METABOLITES PROFILING AND IN VITRO ANTICANCER AND ANTIOXIDANT ACTIVITIES OF THREE DIFFERENT MARINE SPONGES METHANOLIC EXTRACTS

Abstract

In the current study, three different marine sponges methanolic extracts Clathria arbuscula ( SR) , Crella cyathophora (S), and Xenia macrspiculata (CS) were collected from the Red Sea in Hurghada and examined for their metabolite profiles, antioxidant, and anticancer properties. Flavonoid, total phenolic , (2,2-diphenyl-1-picrylhydrazyl )DPPH, and ( Ferric Reducing Antioxidant Power)FRAP tests for antioxidants were used to identify the different types of marine sponges methanolic extracts. The anticancer action of the cancer cells MDA-MB 231, HUH-7, and NB4-APL was dose-and extract-dependent. Total phenolic content (TPC) of Xenia macrspiculata methanolic extract has been found to be higher than that of Clathria arbuscula methanolic extract and Crella cyathophora methanolic extract . While the total flavonoid content (TFC) of Xenia macrspiculata methanolic extract has been shown to be higher than that of Clathria arbuscula methanolic extract and Crella cyathophora methanolic extract. Different levels of FRAP and DPPH activity have been found in all methanolic extracts of marine sponges. It has been worked the UPLC-TOF-MS analysis for the three different extracts. A total of 1