Chen Bo, Tahir Muhammad, Fatima, Zafar Sara, Li Hui, Li Jia, J. R. Arthur, Caiming Gou, M. A. Hayssam, Abbas Manzar
An efficient callogenesis and regeneration protocol was optimized for six different wheat (Triticum aestivum L . ) varieties including Chakwal-50, Galaxy-01, NARC-09, Pakistan-13, Millat-11
针对六种不同的小麦（Triticum aestivum L .
{"title":"Establishment of an efficient regeneration protocol of six different varieties of wheat (Triticum aestivum L.)","authors":"Chen Bo, Tahir Muhammad, Fatima, Zafar Sara, Li Hui, Li Jia, J. R. Arthur, Caiming Gou, M. A. Hayssam, Abbas Manzar","doi":"10.5897/ajb2023.17638","DOIUrl":"https://doi.org/10.5897/ajb2023.17638","url":null,"abstract":"An efficient callogenesis and regeneration protocol was optimized for six different wheat (Triticum aestivum L . ) varieties including Chakwal-50, Galaxy-01, NARC-09, Pakistan-13, Millat-11","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"26 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140412401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Likiti Kola Olivier, Kisuka Muvirirwa Rosine, Diko Eboma Guy-Roger, Bulonza Mulimbanya Jean-Claude, Bakelena Zeyimo Tony, Mubenga Kankonda Onésime, M. Godefroid
{"title":"Distribution of cassava mosaic begomoviruses in the North-Western provinces of Democratic Republic of Congo","authors":"Likiti Kola Olivier, Kisuka Muvirirwa Rosine, Diko Eboma Guy-Roger, Bulonza Mulimbanya Jean-Claude, Bakelena Zeyimo Tony, Mubenga Kankonda Onésime, M. Godefroid","doi":"10.5897/ajb2023.17634","DOIUrl":"https://doi.org/10.5897/ajb2023.17634","url":null,"abstract":"","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"21 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yves Patrik Bouniounou Damis, Mètuor-Dabiré Amana, Yasmine Tiemtore Rahimatou, Sougue Serge, Rose Bonkoungou Pegdwende, S. Jacques
The growing prevalence of Imipenemase (IMP) metallo-beta-lactamases (MBL) producing strains of Gram-negative bacilli (GNB) pathogens is a real concern for clinicians in the light of therapeutic impasses driven. However, resistance genes encoding these enzymes are hardly documented in Burkina Faso. This study aims to show carbapenem-resistance mediated by the production of IMP-type carbapenemase in GNB clinical strains collected in Ouagadougou, Burkina Faso. Strains resistance profile to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined by the disk diffusion method. Classical polymerase chain reaction (PCR) was carried out to detect β-lactamase ( bla ) gene of IMP in resistant strains. Out of 158 GNB collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or to aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (45.1%; n=41) and Klebsiella pneumoniae (26.5% n=24). Among 32 resistant strains bla IMP gene positive (35.2%), Escherichia coli was the predominant species carrying resistance gene (18.7%, n=17/91). The findings strengthen the scarce existing scientific data on antimicrobial resistance mediated by metallo-beta-lactamases (MBL) in Burkina Faso.
