首页 > 最新文献

Research Journal of Biotechnology最新文献

英文 中文
Bioprospecting of Polyhydroxy butyrate (PHB) from Bacillus thuringiensis KUMBNGBT-41 by using Agro-industrial wastes 利用农用工业废料从苏云金芽孢杆菌 KUMBNGBT-41 中提取聚羟基丁酸盐 (PHB) 的生物勘探
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt074082
G. Nandish, S. Akarsh, D. Manjunatha, H. V. Sowmya, B. Thippeswamy
The diverse group of chemicals used in making of plastic is known to be highly toxic and poses a serious threat to the biosphere. These substances besides hitting hard to ecosystem cause an array of problems like birth defects, cancer, damage of nervous and immune systems. The present research work was aimed to isolate and characterize PHB producing bacteria from landfill soil sample collected from Sanehally, Chitradurga district and qualitatively screened by using viable staining techniques. Quantitative estimation was examined by using solvent extraction method. The PHB producing bacteria were characterized based on morphological, biochemical and molecular identification and confirmed as Bacillus thuringiensis strain KUMBNGBT-41 (Accession No. MW040075). Growth parameters were optimized for biomass production using various conditions such as media-nutrient broth, incubation time-72hrs, temperature-37˚C, pH-7.0, carbon source-glucose, nitrogen source-ammonium chloride and carbon to nitrogen ratio as 4:1. The low-cost production media was used for PHB production by cheaper substrates in submerged fermentation. PHB was analysed and quantified by using UV-Visible spectrophotometer at 200-600nm.
众所周知,用于制造塑料的各种化学物质具有剧毒,对生物圈构成严重威胁。这些物质除了对生态系统造成严重破坏外,还会引发一系列问题,如先天缺陷、癌症、神经和免疫系统损伤等。本研究工作旨在从 Chitradurga 地区 Sanehally 收集的垃圾填埋场土壤样本中分离并鉴定产生 PHB 的细菌,并使用活体染色技术进行定性筛选。采用溶剂萃取法进行定量评估。根据形态学、生物化学和分子鉴定对产生 PHB 的细菌进行了表征,确认其为苏云金芽孢杆菌菌株 KUMBNGBT-41(登录号:MW040075)。在培养基-营养肉汤、培养时间-72 小时、温度-37˚C、pH-7.0、碳源-葡萄糖、氮源-氯化铵和碳氮比为 4:1 等不同条件下,对生物量生产的生长参数进行了优化。低成本生产培养基用于在浸没式发酵中使用较便宜的底物生产 PHB。使用紫外可见分光光度计在 200-600nm 波长范围内对 PHB 进行分析和定量。
{"title":"Bioprospecting of Polyhydroxy butyrate (PHB) from Bacillus thuringiensis KUMBNGBT-41 by using Agro-industrial wastes","authors":"G. Nandish, S. Akarsh, D. Manjunatha, H. V. Sowmya, B. Thippeswamy","doi":"10.25303/1904rjbt074082","DOIUrl":"https://doi.org/10.25303/1904rjbt074082","url":null,"abstract":"The diverse group of chemicals used in making of plastic is known to be highly toxic and poses a serious threat to the biosphere. These substances besides hitting hard to ecosystem cause an array of problems like birth defects, cancer, damage of nervous and immune systems. The present research work was aimed to isolate and characterize PHB producing bacteria from landfill soil sample collected from Sanehally, Chitradurga district and qualitatively screened by using viable staining techniques. Quantitative estimation was examined by using solvent extraction method. The PHB producing bacteria were characterized based on morphological, biochemical and molecular identification and confirmed as Bacillus thuringiensis strain KUMBNGBT-41 (Accession No. MW040075). Growth parameters were optimized for biomass production using various conditions such as media-nutrient broth, incubation time-72hrs, temperature-37˚C, pH-7.0, carbon source-glucose, nitrogen source-ammonium chloride and carbon to nitrogen ratio as 4:1. The low-cost production media was used for PHB production by cheaper substrates in submerged fermentation. PHB was analysed and quantified by using UV-Visible spectrophotometer at 200-600nm.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"16 9","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Titanium Dioxide Nanoparticles on Eudrilus eugeniae: A Toxicological Study 纳米二氧化钛颗粒对 Eudrilus eugeniae 的影响:毒理学研究
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt064073
Kumari P. Anitha, Y. Thangam
The increasing use of nanotechnology means that nanomaterials will inevitably enter the environment. Eco-toxicological data are therefore required so that adequate risk assessments can be carried out. Nanoparticles (NPs) exhibit greater reactivity as their sizes, less than 100nm size, are so small that their surface area is larger. The NPs can intrude into human body more easily and are more toxic than bulk material due to increased reactivity to cells in the body. Further, investigations on the ecotoxicology of NPs have also been conducted, aiming to assess NP’s harmful effects to the ecosystem. NPs of titanium dioxide (TiO2) are widely used in sun care products as well as in self-cleaning coatings. During the life cycle of these commercial products, NPs may be released into the environment and become a threat to ecosystems. Particularly, TiO2 NPs have been shown to have toxic effects and can inhibit the growth of essential soil microorganisms. This study focused on the toxicological and histopathological effects of TiO2 nanoparticles on Eudrilus eugenia, aiming to shed light on the potential hazards posed by nanoparticles to soil organisms. This study assessed the toxicity, biomass change, cocoon production and mortality rate of earthworms under various concentrations of titanium dioxide. The results showed that TiO2 nanoparticles could lead to sublethal effects on earthworm survival and reproduction and the nanoparticles were accumulated in earthworm tissues. The study emphasized the need to consider nanoparticle interactions with soil organisms.
