Purification of stilbene synthase from Phaseolus vulgaris and determination of its catalytic activity

Abstract

Stilbene compounds, secondary metabolites produced by plants in response to external environmental stimuli, are garnering increasing attention. Stilbene synthase (STS) plays a crucial role in their synthesis. This study focuses on the purification of stilbene synthase from Phaseolus vulgaris and the determination of its catalytic activity. The stilbene synthase gene obtained from the Phaseolus genome was expressed in large quantities by constructing engineered strains of Escherichia coli . Furthermore, the active proteins produced by the stilbene synthase gene in Phaseolus plants were measured by assessing the activity of stilbene synthase obtained from both His column and Q column purifications. In this study, the active stilbene synthase gene was initially identified from the Papilionoideae. Exploring stilbene synthase contributes to a deeper understanding of the relationship between plant stress resistance and plant evolution.