ESCHERICHIA COLI RESISTANCE CAUSES COLIBACILLOSIS TO ANTIBIOTICS IN PIG FARMS IN ALOK DISTRICT SIKKA REGENCY OF NTT

Paulus Oktavianus Pyo, Hapsari Mahatmi, I. W. M. Tenaya
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Abstract

The phenomenon of antibiotic resistance is a very serious problem that needs attention and countermeasures, especially in the use of antibiotics on farms, especially in pigs. This study aimed to determine the resistance of Escherichia coli that causes colibacillosis to several types of antibiotics. The study sample was taken based on purposive sampling from pigs showing symptoms of colibacillosis.  A total of ten faecal samples were taken from a total population of 257 heads from 32 pig farms. Antibiotic resistance tests were performed using the Kirby-Bauer disc diffusion method. Results showed that all samples examined were E. coli with haemolysis γ and resistant to ampicillin, bacitracin, erythromycin (90%) and streptomycin (10%). But all isolates were sensitive to chloramphenicol, sulfamethoxazole, doxycycline and kanamycin (90%) and streptomycin (70%). The isolated E. coli showed cross-resistance to antibiotics ampicillin, bacitracin, erythromycin and streptomycin. It was concluded that E. coli was resistant to a number of antibiotics and some were even multidrug resistant. The use of antibiotics ampicillin, bacitracin, erythromycin and streptomycin in pig farms in Alok district, Sikka regency should be stopped considering that there has been resistance to isolated E. coli.  
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大肠埃希氏菌对抗生素的耐药性导致了尼泊尔特克斯和凯科斯群岛阿洛克县养猪场的大肠杆菌病
抗生素耐药性现象是一个非常严重的问题,需要引起重视并采取对策,特别是在农场使用抗生素方面,尤其是在猪身上。本研究旨在确定引起大肠杆菌病的大肠埃希氏菌对几种抗生素的耐药性。研究样本从出现大肠杆菌病症状的猪中有目的性地抽取。 共从 32 个养猪场的 257 头猪中抽取了 10 个粪便样本。抗生素耐药性测试采用柯比鲍尔盘扩散法进行。结果显示,所有受检样本均为溶血γ型大肠杆菌,对氨苄西林、杆菌肽、红霉素(90%)和链霉素(10%)耐药。但所有分离菌株都对氯霉素、磺胺甲噁唑、强力霉素、卡那霉素(90%)和链霉素(70%)敏感。分离出的大肠杆菌对抗生素氨苄西林、杆菌肽、红霉素和链霉素有交叉耐药性。结论是大肠杆菌对多种抗生素具有抗药性,有些甚至具有多重抗药性。考虑到分离出的大肠杆菌已产生抗药性,锡卡地区阿洛克县的养猪场应停止使用抗生素氨苄西林、杆菌肽、红霉素和链霉素。
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