纳米技术的应用日益广泛,这意味着纳米材料将不可避免地进入环境。因此需要生态毒理学数据,以便进行适当的风险评估。纳米粒子(NPs)的尺寸小于 100 纳米,表面积较大,因此具有更强的反应能力。由于对人体细胞的反应性更强,纳米粒子更容易侵入人体,其毒性也比块状材料更大。此外,人们还对纳米粒子的生态毒理学进行了研究,旨在评估纳米粒子对生态系统的有害影响。二氧化钛(TiO2)纳米粒子被广泛应用于防晒产品和自清洁涂料。在这些商业产品的生命周期中,氮氧化物可能会释放到环境中,对生态系统造成威胁。特别是,TiO2 NPs 已被证明具有毒性作用,并能抑制土壤中重要微生物的生长。本研究主要探讨了 TiO2 纳米粒子对 Eudrilus eugenia 的毒理学和组织病理学影响,旨在揭示纳米粒子对土壤生物的潜在危害。该研究评估了不同浓度二氧化钛对蚯蚓的毒性、生物量变化、产茧量和死亡率。结果表明,二氧化钛纳米粒子会对蚯蚓的生存和繁殖产生亚致死效应,并且纳米粒子会在蚯蚓组织中积累。该研究强调了考虑纳米粒子与土壤生物相互作用的必要性。
{"title":"Impact of Titanium Dioxide Nanoparticles on Eudrilus eugeniae: A Toxicological Study","authors":"Kumari P. Anitha, Y. Thangam","doi":"10.25303/1904rjbt064073","DOIUrl":"https://doi.org/10.25303/1904rjbt064073","url":null,"abstract":"The increasing use of nanotechnology means that nanomaterials will inevitably enter the environment. Eco-toxicological data are therefore required so that adequate risk assessments can be carried out. Nanoparticles (NPs) exhibit greater reactivity as their sizes, less than 100nm size, are so small that their surface area is larger. The NPs can intrude into human body more easily and are more toxic than bulk material due to increased reactivity to cells in the body. Further, investigations on the ecotoxicology of NPs have also been conducted, aiming to assess NP’s harmful effects to the ecosystem. NPs of titanium dioxide (TiO2) are widely used in sun care products as well as in self-cleaning coatings. During the life cycle of these commercial products, NPs may be released into the environment and become a threat to ecosystems. Particularly, TiO2 NPs have been shown to have toxic effects and can inhibit the growth of essential soil microorganisms. This study focused on the toxicological and histopathological effects of TiO2 nanoparticles on Eudrilus eugenia, aiming to shed light on the potential hazards posed by nanoparticles to soil organisms. This study assessed the toxicity, biomass change, cocoon production and mortality rate of earthworms under various concentrations of titanium dioxide. The results showed that TiO2 nanoparticles could lead to sublethal effects on earthworm survival and reproduction and the nanoparticles were accumulated in earthworm tissues. The study emphasized the need to consider nanoparticle interactions with soil organisms.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"38 5","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140411708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influential versatile potential of Entada rheedii and Myristica beddomei – an ethnobotanical approach Entada rheedii 和 Myristica beddomei 的多功能影响潜力 - 一种人种植物学方法
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt083095
Ponnusamy Subramaniam, Muthusamy Arulkumar, Soundra Rani Michael Helan, Bobby Mohammed Nazneen, E. Wesely
Aim of the study is to determine the anti-inflammatory, antidiabetic (α-amylase activity) and radical scavenging potentials of both leaf and stem ethanolic extracts of Entada rheedii and Myristica beddomei. DPPH, ABTS, H2O2, superoxide, hydroxyl and nitric oxide activity relate to inhibition ratio with different concentrations of extracts (μg/mL). Antidiabetic (α-amylase activity) and anti-inflammatory activities were also analyzed which showed high radical scavenging activity in a dose depending manner. Comparatively, leaves and stems of Entada rheedii showed higher radical scavenging activities compared to Myristica beddomei. Furthermore, both leaves and stem of Entada rheedii showed appreciable α-amylase inhibitory effects when compared with acarbose (standard drug) and also possess a strong anti-inflammatory effect (Diclofenac sodium, standard drug), compared to Myristica beddomei extracts. Overall, our results established the key evidence for Entada rheedii and Myristica beddomei extracts to be considered as a natural therapeutic aliment as the substitution for oxidative stress.