{"title":"Multi-resistance to carbapenems by the production of Imipenemase (IMP)-types carbapenemases in Gram-negative bacilli in Burkina Faso","authors":"Yves Patrik Bouniounou Damis, Mètuor-Dabiré Amana, Yasmine Tiemtore Rahimatou, Sougue Serge, Rose Bonkoungou Pegdwende, S. Jacques","doi":"10.5897/ajb2023.17626","DOIUrl":"https://doi.org/10.5897/ajb2023.17626","url":null,"abstract":"The growing prevalence of Imipenemase (IMP) metallo-beta-lactamases (MBL) producing strains of Gram-negative bacilli (GNB) pathogens is a real concern for clinicians in the light of therapeutic impasses driven. However, resistance genes encoding these enzymes are hardly documented in Burkina Faso. This study aims to show carbapenem-resistance mediated by the production of IMP-type carbapenemase in GNB clinical strains collected in Ouagadougou, Burkina Faso. Strains resistance profile to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined by the disk diffusion method. Classical polymerase chain reaction (PCR) was carried out to detect β-lactamase ( bla ) gene of IMP in resistant strains. Out of 158 GNB collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or to aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (45.1%; n=41) and Klebsiella pneumoniae (26.5% n=24). Among 32 resistant strains bla IMP gene positive (35.2%), Escherichia coli was the predominant species carrying resistance gene (18.7%, n=17/91). The findings strengthen the scarce existing scientific data on antimicrobial resistance mediated by metallo-beta-lactamases (MBL) in Burkina Faso.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"33 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140412315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimates of genetic variability in ultraviolet irradiated populations of summer squash","authors":"I. K. Mervat","doi":"10.5897/ajb2023.17624","DOIUrl":"https://doi.org/10.5897/ajb2023.17624","url":null,"abstract":"","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"701 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140476578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HbA1c is the best indicator for monitoring glycemic control in both type 1 and type 2 diabetic patients. Its value allows us to predict the development of long-term degenerative complications of diabetes. Several techniques offered by different laboratories allow the determination of HbA1c based on different principles. The purpose of the present study was to perform the verification of the analytical performances of the Selectra Pro M automated system, using immunoturbidimetry as the HbA1c assay method. Risk assessment of the method was performed; the analytical performances of the assay process was evaluated and the immunoturbidimetric method on the Selectra Pro M was compared to the capillary method on the Capillarys 2 Flex Piercing. Reproducibility and intermediate precision were satisfactory with CV in the range of 1.78 to 1.96% for the low level of Internal Quality Control (IQC) (HbA1c = 5.4%) and 0.82 to 2.28% for the high level of IQC (HbA1c = 11.3%). The reagent was linear from 2.5 to 16% (4 to 151 mmol/mol). The accuracy was considered satisfactory. The linear regression calculation (least squares line) showed an excellent correlation (R 2 = 0.96) between the two techniques with an equation of the type [Capillarys] = 1.13 [Selectra Pro M] - 0.83. All documented and tested performances correspond to the performance required for HbA1c assay at INH of Lomé.
{"title":"Analytical performances description of the immunoturbidimetric method for the determination of HbA1c using Selectra Pro M automated system at the Institut National dHygine (INH) of Lom","authors":"Katawa Gnatoulma, Adjete Kossi, Marthe Amessoudji Oukoe, Halatoko Wemboo, Edlom Tchadie Pélagie, Bara Fagdeba, Dossou Irène, Nguepou Tchopba Christèle, Simplice Karou Damintoti, Ameyapoh Yovi","doi":"10.5897/ajb2023.17604","DOIUrl":"https://doi.org/10.5897/ajb2023.17604","url":null,"abstract":"HbA1c is the best indicator for monitoring glycemic control in both type 1 and type 2 diabetic patients. Its value allows us to predict the development of long-term degenerative complications of diabetes. Several techniques offered by different laboratories allow the determination of HbA1c based on different principles. The purpose of the present study was to perform the verification of the analytical performances of the Selectra Pro M automated system, using immunoturbidimetry as the HbA1c assay method. Risk assessment of the method was performed; the analytical performances of the assay process was evaluated and the immunoturbidimetric method on the Selectra Pro M was compared to the capillary method on the Capillarys 2 Flex Piercing. Reproducibility and intermediate precision were satisfactory with CV in the range of 1.78 to 1.96% for the low level of Internal Quality Control (IQC) (HbA1c = 5.4%) and 0.82 to 2.28% for the high level of IQC (HbA1c = 11.3%). The reagent was linear from 2.5 to 16% (4 to 151 mmol/mol). The accuracy was considered satisfactory. The linear regression calculation (least squares line) showed an excellent correlation (R 2 = 0.96) between the two techniques with an equation of the type [Capillarys] = 1.13 [Selectra Pro M] - 0.83. All documented and tested performances correspond to the performance required for HbA1c assay at INH of Lomé.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"480 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stilbene compounds, secondary metabolites produced by plants in response to external environmental stimuli, are garnering increasing attention. Stilbene synthase (STS) plays a crucial role in their synthesis. This study focuses on the purification of stilbene synthase from Phaseolus vulgaris and the determination of its catalytic activity. The stilbene synthase gene obtained from the Phaseolus genome was expressed in large quantities by constructing engineered strains of Escherichia coli . Furthermore, the active proteins produced by the stilbene synthase gene in Phaseolus plants were measured by assessing the activity of stilbene synthase obtained from both His column and Q column purifications. In this study, the active stilbene synthase gene was initially identified from the Papilionoideae. Exploring stilbene synthase contributes to a deeper understanding of the relationship between plant stress resistance and plant evolution.
