Research on production and testing of antifungal activity Phytophthora spp. causing fruit rot and Colletotrichum spp. causing anthracnose of silver nanoparticles, chitosan and turmeric resin bioproducts

Abstract

In this paper, the research team made samples of bio-products (BN) of turmeric resin combined with silver nanoparticles and chitosan and investigated the factors affecting the production of BN preparations. Specifically, the results of research on the production of BN preparations including ingredients, additives, mixing ratios, and technological parameters have been calculated and tested to obtain nano-preparations with the highest durability and dispersion. The experimental results obtained the optimal set of parameters for production at a pilot scale of 5 litres/batch are the ratio of active ingredients/additives =3/1, the optimal temperature for the mixing process is 40-45oC, the stirring speed is 500 rpm, and the time is 60 minutes. From here, we have successfully fabricated 18 samples of BN1-BN18 preparations with colours ranging from light yellow to dark yellow. Antifungal activity was tested against Colletotrichum spp. causing anthracnose disease and fruit rot disease caused by Phytophthora spp., the results showed that BN1-BN18 products exhibited good antifungal activity, and antifungal activity increased gradually when the content of turmeric oil resin increased.