本研究旨在确定 Entada rheedii 和 Myristica beddomei 的叶和茎乙醇提取物的抗炎、抗糖尿病(α-淀粉酶活性)和自由基清除潜力。DPPH、ABTS、H2O2、超氧化物、羟基和一氧化氮活性与不同浓度提取物(μg/mL)的抑制率有关。此外,还分析了抗糖尿病(α-淀粉酶活性)和抗炎活性,结果表明它们具有很高的自由基清除活性,且活性的大小与剂量有关。与肉豆蔻相比,Entada rheedii 的叶和茎显示出更高的自由基清除活性。此外,与阿卡波糖(标准药物)相比,Entada rheedii 的叶片和茎具有明显的α-淀粉酶抑制作用;与肉豆蔻提取物相比,Entada rheedii 的叶片和茎还具有较强的抗炎作用(标准药物双氯芬酸钠)。总之,我们的研究结果为将 Entada rheedii 和 Myristica beddomei 提取物视为替代氧化应激的天然治疗食品提供了关键证据。
{"title":"Influential versatile potential of Entada rheedii and Myristica beddomei – an ethnobotanical approach","authors":"Ponnusamy Subramaniam, Muthusamy Arulkumar, Soundra Rani Michael Helan, Bobby Mohammed Nazneen, E. Wesely","doi":"10.25303/1904rjbt083095","DOIUrl":"https://doi.org/10.25303/1904rjbt083095","url":null,"abstract":"Aim of the study is to determine the anti-inflammatory, antidiabetic (α-amylase activity) and radical scavenging potentials of both leaf and stem ethanolic extracts of Entada rheedii and Myristica beddomei. DPPH, ABTS, H2O2, superoxide, hydroxyl and nitric oxide activity relate to inhibition ratio with different concentrations of extracts (μg/mL). Antidiabetic (α-amylase activity) and anti-inflammatory activities were also analyzed which showed high radical scavenging activity in a dose depending manner. Comparatively, leaves and stems of Entada rheedii showed higher radical scavenging activities compared to Myristica beddomei. Furthermore, both leaves and stem of Entada rheedii showed appreciable α-amylase inhibitory effects when compared with acarbose (standard drug) and also possess a strong anti-inflammatory effect (Diclofenac sodium, standard drug), compared to Myristica beddomei extracts. Overall, our results established the key evidence for Entada rheedii and Myristica beddomei extracts to be considered as a natural therapeutic aliment as the substitution for oxidative stress.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"69 5","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140408541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of laser bioprinting on growth characteristics of yeasts 激光生物打印对酵母生长特性的影响
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt01004
Vladimir Cheptsov, V. Zhigarkov, Irina Maximova, Nikita Minaev, Vladimir I. Yusupov
Laser-induced forward transfer (LIFT) can be helpful for bioprinting of microbial cells, but data on its impact on the microbial physiology are scarce. The aim of study was to investigate the LIFT effect on eukaryotic microorganisms (yeasts) growth characteristics. Candida albicans, Lipomyces lipofer and Saitozyma podzolica yeasts were printed on solid and liquid nutrient media and compared with traditional methods of inoculation. The laser bioprinting affects growth patterns of yeasts, leading to formation of subpopulations with different growth characteristics. The data obtained highlight necessity of studies of the LIFT effect on microorganisms’ physiology.
激光诱导正向转移(LIFT)有助于微生物细胞的生物打印,但有关其对微生物生理影响的数据却很少。本研究旨在探讨激光诱导正向转移对真核微生物(酵母菌)生长特性的影响。研究人员在固体和液体营养培养基上打印了白色念珠菌、脂肪酵母菌和荚膜酵母菌,并与传统的接种方法进行了比较。激光生物打印影响了酵母菌的生长模式,导致形成具有不同生长特性的亚群。获得的数据强调了研究激光生物打印对微生物生理影响的必要性。
{"title":"Effect of laser bioprinting on growth characteristics of yeasts","authors":"Vladimir Cheptsov, V. Zhigarkov, Irina Maximova, Nikita Minaev, Vladimir I. Yusupov","doi":"10.25303/1904rjbt01004","DOIUrl":"https://doi.org/10.25303/1904rjbt01004","url":null,"abstract":"Laser-induced forward transfer (LIFT) can be helpful for bioprinting of microbial cells, but data on its impact on the microbial physiology are scarce. The aim of study was to investigate the LIFT effect on eukaryotic microorganisms (yeasts) growth characteristics. Candida albicans, Lipomyces lipofer and Saitozyma podzolica yeasts were printed on solid and liquid nutrient media and compared with traditional methods of inoculation. The laser bioprinting affects growth patterns of yeasts, leading to formation of subpopulations with different growth characteristics. The data obtained highlight necessity of studies of the LIFT effect on microorganisms’ physiology.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"25 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140409166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prosopis juliflora invasions and ecological dominance in the sacred groves of Western Haryana, India 印度西哈里亚纳邦圣林中的糙叶木入侵和生态优势
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt040046
S. Jakhar, H. Dhiman, H. Punia, H. Saharan, Aman Boora
The present study was conducted in the sacred groves of Haryana in the semi-arid regions. Prosopis juliflora is one of the most common invasive plant species and has varying degrees of ecological consequences. In Haryana, the vast semi-arid conditions has ideally favoured the invasion of P. juliflora including the sacred groves of the State. Therefore, the present study investigated the population structure of the P. juliflora in the two sacred groves of Haryana i.e. Daya (SG-I) and Kharkada (SG-II) with different degrees of invasion. The impact of the invasive tree on the native flora was also examined by the assessment of different ecological parameters like analytical characteristics of vegetation along with four diversity indices (Shannon-Weiner Index, Simpson Index, Pielou Index and Margalef Index). The results of the study revealed that the sacred grove with low Prosopis invasion (SG-I) had high density and Basal area of native plant species as having significant values for the selected diversity indices and thus is better flourished in comparison to the highly invaded site (SG-II). It can be concluded that the native phytodiversity of the sacred groves of Haryana is in peril due to increasing P. juliflora invasion, thus efficient management strategies are required in this direction.
