulates fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae

Q3 Agricultural and Biological Sciences Fisheries and Aquatic Sciences Pub Date : 2024-02-01 DOI:10.47853/fas.2024.e13

Laxmi Sen Thakuri, C. Park, Jin Yeong Choi, Hyeon-A Kim, Han Kyu Lim, Jin Woo Park, Dong Wook Kim, D. Rhyu

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Abstract

Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210 ℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.

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鲍鱼肠道提取物可调节 3T3-L1 脂肪细胞和高脂饮食诱导的斑马鱼幼体的脂肪代谢

肥胖症是一种涉及脂肪堆积、低度炎症细胞因子释放和线粒体功能障碍等机制的疾病。本研究旨在探讨鲍鱼肠道提取物对 3T3-L1 脂肪细胞和高脂饮食诱导的斑马鱼幼体脂肪代谢的影响。在热水、乙醇和亚临界水提取物中，210 ℃亚临界水提取物（SW210）的总酚含量最高。此外，雄性鲍鱼肠的 SW210（MASW210）能最有效地控制 3T3-L1 脂肪细胞中脂质的积累和脂肪生成或脂肪生成调节因子（PPAR-γ、C/EBPα、SREBP-1c 和 FAS）的表达。同样，在喂食高脂肪的斑马鱼幼体中，MASW210 能显著抑制体重、血糖水平和脂质积累。与脂肪生成（PPAR-γ 和 C/EBPα）、脂肪生成（SREBP-1c 和 FAS）、炎症细胞因子（TNF-α 和 IL-6）、能量代谢（AMPK、lepr、SIRT1 和脂肪连通素）和线粒体生物生成（PGC-1α 和 CPT-1）相关的 mRNA 表达受 MASW210 的显著调控。这些结果表明，鲍鱼肠提取物（如 MASW210）是改善肥胖和代谢性疾病的有用生物材料。

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