Qualitative and quantitative phytochemical composition and antimicrobial activity of different solvent extracts of Hardwickia binata Roxb.

Abstract

Hexane and ethyl acetate extracts were employed to carry out the research on the phytochemical and antibacterial activity of Hardwickia binata Roxb. To identify the components, present in the extract, the GC-MS technique was used for analysis. Functional groups of various extracts were identified using FTIR. The plant extract in hexane and ethyl acetate was evaluated for its ability to fight human pathogenic bacteria. The results of the phytochemical screening revealed that phenols, saponins, flavonoids, cardiac glycosides, steroids and tannins are present. The GC-MS data revealed that the ethyl acetate extract contained 25 bioactive substances. The extract of ethyl acetate showed good antimicrobial activity. S. aureus (16 mm) demonstrated the largest zone of inhibition when treated with 75 μg/mL of ethyl acetate extracts. As a result, this study provides data on the phytochemical constituents identified in both extracts. As a result, it may be employed in additional therapeutic applications in near future.