本研究在哈里亚纳邦半干旱地区的圣林进行。糙叶树是最常见的入侵植物物种之一,会对生态造成不同程度的影响。在哈里亚纳邦,广阔的半干旱条件非常有利于鹅掌楸的入侵,包括该邦的神圣丛林。因此,本研究调查了哈里亚纳邦两个神圣丛林园(即 Daya(SG-I)和 Kharkada(SG-II))中不同入侵程度的箭毒树种群结构。研究还通过评估不同的生态参数,如植被的分析特征以及四种多样性指数(香农-韦纳指数、辛普森指数、皮鲁指数和马加勒夫指数),考察了入侵树木对本地植物区系的影响。研究结果表明,原生植物入侵较少的圣林(SG-I)具有较高的原生植物物种密度和基部面积,在选定的多样性指数中具有显著的数值,因此与入侵严重的地点(SG-II)相比,圣林的植物生长更为茂盛。由此可以得出结论,哈里亚纳邦圣林的本地植物多样性由于箭毒榛入侵的加剧而岌岌可危,因此需要采取有效的管理策略。
{"title":"Prosopis juliflora invasions and ecological dominance in the sacred groves of Western Haryana, India","authors":"S. Jakhar, H. Dhiman, H. Punia, H. Saharan, Aman Boora","doi":"10.25303/1904rjbt040046","DOIUrl":"https://doi.org/10.25303/1904rjbt040046","url":null,"abstract":"The present study was conducted in the sacred groves of Haryana in the semi-arid regions. Prosopis juliflora is one of the most common invasive plant species and has varying degrees of ecological consequences. In Haryana, the vast semi-arid conditions has ideally favoured the invasion of P. juliflora including the sacred groves of the State. Therefore, the present study investigated the population structure of the P. juliflora in the two sacred groves of Haryana i.e. Daya (SG-I) and Kharkada (SG-II) with different degrees of invasion. The impact of the invasive tree on the native flora was also examined by the assessment of different ecological parameters like analytical characteristics of vegetation along with four diversity indices (Shannon-Weiner Index, Simpson Index, Pielou Index and Margalef Index). The results of the study revealed that the sacred grove with low Prosopis invasion (SG-I) had high density and Basal area of native plant species as having significant values for the selected diversity indices and thus is better flourished in comparison to the highly invaded site (SG-II). It can be concluded that the native phytodiversity of the sacred groves of Haryana is in peril due to increasing P. juliflora invasion, thus efficient management strategies are required in this direction.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"12 6","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Biocidal capacity of Datura (Datura stramonium L.) leaves and flowers and Cannabis (Cannabis sativa L.) seeds on some Mosquito’s larvae (Diptera: Culicidae) 曼陀罗(Datura stramonium L.)叶、花和大麻(Cannabis sativa L.)种子对某些蚊子幼虫(双翅目:蝇科)的杀生能力
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt047053
N. Basher
Plant-based alternative pesticides are safe, effective, eco-friendly and can be used instead of synthetic pesticides. The aim of this study was to evaluate the biocidal capacity of Datura stramonium leaves (DL) and flowers (DF) and Cannabis sativa seeds (CS) on Anopheles arabiensis, Culex quiquefasciatus and Aedes aegypti larvae. The selected plant parts were collected from the experimental farm, the University garden. The LC50s of A. arabinoses larvae towards the ethanol extract of each plant part were taken to estimate the single diagnostic dose for the survival and deformities tests. GC-MS analysis for the selected plant parts was investigated. The results show that DL ethanol extract detected 14 compounds of which butanol,3-methyl is the main component (79.76%) followed by toluene (6.14%). Phytol was also detected (3.9%). 14 compounds were detected from DF of which formic acid,3-methylbut-2-yl ester was the main component (82.22%) followed by dodecanoic acid, ethyl ester (3.3%) and toluene (2.86%). Also, from CS, Dronabinol (41.4%) was the main component, followed by Cannabinol (10.38%), Phytol (10.38%) and Caryophylene (2.07%). The susceptibility test of An. arabiensis larvae revealed the LC50’s of 562.95 mg/L for DL, 424.41 mg/L for DF and 175.40 mg/L for CS. It was noticed that 3-6% of An. arabiensis and 1-28% of Culex quiquefasciatus and 12-22% of Aedes aegypti larvae developed to pupae and some of them developed to adult stage after one week. In the survival test, more than 70% of the larvae were killed by a concentration supposed to kill 50% or less of the larvae. Some deformations were monitored on Anopheles, Culex and Aedes larvae including swelled body, separated alimentary canal. Field assessment should be run and the concept of LC50 should be re-written.