芪类化合物是植物在外界环境刺激下产生的次级代谢产物，越来越受到人们的关注。二苯乙烯合成酶（STS）在其合成过程中起着至关重要的作用。本研究的重点是从粗壮黄 Phaseolus 植物中纯化二苯乙烯合成酶并测定其催化活性。通过构建大肠杆菌工程菌株，大量表达了从毛蕊花基因组中获得的二苯乙烯合成酶基因。此外，通过评估 His 柱和 Q 柱纯化得到的二苯乙烯合成酶的活性，测定了二苯乙烯合成酶基因在毛蕊花植物中产生的活性蛋白。在这项研究中，从木犀科植物中初步鉴定了具有活性的二苯乙烯合成酶基因。对二苯乙烯合成酶的探索有助于深入了解植物抗逆性与植物进化之间的关系。
{"title":"Purification of stilbene synthase from Phaseolus vulgaris and determination of its catalytic activity","authors":"Yongkang Yan, Zhanyou Yan, Guofang Zhao","doi":"10.5897/ajb2023.17629","DOIUrl":"https://doi.org/10.5897/ajb2023.17629","url":null,"abstract":"Stilbene compounds, secondary metabolites produced by plants in response to external environmental stimuli, are garnering increasing attention. Stilbene synthase (STS) plays a crucial role in their synthesis. This study focuses on the purification of stilbene synthase from Phaseolus vulgaris and the determination of its catalytic activity. The stilbene synthase gene obtained from the Phaseolus genome was expressed in large quantities by constructing engineered strains of Escherichia coli . Furthermore, the active proteins produced by the stilbene synthase gene in Phaseolus plants were measured by assessing the activity of stilbene synthase obtained from both His column and Q column purifications. In this study, the active stilbene synthase gene was initially identified from the Papilionoideae. Exploring stilbene synthase contributes to a deeper understanding of the relationship between plant stress resistance and plant evolution.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"71 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140479049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khady Doumbia, Yaya Nadiala Coulibaly, Bi Irié Roger Djè, Yao Raymond Koffi, Lassana Tioté, K. K. Koffi
The genetic diversity of a genetic resource is an indicator of its richness and offers many possibilities for improvement. Cowpea is an important source of nutrients for humans and animals. This study aimed at assessing genetic diversity and relationships present among 135 accessions in the Centre National de Recherches Agronomiques (CNRA) collection, using qualitative traits. Traits analysis revealed significant genetic variation between the studied accessions through high Shannon diversity index. Estimated for 15 morphological characters with 0.80 mean value, H’ ranged from 0.55 to 1. Seed traits generally expressed mean high diversity index (0.70-0.89). Between 15 parameters assessed, 12 exhibited the most discriminating variability of collection. The three MCA factorial axes explained 19.085% of phenotypic variability. The trait seed colour being correlated to these axes could be used as a selection criterion for local cowpeas improvement. Based on MCA and HAC methods, these accessions were classified into three distinct groups. Containing most accessions, subgroup Ia includes all seeds, pod, and flower colours. Almost all accessions of subgroup Ib possessed cream-coloured, smooth and globose seeds. All cluster II accessions have a creeping habit. The CNRA collection being very diversified, it constitutes a useful resource for future cowpea improvement studies in Côte d'Ivoire.
