Synergistic anti-microbial effect of grilling and edible coating on spore forming B. cereus in beef fillets

Nahla A. Abo EL-Roos, Asmaa A. Hashhash, Mohebat A. Abd El-Aziz, El Sayed, Abd El
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Abstract

Q uality and safety of refrigerated beef fillet represented a concern for consumers. Moreover, synergistic effect between two or more of anti-microbial protocols to control bacteria in food especially heat resistant spore forming bacteria is a demand for food safety. So, aim of this study was to evaluate the synergistic application of grilling (heat treatment), and CMC coated samples supported by lactoferrin and propolis on survival of Bacillus cereus in beef fillets. Results revealed an effective synergistic antimicrobial effect of LF and PR incorporated into the CMC edible coating in a combination with grilling against experimentally inoculated B. cereus. So, grilling alone as antimicrobial can't eliminate B. cereus in beef fillets, while application of edible coating fortified with LF and PR enhanced the antimicrobial effect of grilling in a synergistic way to control B. cereus .
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烧烤和食用涂层对牛肉片中芽孢杆菌孢子形成的协同抗微生物作用
冷藏牛肉片的质量和安全是消费者关心的问题。此外,两种或多种抗微生物方案之间的协同效应可控制食品中的细菌,尤其是耐热孢子形成菌,这也是食品安全的要求。因此,本研究旨在评估烧烤(热处理)与乳铁蛋白和蜂胶支持的 CMC 涂层样品对牛肉片中蜡样芽孢杆菌存活的协同作用。结果表明,在 CMC 可食用涂层中加入乳铁蛋白和蜂胶,并与烧烤结合使用,对实验接种的蜡样芽孢杆菌具有有效的协同抗菌效果。因此,仅靠烧烤作为抗菌剂并不能消灭牛柳中的蜡样芽孢杆菌,而添加了 LF 和 PR 的可食用涂层则以协同增效的方式增强了烧烤的抗菌效果,从而控制了蜡样芽孢杆菌。
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