以植物为基础的替代杀虫剂安全、有效、环保,可用于替代合成杀虫剂。本研究旨在评估曼陀罗叶(DL)和花(DF)以及大麻籽(CS)对阿拉伯按蚊、库蚊和埃及伊蚊幼虫的杀生能力。所选植物部分取自大学花园的实验农场。阿拉伯伊蚊幼虫对每种植物乙醇提取物的半数致死浓度(LC50s)被用来估算存活和畸形试验的单一诊断剂量。对所选植物部分进行了气相色谱-质谱分析。结果显示,DL 乙醇提取物中检测到 14 种化合物,其中丁醇、3-甲基是主要成分(79.76%),其次是甲苯(6.14%)。还检测到植物醇(3.9%)。从 DF 中检测到 14 种化合物,其中甲酸 3-甲基丁-2-基酯是主要成分(82.22%),其次是十二酸乙酯(3.3%)和甲苯(2.86%)。此外,在 CS 中,屈大麻酚(41.4%)是主要成分,其次是大麻酚(10.38%)、植物醇(10.38%)和卡里芬(2.07%)。对阿拉伯蚂蚁幼虫的药敏试验显示,DL 的半致死浓度为 562.95 毫克/升,DF 为 424.41 毫克/升,CS 为 175.40 毫克/升。结果表明,3-6%的阿拉伯伊蚊、1-28%的库蚊和 12-22% 的埃及伊蚊幼虫在一周后发育成蛹,其中一些发育成成虫。在存活率测试中,超过 70% 的幼虫被浓度为 50% 或更低的杀虫剂杀死。在按蚊、库蚊和伊蚊幼虫身上监测到了一些畸形现象,包括身体膨胀、消化管分离。应进行实地评估,并重新编写半数致死浓度的概念。
{"title":"The Biocidal capacity of Datura (Datura stramonium L.) leaves and flowers and Cannabis (Cannabis sativa L.) seeds on some Mosquito’s larvae (Diptera: Culicidae)","authors":"N. Basher","doi":"10.25303/1904rjbt047053","DOIUrl":"https://doi.org/10.25303/1904rjbt047053","url":null,"abstract":"Plant-based alternative pesticides are safe, effective, eco-friendly and can be used instead of synthetic pesticides. The aim of this study was to evaluate the biocidal capacity of Datura stramonium leaves (DL) and flowers (DF) and Cannabis sativa seeds (CS) on Anopheles arabiensis, Culex quiquefasciatus and Aedes aegypti larvae. The selected plant parts were collected from the experimental farm, the University garden. The LC50s of A. arabinoses larvae towards the ethanol extract of each plant part were taken to estimate the single diagnostic dose for the survival and deformities tests. GC-MS analysis for the selected plant parts was investigated. The results show that DL ethanol extract detected 14 compounds of which butanol,3-methyl is the main component (79.76%) followed by toluene (6.14%). Phytol was also detected (3.9%). 14 compounds were detected from DF of which formic acid,3-methylbut-2-yl ester was the main component (82.22%) followed by dodecanoic acid, ethyl ester (3.3%) and toluene (2.86%). Also, from CS, Dronabinol (41.4%) was the main component, followed by Cannabinol (10.38%), Phytol (10.38%) and Caryophylene (2.07%). The susceptibility test of An. arabiensis larvae revealed the LC50’s of 562.95 mg/L for DL, 424.41 mg/L for DF and 175.40 mg/L for CS. It was noticed that 3-6% of An. arabiensis and 1-28% of Culex quiquefasciatus and 12-22% of Aedes aegypti larvae developed to pupae and some of them developed to adult stage after one week. In the survival test, more than 70% of the larvae were killed by a concentration supposed to kill 50% or less of the larvae. Some deformations were monitored on Anopheles, Culex and Aedes larvae including swelled body, separated alimentary canal. Field assessment should be run and the concept of LC50 should be re-written.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"15 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140415686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In silico Identification and Characterization of WRKY Superfamily in Capsella rubella 风疹帽菌中 WRKY 超家族的硅学鉴定和特征描述
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt1070122
Dipinte Gupta, P. Saraswat, Hunny Waswani, Sunil Kumar, Rajiv Ranjan
Plants undergo several processes throughout their life cycle such as growth, development and response to environmental stresses which require a series of signaling events regulated by transcription factors. WRKY is one of the most studied transcription factors. In this study, we analyzed 68 genes of WRKY superfamily in Capsella rubella. All the genes were studied for their phytochemical properties which include molecular weight, no. of amino acids, theoretical pI, N-glycosylation site, conserved motifs and homology modeling. A phylogenetic relationship was also established among the accessions by using MEGA6 software. The physiochemical properties revealed the no. of amino acids ranging from 149 (Probable WRKY transcription factor 45) to 1411 (disease resistance protein RRS1) while the molecular weight varies between 17.28 (Probable WRKY transcription factor 45) to 159.39 (Disease resistance protein RRS1). Based on subcellular prediction, all the proteins were predicted to be localized in the nuclear region. In addition, the 68 WRKY proteins analyzed for phylogenetic relationships had shown the existence of one major Clade and one minor Clade. In conserved domain study using web-based software tool MEME, Motif 2 was found to be highly conserved as it is present in all accessions while motif 10 was found to be least conserved i.e. found in only 4.41% accessions. Our result provides a framework for future stress related studies in Capsella rubella in the field of plant biotechnology.
