Detection Of Sm Gene Resistance To Gray Leaf Spot In (Stemphylium Spp.) Tomato Cultivars Iraq

Q3 Agricultural and Biological Sciences Comunicata Scientiae Pub Date : 2024-01-31 DOI:10.14295/cs.v15.3564
Zeina Thamer Abd ULHussein Al Rufaye, Warqaa Muhammed Shariff AL Sheikh, Intisar Kadhum Ghaleb
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Abstract

Abstract Gray leaf spot, Occurs due to Stemphylium spp., is a foliar disease in tomato. The Resistance against gray leaf spot disease is conferred by a single incompletely dominant gene Sm located on chromosome 11. This study aimed to identify cultivar resistant alleles or susceptible alleles by molecular marker tightly linked to the Sm gene and the use of marker-assisted selection inbreeding. In this study, we used eight tomato cultivars Farmed in Iraq. The analysis demonstrated that the co-dominant marker Sm-InDel, which produced 122-bp fragment for resistance in seven genotypes and a 140-bp fragment for susceptible alleles in one genotype, respectively could be utilized in Marker-assisted selection (MAS)for gray leaf spot resistance.
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伊拉克番茄栽培品种对灰叶斑病的 Sm 基因抗性检测
摘要 灰叶斑病(Stemphylium spp.引起)是番茄的一种叶部病害。番茄对灰叶斑病的抗性是由位于第 11 号染色体上的单个不完全显性基因 Sm 遗传的。本研究旨在通过与 Sm 基因紧密相连的分子标记,并利用标记辅助近交选择法,鉴定抗病等位基因或易感等位基因。在这项研究中,我们使用了 8 个在伊拉克种植的番茄品种。分析表明,共显性标记 Sm-InDel 在 7 个基因型中产生 122-bp 的抗性片段,在 1 个基因型中产生 140-bp 的易感等位基因片段,该标记可用于灰色叶斑病抗性的标记辅助选择(MAS)。
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来源期刊
Comunicata Scientiae
Comunicata Scientiae Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
0.90
自引率
0.00%
发文量
78
期刊介绍: The Comunicata Scientiae journal edited by Campus “Profa Cinobelina Elvas” of Federal University of Piauí (Brazil) publishes original articles, scientific notes and review articles (when invited by the editorial board), which present significant importance for Agrarian and Environmental Sciences in Portuguese, Spanish and preferentially, in English.
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