Fatty acid profiling of enterococcal isolates by Fames analysis with reference to antibiotic resistance from clinical samples collected in the Chandrapur region

Ashwini S. Muttawar, Vijay S. Wadhai
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Abstract

FAME Analysis is a simple and rapid technique based on Gas Chromatography analysis of bacterial cell membrane. Biochemical lipid profiling of bacterial cell membrane helps to identify bacterial isolates using MIDI Sherlock system. The main objective of present study is rapid and accurate identification of   multidrug resistant Enterococcal isolates and to study their clinical profile for complicated nosocomial infection from the clinical samples collected at tertiary care center in Chandrapur region. It is essential to identify the causative organism for proper diagnosis and treatment of diseases as enterococcus is fastly emerging pathogen responsible for life threating nosocomial infection and other health hazards.
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通过 Fames 分析法对钱德拉布尔地区采集的临床样本中分离出的肠球菌进行脂肪酸谱分析,并参考抗生素耐药性的情况
FAME 分析是一种基于细菌细胞膜气相色谱分析的简单而快速的技术。细菌细胞膜的生化脂质分析有助于使用 MIDI Sherlock 系统识别细菌分离物。本研究的主要目的是从 Chandrapur 地区三级医疗中心采集的临床样本中快速准确地鉴定耐多药肠道球菌分离株,并研究其在复杂的院内感染中的临床特征。由于肠球菌是一种快速出现的病原体,是威胁生命的院内感染和其他健康危害的罪魁祸首,因此鉴定致病菌对正确诊断和治疗疾病至关重要。
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