植物在其整个生命周期中会经历多个过程,如生长、发育和对环境胁迫的反应,这些过程需要一系列由转录因子调控的信号事件。WRKY 是研究最多的转录因子之一。在这项研究中,我们分析了风信子中 WRKY 超家族的 68 个基因。研究了所有基因的植物化学特性,包括分子量、氨基酸数量、理论 pI、N-糖基化位点、保守基序和同源模型。此外,还利用 MEGA6 软件建立了各物种之间的系统发育关系。理化性质表明,氨基酸数目从 149(可能的 WRKY 转录因子 45)到 1411(抗病蛋白 RRS1)不等,分子量从 17.28(可能的 WRKY 转录因子 45)到 159.39(抗病蛋白 RRS1)不等。根据亚细胞预测,所有蛋白质都被预测定位于核区。此外,68 个 WRKY 蛋白的系统进化关系分析表明存在一个主要支系和一个次要支系。在使用基于网络的软件工具 MEME 进行的保守结构域研究中,发现动点 2 是高度保守的,因为它存在于所有品种中,而动点 10 则是最不保守的,即仅在 4.41% 的品种中发现。我们的研究结果为今后在植物生物技术领域开展风信子胁迫相关研究提供了一个框架。
{"title":"In silico Identification and Characterization of WRKY Superfamily in Capsella rubella","authors":"Dipinte Gupta, P. Saraswat, Hunny Waswani, Sunil Kumar, Rajiv Ranjan","doi":"10.25303/1904rjbt1070122","DOIUrl":"https://doi.org/10.25303/1904rjbt1070122","url":null,"abstract":"Plants undergo several processes throughout their life cycle such as growth, development and response to environmental stresses which require a series of signaling events regulated by transcription factors. WRKY is one of the most studied transcription factors. In this study, we analyzed 68 genes of WRKY superfamily in Capsella rubella. All the genes were studied for their phytochemical properties which include molecular weight, no. of amino acids, theoretical pI, N-glycosylation site, conserved motifs and homology modeling. A phylogenetic relationship was also established among the accessions by using MEGA6 software. The physiochemical properties revealed the no. of amino acids ranging from 149 (Probable WRKY transcription factor 45) to 1411 (disease resistance protein RRS1) while the molecular weight varies between 17.28 (Probable WRKY transcription factor 45) to 159.39 (Disease resistance protein RRS1). Based on subcellular prediction, all the proteins were predicted to be localized in the nuclear region. In addition, the 68 WRKY proteins analyzed for phylogenetic relationships had shown the existence of one major Clade and one minor Clade. In conserved domain study using web-based software tool MEME, Motif 2 was found to be highly conserved as it is present in all accessions while motif 10 was found to be least conserved i.e. found in only 4.41% accessions. Our result provides a framework for future stress related studies in Capsella rubella in the field of plant biotechnology.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"15 6","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of antioxidant activity, chlorophyll and carotenoids content of Kappaphycus alvarezii: Comparison of seaweed with traditional Chinese Medicines 测定阿尔瓦雷斯海藻的抗氧化活性、叶绿素和类胡萝卜素含量:海藻与传统中药的比较
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt024031
Leong Z.L. Regina, Kiung Tai Fhong Sharon, Lai Huat Lim, Shao Feng Su, S. Teo
After the pandemic of COVID-19, there has been a growing interest in food safety concerns. Besides consuming wild-type animals, another focus is the synthetic compounds used in food preparation. Various reports show that antioxidant mechanisms might be closely related to decreasing the risk of disease infection. On 18 March 2020, Malaysia had the first Movement Control Order (MCO) that all communities must stay home. Public also questions whether natural food, mainly traditional Chinese medicine, can act as an antioxidant agent. Therefore, spectrophotometric procedures for determining the antioxidant capacities of selected samples were conducted. This method is based on scavenging 2, 2 diphenyl 1-picryl hydroxyl (DPPH) by adding a radical species or an antioxidant that decolourises the DPPH solution. This study aimed to compare, using the DPPH assay, the antioxidant level of Kappaphycus alvarezii to other traditional Chinese medicines (TCM) such as Andrographis paniculata, Lycium chinense and Ziziphus jujube. This study characterises the antioxidant activity of traditional Chinese medicines associated with carotenoid and total chlorophyll content from seaweed. Antioxidant analysis has shown that the highest antioxidant property was observed in the solvent extract of L. chinense. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants in selected samples.
COVID-19 大流行之后,人们对食品安全问题的关注与日俱增。除了食用野生动物外,另一个关注点是食品制作过程中使用的合成化合物。各种报告显示,抗氧化机制可能与降低疾病感染风险密切相关。2020 年 3 月 18 日,马来西亚发布了首个 "运动控制令"(MCO),要求所有社区必须留在家中。公众还质疑以中药为主的天然食品是否能起到抗氧化剂的作用。因此,研究人员采用分光光度法测定了部分样本的抗氧化能力。这种方法是通过加入能使 DPPH 溶液脱色的自由基或抗氧化剂来清除 2,2-二苯基-1-吡啶羟基(DPPH)。本研究的目的是利用 DPPH 法,比较白花蛇舌草与穿心莲、枸杞子和酸枣仁等其他传统中药的抗氧化水平。本研究分析了与海藻中类胡萝卜素和总叶绿素含量相关的中药抗氧化活性。抗氧化分析表明,枸杞的溶剂提取物具有最高的抗氧化性。DPPH 分析法为评估所选样品中潜在的抗氧化剂提供了一种简便、快速的方法。