{"title":"Morphological traits variation of cowpea (Vigna unguiculata L. Walp) grown in Cte dIvoire","authors":"Khady Doumbia, Yaya Nadiala Coulibaly, Bi Irié Roger Djè, Yao Raymond Koffi, Lassana Tioté, K. K. Koffi","doi":"10.5897/ajb2023.17623","DOIUrl":"https://doi.org/10.5897/ajb2023.17623","url":null,"abstract":"The genetic diversity of a genetic resource is an indicator of its richness and offers many possibilities for improvement. Cowpea is an important source of nutrients for humans and animals. This study aimed at assessing genetic diversity and relationships present among 135 accessions in the Centre National de Recherches Agronomiques (CNRA) collection, using qualitative traits. Traits analysis revealed significant genetic variation between the studied accessions through high Shannon diversity index. Estimated for 15 morphological characters with 0.80 mean value, H’ ranged from 0.55 to 1. Seed traits generally expressed mean high diversity index (0.70-0.89). Between 15 parameters assessed, 12 exhibited the most discriminating variability of collection. The three MCA factorial axes explained 19.085% of phenotypic variability. The trait seed colour being correlated to these axes could be used as a selection criterion for local cowpeas improvement. Based on MCA and HAC methods, these accessions were classified into three distinct groups. Containing most accessions, subgroup Ia includes all seeds, pod, and flower colours. Almost all accessions of subgroup Ib possessed cream-coloured, smooth and globose seeds. All cluster II accessions have a creeping habit. The CNRA collection being very diversified, it constitutes a useful resource for future cowpea improvement studies in Côte d'Ivoire.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"483 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Poh Konan Georges N’goran, E. Minyaka, K. M. Kouassi, Siaka Kone, N’dede Theodore Djeni, Christophe Kouamé, J. N’Zi
This study aims to identify the suitable subculturing time, culture media (protocols) and explants type suitable for somatic embryogenesis expression in five elite genotypes of Theobroma cacao L. Thus, petals and staminodes explants extracted from immature flower buds of genotypes (C1, C8, C9, C14, and C16) are first grown in different media (CIM and CDM) to assess the subculturing time. Then, successive transfers in EDM medium at every 4 weeks intervals until 24 weeks. To assess culture media preference, these genotypes were grown in four different protocols (I, II, III, and IV) for callus induction. They were then successively subcultured (four times at 4 weeks intervals) into EDM. About the subculture time, embryo expression is earlier in C1, C9, C14, and C16 genotypes (2nd and 4th weeks) than C8 genotype (12 and 16th weeks). However, C1, C14, and C16 (with staminodes explants) prefer protocol IV, while their petal explants prefer protocol II. Also, C9 staminodes explants prefer protocol III while the C9 and C8 petals explants prefer protocol I. This study has identified suitable explants and protocols for somatic embryo production in the tested genotypes. Moreover, this work has laid out a new strategy to overcome recalcitrance to the somatic embryogenesis of T. cacao genotypes.
{"title":"Preferential expression of somatic embryogenesis in five elite genotypes of Theobroma cacao (L.) associated with explant type and protocols used","authors":"Poh Konan Georges N’goran, E. Minyaka, K. M. Kouassi, Siaka Kone, N’dede Theodore Djeni, Christophe Kouamé, J. N’Zi","doi":"10.5897/ajb2023.17568","DOIUrl":"https://doi.org/10.5897/ajb2023.17568","url":null,"abstract":"This study aims to identify the suitable subculturing time, culture media (protocols) and explants type suitable for somatic embryogenesis expression in five elite genotypes of Theobroma cacao L. Thus, petals and staminodes explants extracted from immature flower buds of genotypes (C1, C8, C9, C14, and C16) are first grown in different media (CIM and CDM) to assess the subculturing time. Then, successive transfers in EDM medium at every 4 weeks intervals until 24 weeks. To assess culture media preference, these genotypes were grown in four different protocols (I, II, III, and IV) for callus induction. They were then successively subcultured (four times at 4 weeks intervals) into EDM. About the subculture time, embryo expression is earlier in C1, C9, C14, and C16 genotypes (2nd and 4th weeks) than C8 genotype (12 and 16th weeks). However, C1, C14, and C16 (with staminodes explants) prefer protocol IV, while their petal explants prefer protocol II. Also, C9 staminodes explants prefer protocol III while the C9 and C8 petals explants prefer protocol I. This study has identified suitable explants and protocols for somatic embryo production in the tested genotypes. Moreover, this work has laid out a new strategy to overcome recalcitrance to the somatic embryogenesis of T. cacao genotypes.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"74 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139130312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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