{"title":"Determination of antioxidant activity, chlorophyll and carotenoids content of Kappaphycus alvarezii: Comparison of seaweed with traditional Chinese Medicines","authors":"Leong Z.L. Regina, Kiung Tai Fhong Sharon, Lai Huat Lim, Shao Feng Su, S. Teo","doi":"10.25303/1904rjbt024031","DOIUrl":"https://doi.org/10.25303/1904rjbt024031","url":null,"abstract":"After the pandemic of COVID-19, there has been a growing interest in food safety concerns. Besides consuming wild-type animals, another focus is the synthetic compounds used in food preparation. Various reports show that antioxidant mechanisms might be closely related to decreasing the risk of disease infection. On 18 March 2020, Malaysia had the first Movement Control Order (MCO) that all communities must stay home. Public also questions whether natural food, mainly traditional Chinese medicine, can act as an antioxidant agent. Therefore, spectrophotometric procedures for determining the antioxidant capacities of selected samples were conducted. This method is based on scavenging 2, 2 diphenyl 1-picryl hydroxyl (DPPH) by adding a radical species or an antioxidant that decolourises the DPPH solution. This study aimed to compare, using the DPPH assay, the antioxidant level of Kappaphycus alvarezii to other traditional Chinese medicines (TCM) such as Andrographis paniculata, Lycium chinense and Ziziphus jujube. This study characterises the antioxidant activity of traditional Chinese medicines associated with carotenoid and total chlorophyll content from seaweed. Antioxidant analysis has shown that the highest antioxidant property was observed in the solvent extract of L. chinense. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants in selected samples.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"26 8","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Safety and Efficacy of Temozolomide Combinations in Glioblastoma Patients; A Meta-Analysis 替莫唑胺联合用药对胶质母细胞瘤患者的安全性和疗效;Meta 分析
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt1230134
R.B. Khan, M.K. Verma, A. Tiwari, A. Siddiqui, R. Chowdhary
Globally, glioblastoma is the fetal brain tumor with the highest incidence. Patients with glioblastoma who have received a clinical diagnosis need combination therapy, which combines radiation and chemotherapy drugs that have shown promising benefits. The treatment of choice for glioblastoma, both alone and in combination, is temozolomide. Studies have shown that temozolomide combined with other medications and radiotherapy in the management of glioblastoma is effective in reducing tumor size, preventing recurrence and limiting tumor progression. The study also emphasizes the safety profile of temozolomide combinations and pathophysiology of tumor, size, growth and reoccurrence. For the meta-analysis, a detailed search of scientific literature was conducted utilizing the most relevant scientific studies published to date on the intervention of temozolomide combinations to manage Glioblastoma. A search was conducted across a number of databases including Scielo, Cochrane Central Register of Controlled Trials and PubMed. The MedCalC tool was used to do a meta-analysis in accordance with Prisma standards for odds ratio between studies, risk factor analysis and relative risk. The Cochrane Central Register of Controlled Trials, Scielo and other databases were used to retrieve a total of 1635 studies for the current analysis. The meta-analysis with three distinct parameters odds ratio, risk difference and relative risk was significant with p value <0.001 (for TMZ with other chemotherapeutic agents) and p value <0.003 (for TMZ with radiation therapy). The 95% Cl for TMZ with other chemotherapeutic agents was higher for odds ratio (2.539 to 3.466), risk difference (1.345 to 1.669) and relative risk (0.192 to 0.280) over TMZ with radiation therapy (Odds ratio 1.051 to 1.327; Risk difference 0.0116 to 0.0623 and Relative risk 1.016 to 1.100). When treating GBM, TMZ in combination with other chemotherapeutic drugs has been found to be more successful than TMZ alone. Clinical trials based on TMZ offer GBM patients with freshly diagnosed solid tumors a better survival rate.
在全球范围内,胶质母细胞瘤是发病率最高的胎儿脑肿瘤。得到临床诊断的胶质母细胞瘤患者需要接受综合治疗,这种治疗将放疗和化疗药物结合在一起,并显示出良好的疗效。治疗胶质母细胞瘤的首选药物是替莫唑胺,无论是单独使用还是联合使用。研究表明,替莫唑胺与其他药物和放疗相结合治疗胶质母细胞瘤,能有效缩小肿瘤体积、预防复发和限制肿瘤进展。研究还强调了替莫唑胺联合用药的安全性,以及肿瘤、大小、生长和复发的病理生理学。为了进行荟萃分析,我们利用迄今为止发表的与替莫唑胺联合用药治疗胶质母细胞瘤最相关的科学研究,对科学文献进行了详细的检索。检索涉及多个数据库,包括 Scielo、Cochrane Central Register of Controlled Trials 和 PubMed。使用 MedCalC 工具按照 Prisma 标准对研究之间的几率比、风险因素分析和相对风险进行了荟萃分析。本次分析共使用 Cochrane 对照试验中央登记册、Scielo 和其他数据库检索了 1635 项研究。荟萃分析的三个不同参数几率比、风险差异和相对风险均具有显著性,P值小于0.001(TMZ与其他化疗药物的比较)和P值小于0.003(TMZ与放射治疗的比较)。TMZ联合其他化疗药物的几率比(2.539~3.466)、风险差(1.345~1.669)和相对风险(0.192~0.280)的95% Cl值均高于TMZ联合放疗(几率比1.051~1.327;风险差0.0116~0.0623;相对风险1.016~1.100)。在治疗 GBM 时,发现 TMZ 与其他化疗药物联合使用比单独使用 TMZ 更为成功。基于 TMZ 的临床试验为新确诊的实体瘤 GBM 患者提供了更高的生存率。
{"title":"Safety and Efficacy of Temozolomide Combinations in Glioblastoma Patients; A Meta-Analysis","authors":"R.B. Khan, M.K. Verma, A. Tiwari, A. Siddiqui, R. Chowdhary","doi":"10.25303/1904rjbt1230134","DOIUrl":"https://doi.org/10.25303/1904rjbt1230134","url":null,"abstract":"Globally, glioblastoma is the fetal brain tumor with the highest incidence. Patients with glioblastoma who have received a clinical diagnosis need combination therapy, which combines radiation and chemotherapy drugs that have shown promising benefits. The treatment of choice for glioblastoma, both alone and in combination, is temozolomide. Studies have shown that temozolomide combined with other medications and radiotherapy in the management of glioblastoma is effective in reducing tumor size, preventing recurrence and limiting tumor progression. The study also emphasizes the safety profile of temozolomide combinations and pathophysiology of tumor, size, growth and reoccurrence. For the meta-analysis, a detailed search of scientific literature was conducted utilizing the most relevant scientific studies published to date on the intervention of temozolomide combinations to manage Glioblastoma. A search was conducted across a number of databases including Scielo, Cochrane Central Register of Controlled Trials and PubMed. The MedCalC tool was used to do a meta-analysis in accordance with Prisma standards for odds ratio between studies, risk factor analysis and relative risk. The Cochrane Central Register of Controlled Trials, Scielo and other databases were used to retrieve a total of 1635 studies for the current analysis. The meta-analysis with three distinct parameters odds ratio, risk difference and relative risk was significant with p value <0.001 (for TMZ with other chemotherapeutic agents) and p value <0.003 (for TMZ with radiation therapy). The 95% Cl for TMZ with other chemotherapeutic agents was higher for odds ratio (2.539 to 3.466), risk difference (1.345 to 1.669) and relative risk (0.192 to 0.280) over TMZ with radiation therapy (Odds ratio 1.051 to 1.327; Risk difference 0.0116 to 0.0623 and Relative risk 1.016 to 1.100). When treating GBM, TMZ in combination with other chemotherapeutic drugs has been found to be more successful than TMZ alone. Clinical trials based on TMZ offer GBM patients with freshly diagnosed solid tumors a better survival rate.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"2020 24","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140416185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Identification of Biofilm forming Bacteria from Glass Panels exposed in Sea Waters of South Andaman, India 从暴露在印度南安达曼海水中的玻璃板上分离和鉴定形成生物膜的细菌
IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt054063
Shehin M.C. Sadaka, K.A. Jayaraj, Ameen Ummath, Venu Sasidharan, Fahmeeda P.S. Parveen
Marine organisms attach to surfaces that are submerged in seawater in a process known as biofouling. These organisms can colonize on various surfaces such as ship hulls, pillars, jetties, undersea pipelines, oil rigs and buoys. Foulers can include bacteria, algae, barnacles, mussels and other invertebrates. The initial adhesion of fouling bacteria is the first stage of biofouling. The growth and attachment of marine fouling bacteria are influenced by various environmental factors including salinity, temperature and nutrient availability. The bacteria forming marine biofilm were isolated and identified from glass panels. Basic microbiological phenotypic identification methods and genotypic validation using 16s rRNA gene sequencing were used to identify the bacterial strains. The phylogenetic analysis of the 16s rRNA gene sequences confirmed 13 different species (Bacillus aquimaris (2), Vibrio hepatarius (1), Exiguobacterium profundum (3), Exiguobacterium aestuarii (2), Vibrio parahaemolyticus (2), Vibrio alginolyticus (3), Vibrio azureus (2), Vibrio fluvialis (2), Priestia flexa (1), Pseudomonas aeruginosa (2), Bacillus megaterium (2), Lysinibacillus macroides (2) and Bacillus subtilis (1)) of fouling bacteria belonging to six genera.
海洋生物附着在浸没在海水中的表面,这一过程被称为生物污损。这些生物可在船体、支柱、防波堤、海底管道、石油钻井平台和浮标等各种表面上定居。污损生物包括细菌、藻类、藤壶、贻贝和其他无脊椎动物。污损细菌的最初附着是生物污损的第一阶段。海洋污损细菌的生长和附着受各种环境因素的影响,包括盐度、温度和营养供应。从玻璃板上分离并鉴定了形成海洋生物薄膜的细菌。采用基本的微生物表型鉴定方法和 16s rRNA 基因测序的基因型验证方法来鉴定细菌菌株。藻溶性弧菌 (3)、天蓝色弧菌 (2)、河水弧菌 (2)、柔藻弧菌 (1)、铜绿假单胞菌 (2)、巨大芽孢杆菌 (2)、大肠溶血芽孢杆菌 (2) 和枯草芽孢杆菌 (1))。
{"title":"Isolation and Identification of Biofilm forming Bacteria from Glass Panels exposed in Sea Waters of South Andaman, India","authors":"Shehin M.C. Sadaka, K.A. Jayaraj, Ameen Ummath, Venu Sasidharan, Fahmeeda P.S. Parveen","doi":"10.25303/1904rjbt054063","DOIUrl":"https://doi.org/10.25303/1904rjbt054063","url":null,"abstract":"Marine organisms attach to surfaces that are submerged in seawater in a process known as biofouling. These organisms can colonize on various surfaces such as ship hulls, pillars, jetties, undersea pipelines, oil rigs and buoys. Foulers can include bacteria, algae, barnacles, mussels and other invertebrates. The initial adhesion of fouling bacteria is the first stage of biofouling. The growth and attachment of marine fouling bacteria are influenced by various environmental factors including salinity, temperature and nutrient availability. The bacteria forming marine biofilm were isolated and identified from glass panels. Basic microbiological phenotypic identification methods and genotypic validation using 16s rRNA gene sequencing were used to identify the bacterial strains. The phylogenetic analysis of the 16s rRNA gene sequences confirmed 13 different species (Bacillus aquimaris (2), Vibrio hepatarius (1), Exiguobacterium profundum (3), Exiguobacterium aestuarii (2), Vibrio parahaemolyticus (2), Vibrio alginolyticus (3), Vibrio azureus (2), Vibrio fluvialis (2), Priestia flexa (1), Pseudomonas aeruginosa (2), Bacillus megaterium (2), Lysinibacillus macroides (2) and Bacillus subtilis (1)) of fouling bacteria belonging to six genera.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"21 3","pages":""},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140412369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Research Journal of Biotechnology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1